Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01680 and RR42_RS01685 are separated by 72 nucleotides RR42_RS01685 and RR42_RS01690 are separated by 97 nucleotides RR42_RS01690 and RR42_RS01695 are separated by 407 nucleotides
RR42_RS01680: RR42_RS01680 - cytochrome C550, at 382,374 to 383,582
_RS01680
RR42_RS01685: RR42_RS01685 - alpha/beta hydrolase, at 383,655 to 384,299
_RS01685
RR42_RS01690: RR42_RS01690 - ferredoxin, at 384,397 to 384,717
_RS01690
RR42_RS01695: RR42_RS01695 - C4-dicarboxylate transporter, at 385,125 to 386,426
_RS01695
Position (kb) 384
385 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 383.573 kb on + strand at 384.275 kb on + strand at 384.276 kb on - strand at 384.280 kb on - strand at 384.280 kb on - strand at 384.280 kb on - strand at 384.287 kb on + strand at 384.488 kb on + strand, within RR42_RS01690 at 384.489 kb on - strand, within RR42_RS01690 at 384.506 kb on + strand, within RR42_RS01690 at 384.618 kb on - strand, within RR42_RS01690 at 384.639 kb on + strand, within RR42_RS01690 at 384.704 kb on + strand at 384.705 kb on - strand at 384.705 kb on - strand at 384.789 kb on - strand at 384.789 kb on - strand at 384.806 kb on + strand at 384.806 kb on + strand at 384.807 kb on - strand at 384.807 kb on - strand at 384.807 kb on - strand at 384.808 kb on + strand at 384.809 kb on - strand at 385.046 kb on - strand at 385.143 kb on + strand at 385.144 kb on - strand at 385.155 kb on + strand at 385.156 kb on - strand at 385.156 kb on - strand at 385.223 kb on - strand at 385.307 kb on + strand, within RR42_RS01695 at 385.365 kb on + strand, within RR42_RS01695 at 385.485 kb on + strand, within RR42_RS01695 at 385.485 kb on + strand, within RR42_RS01695 at 385.485 kb on + strand, within RR42_RS01695 at 385.595 kb on - strand, within RR42_RS01695 at 385.703 kb on + strand, within RR42_RS01695 at 385.704 kb on - strand, within RR42_RS01695 at 385.704 kb on - strand, within RR42_RS01695 at 385.704 kb on - strand, within RR42_RS01695 at 385.704 kb on - strand, within RR42_RS01695
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 383,573 + +5.7 384,275 + +4.7 384,276 - +0.2 384,280 - +0.4 384,280 - -0.4 384,280 - -0.5 384,287 + +4.5 384,488 + RR42_RS01690 0.28 +5.2 384,489 - RR42_RS01690 0.29 -2.2 384,506 + RR42_RS01690 0.34 +4.8 384,618 - RR42_RS01690 0.69 +0.6 384,639 + RR42_RS01690 0.75 +1.6 384,704 + +2.3 384,705 - -0.6 384,705 - -0.1 384,789 - -0.2 384,789 - -0.3 384,806 + +0.9 384,806 + +1.8 384,807 - +0.3 384,807 - +0.2 384,807 - +0.2 384,808 + +0.3 384,809 - +0.1 385,046 - +0.1 385,143 + -0.1 385,144 - +0.8 385,155 + -0.1 385,156 - -1.2 385,156 - +0.9 385,223 - -0.1 385,307 + RR42_RS01695 0.14 +0.2 385,365 + RR42_RS01695 0.18 +0.3 385,485 + RR42_RS01695 0.28 -0.3 385,485 + RR42_RS01695 0.28 -0.7 385,485 + RR42_RS01695 0.28 +0.0 385,595 - RR42_RS01695 0.36 +0.5 385,703 + RR42_RS01695 0.44 +0.3 385,704 - RR42_RS01695 0.44 +0.9 385,704 - RR42_RS01695 0.44 +1.9 385,704 - RR42_RS01695 0.44 +1.3 385,704 - RR42_RS01695 0.44 +0.6
Or see this region's nucleotide sequence