Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02375 and RR42_RS02380 are separated by 158 nucleotides RR42_RS02380 and RR42_RS02385 are separated by 70 nucleotides RR42_RS02385 and RR42_RS02390 are separated by 170 nucleotides
RR42_RS02375: RR42_RS02375 - membrane protein, at 532,816 to 533,394
_RS02375
RR42_RS02380: RR42_RS02380 - FAD-linked oxidase, at 533,553 to 537,527
_RS02380
RR42_RS02385: RR42_RS02385 - hydrolase, at 537,598 to 538,032
_RS02385
RR42_RS02390: RR42_RS02390 - ABC transporter ATP-binding protein, at 538,203 to 540,086
_RS02390
Position (kb) 533
534
535
536
537
538 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 532.554 kb on - strand at 532.626 kb on + strand at 532.627 kb on - strand at 532.627 kb on - strand at 532.627 kb on - strand at 532.731 kb on - strand at 532.731 kb on - strand at 532.813 kb on + strand at 532.813 kb on + strand at 532.813 kb on + strand at 532.814 kb on - strand at 532.815 kb on + strand at 532.815 kb on + strand at 532.815 kb on + strand at 532.819 kb on - strand at 532.946 kb on - strand, within RR42_RS02375 at 532.947 kb on + strand, within RR42_RS02375 at 532.948 kb on - strand, within RR42_RS02375 at 532.948 kb on - strand, within RR42_RS02375 at 532.948 kb on - strand, within RR42_RS02375 at 533.161 kb on + strand, within RR42_RS02375 at 533.162 kb on - strand, within RR42_RS02375 at 533.284 kb on + strand, within RR42_RS02375 at 533.284 kb on + strand, within RR42_RS02375 at 533.284 kb on + strand, within RR42_RS02375 at 533.285 kb on - strand, within RR42_RS02375 at 533.285 kb on - strand, within RR42_RS02375 at 533.285 kb on - strand, within RR42_RS02375 at 533.312 kb on - strand, within RR42_RS02375 at 533.312 kb on - strand, within RR42_RS02375 at 533.392 kb on + strand at 533.392 kb on + strand at 533.392 kb on + strand at 533.392 kb on + strand at 533.392 kb on + strand at 533.487 kb on - strand at 533.487 kb on - strand at 533.626 kb on - strand at 533.745 kb on + strand at 534.001 kb on - strand, within RR42_RS02380 at 534.120 kb on + strand, within RR42_RS02380 at 534.229 kb on - strand, within RR42_RS02380 at 535.258 kb on - strand, within RR42_RS02380 at 535.380 kb on - strand, within RR42_RS02380 at 536.026 kb on - strand, within RR42_RS02380 at 536.027 kb on + strand, within RR42_RS02380 at 536.176 kb on - strand, within RR42_RS02380 at 536.524 kb on + strand, within RR42_RS02380 at 536.740 kb on - strand, within RR42_RS02380 at 536.813 kb on - strand, within RR42_RS02380 at 536.813 kb on - strand, within RR42_RS02380 at 536.841 kb on + strand, within RR42_RS02380 at 536.950 kb on - strand, within RR42_RS02380 at 536.950 kb on - strand, within RR42_RS02380 at 536.992 kb on - strand, within RR42_RS02380 at 536.992 kb on - strand, within RR42_RS02380 at 537.061 kb on - strand, within RR42_RS02380 at 537.108 kb on + strand, within RR42_RS02380 at 537.109 kb on - strand, within RR42_RS02380 at 537.114 kb on + strand, within RR42_RS02380 at 537.114 kb on + strand, within RR42_RS02380 at 537.115 kb on - strand, within RR42_RS02380 at 537.115 kb on - strand, within RR42_RS02380 at 537.115 kb on - strand, within RR42_RS02380 at 537.115 kb on - strand, within RR42_RS02380 at 537.396 kb on + strand at 537.859 kb on - strand, within RR42_RS02385 at 537.882 kb on + strand, within RR42_RS02385 at 537.883 kb on - strand, within RR42_RS02385 at 538.130 kb on - strand at 538.130 kb on - strand at 538.311 kb on + strand at 538.311 kb on + strand at 538.312 kb on - strand at 538.386 kb on + strand at 538.387 kb on - strand at 538.387 kb on - strand at 538.388 kb on + strand at 538.388 kb on + strand at 538.389 kb on - strand at 538.389 kb on - strand at 538.389 kb on - strand at 538.426 kb on - strand, within RR42_RS02390 at 538.449 kb on + strand, within RR42_RS02390 at 538.449 kb on + strand, within RR42_RS02390 at 538.450 kb on - strand, within RR42_RS02390 at 538.450 kb on - strand, within RR42_RS02390 at 538.502 kb on + strand, within RR42_RS02390 at 538.503 kb on - strand, within RR42_RS02390 at 538.516 kb on + strand, within RR42_RS02390 at 538.517 kb on - strand, within RR42_RS02390 at 538.517 kb on - strand, within RR42_RS02390 at 538.517 kb on - strand, within RR42_RS02390 at 538.527 kb on + strand, within RR42_RS02390 at 538.527 kb on + strand, within RR42_RS02390
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 532,554 - -0.4 532,626 + -0.5 532,627 - +0.6 532,627 - -0.2 532,627 - +1.0 532,731 - +0.3 532,731 - -0.7 532,813 + -0.2 532,813 + -0.7 532,813 + +1.1 532,814 - -0.4 532,815 + -0.7 532,815 + -0.9 532,815 + -0.4 532,819 - -2.3 532,946 - RR42_RS02375 0.22 -0.8 532,947 + RR42_RS02375 0.23 +0.1 532,948 - RR42_RS02375 0.23 +0.6 532,948 - RR42_RS02375 0.23 -2.1 532,948 - RR42_RS02375 0.23 -2.1 533,161 + RR42_RS02375 0.60 -1.3 533,162 - RR42_RS02375 0.60 -0.6 533,284 + RR42_RS02375 0.81 -0.7 533,284 + RR42_RS02375 0.81 +0.2 533,284 + RR42_RS02375 0.81 -1.1 533,285 - RR42_RS02375 0.81 -0.8 533,285 - RR42_RS02375 0.81 -2.5 533,285 - RR42_RS02375 0.81 -0.5 533,312 - RR42_RS02375 0.86 -0.8 533,312 - RR42_RS02375 0.86 +0.0 533,392 + +0.2 533,392 + -0.2 533,392 + -2.3 533,392 + +1.2 533,392 + -0.5 533,487 - +0.2 533,487 - -0.2 533,626 - -0.6 533,745 + -2.1 534,001 - RR42_RS02380 0.11 -2.3 534,120 + RR42_RS02380 0.14 -0.6 534,229 - RR42_RS02380 0.17 -1.1 535,258 - RR42_RS02380 0.43 -1.3 535,380 - RR42_RS02380 0.46 -1.0 536,026 - RR42_RS02380 0.62 -0.2 536,027 + RR42_RS02380 0.62 -1.1 536,176 - RR42_RS02380 0.66 -2.2 536,524 + RR42_RS02380 0.75 -1.2 536,740 - RR42_RS02380 0.80 -1.2 536,813 - RR42_RS02380 0.82 +0.0 536,813 - RR42_RS02380 0.82 +0.2 536,841 + RR42_RS02380 0.83 -1.1 536,950 - RR42_RS02380 0.85 -1.3 536,950 - RR42_RS02380 0.85 +0.8 536,992 - RR42_RS02380 0.87 -0.6 536,992 - RR42_RS02380 0.87 -0.9 537,061 - RR42_RS02380 0.88 -0.8 537,108 + RR42_RS02380 0.89 -1.3 537,109 - RR42_RS02380 0.89 +0.2 537,114 + RR42_RS02380 0.90 +0.7 537,114 + RR42_RS02380 0.90 -1.6 537,115 - RR42_RS02380 0.90 +0.7 537,115 - RR42_RS02380 0.90 -0.8 537,115 - RR42_RS02380 0.90 -3.3 537,115 - RR42_RS02380 0.90 +0.5 537,396 + -1.6 537,859 - RR42_RS02385 0.60 +1.2 537,882 + RR42_RS02385 0.65 -0.5 537,883 - RR42_RS02385 0.66 -0.6 538,130 - -1.4 538,130 - -0.9 538,311 + -1.2 538,311 + +0.7 538,312 - +0.3 538,386 + +0.7 538,387 - +2.8 538,387 - -1.2 538,388 + -2.3 538,388 + +0.1 538,389 - +0.7 538,389 - -0.1 538,389 - -0.7 538,426 - RR42_RS02390 0.12 -0.2 538,449 + RR42_RS02390 0.13 +0.1 538,449 + RR42_RS02390 0.13 -1.1 538,450 - RR42_RS02390 0.13 -0.3 538,450 - RR42_RS02390 0.13 +0.7 538,502 + RR42_RS02390 0.16 -0.1 538,503 - RR42_RS02390 0.16 -0.2 538,516 + RR42_RS02390 0.17 -0.8 538,517 - RR42_RS02390 0.17 -0.6 538,517 - RR42_RS02390 0.17 +0.1 538,517 - RR42_RS02390 0.17 +0.2 538,527 + RR42_RS02390 0.17 +0.3 538,527 + RR42_RS02390 0.17 -0.4
Or see this region's nucleotide sequence