Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02405 and RR42_RS02410 are separated by 210 nucleotides RR42_RS02410 and RR42_RS02415 are separated by 105 nucleotides
RR42_RS02405: RR42_RS02405 - membrane protein, at 541,403 to 542,404
_RS02405
RR42_RS02410: RR42_RS02410 - sterol-binding protein, at 542,615 to 543,289
_RS02410
RR42_RS02415: RR42_RS02415 - ubiquinone biosynthesis protein UbiB, at 543,395 to 544,972
_RS02415
Position (kb) 542
543
544 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 541.633 kb on + strand, within RR42_RS02405 at 541.634 kb on - strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.079 kb on - strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.082 kb on - strand, within RR42_RS02405 at 542.082 kb on - strand, within RR42_RS02405 at 542.082 kb on - strand, within RR42_RS02405 at 542.085 kb on + strand, within RR42_RS02405 at 542.085 kb on + strand, within RR42_RS02405 at 542.086 kb on - strand, within RR42_RS02405 at 542.086 kb on - strand, within RR42_RS02405 at 542.432 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.451 kb on - strand at 542.451 kb on - strand at 542.451 kb on - strand at 542.522 kb on + strand at 542.522 kb on + strand at 542.526 kb on + strand at 542.582 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 541,633 + RR42_RS02405 0.23 -0.5 541,634 - RR42_RS02405 0.23 -2.0 542,078 + RR42_RS02405 0.67 -1.9 542,078 + RR42_RS02405 0.67 -1.0 542,078 + RR42_RS02405 0.67 -0.1 542,078 + RR42_RS02405 0.67 -1.0 542,079 - RR42_RS02405 0.67 +0.8 542,081 + RR42_RS02405 0.68 -2.6 542,081 + RR42_RS02405 0.68 -0.3 542,081 + RR42_RS02405 0.68 -0.7 542,081 + RR42_RS02405 0.68 -1.1 542,082 - RR42_RS02405 0.68 -1.6 542,082 - RR42_RS02405 0.68 -1.0 542,082 - RR42_RS02405 0.68 -0.2 542,085 + RR42_RS02405 0.68 -3.4 542,085 + RR42_RS02405 0.68 -2.3 542,086 - RR42_RS02405 0.68 -3.8 542,086 - RR42_RS02405 0.68 -1.5 542,432 + +1.0 542,450 + -0.1 542,450 + -0.4 542,450 + -1.4 542,450 + -0.6 542,450 + -0.5 542,450 + -0.2 542,450 + -0.2 542,450 + -0.3 542,450 + +0.8 542,450 + -0.6 542,450 + -0.1 542,451 - -1.0 542,451 - +0.5 542,451 - +0.4 542,522 + -0.2 542,522 + -1.8 542,526 + +0.3 542,582 + -0.4
Or see this region's nucleotide sequence