Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01600 and RR42_RS01605 are separated by 111 nucleotides RR42_RS01605 and RR42_RS01610 are separated by 72 nucleotides
RR42_RS01600: RR42_RS01600 - methylmalonyl-CoA mutase, at 362,224 to 365,532
_RS01600
RR42_RS01605: RR42_RS01605 - hydrolase, at 365,644 to 366,078
_RS01605
RR42_RS01610: RR42_RS01610 - 3-hydroxybutyryl-CoA dehydrogenase, at 366,151 to 367,005
_RS01610
Position (kb) 365
366
367 Strain fitness (log2 ratio) -1
0
1
2
3 at 364.655 kb on - strand, within RR42_RS01600 at 364.667 kb on - strand, within RR42_RS01600 at 364.802 kb on - strand, within RR42_RS01600 at 364.834 kb on + strand, within RR42_RS01600 at 364.835 kb on - strand, within RR42_RS01600 at 365.209 kb on + strand at 365.213 kb on - strand at 365.213 kb on - strand at 365.259 kb on + strand at 365.259 kb on + strand at 365.260 kb on - strand at 365.505 kb on - strand at 365.526 kb on + strand at 365.527 kb on - strand at 365.572 kb on + strand at 365.653 kb on - strand at 365.674 kb on - strand at 365.674 kb on - strand at 365.717 kb on + strand, within RR42_RS01605 at 365.717 kb on + strand, within RR42_RS01605 at 365.718 kb on - strand, within RR42_RS01605 at 365.718 kb on - strand, within RR42_RS01605 at 365.720 kb on - strand, within RR42_RS01605 at 365.777 kb on + strand, within RR42_RS01605 at 365.778 kb on - strand, within RR42_RS01605 at 366.237 kb on - strand, within RR42_RS01610 at 366.237 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.380 kb on - strand, within RR42_RS01610 at 366.758 kb on + strand, within RR42_RS01610 at 366.893 kb on - strand, within RR42_RS01610 at 366.893 kb on - strand, within RR42_RS01610 at 366.989 kb on - strand at 366.989 kb on - strand at 366.994 kb on + strand at 366.994 kb on + strand at 366.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 364,655 - RR42_RS01600 0.73 +1.3 364,667 - RR42_RS01600 0.74 -0.3 364,802 - RR42_RS01600 0.78 -1.6 364,834 + RR42_RS01600 0.79 +0.1 364,835 - RR42_RS01600 0.79 -0.1 365,209 + -0.3 365,213 - +0.2 365,213 - +1.6 365,259 + +0.5 365,259 + +0.6 365,260 - -0.4 365,505 - +1.5 365,526 + -1.5 365,527 - +0.0 365,572 + -0.2 365,653 - -0.7 365,674 - +0.3 365,674 - +0.3 365,717 + RR42_RS01605 0.17 +0.5 365,717 + RR42_RS01605 0.17 +3.4 365,718 - RR42_RS01605 0.17 +0.1 365,718 - RR42_RS01605 0.17 +0.4 365,720 - RR42_RS01605 0.17 +1.4 365,777 + RR42_RS01605 0.31 +1.1 365,778 - RR42_RS01605 0.31 -0.7 366,237 - RR42_RS01610 0.10 +3.3 366,237 - RR42_RS01610 0.10 +0.3 366,380 - RR42_RS01610 0.27 -0.6 366,380 - RR42_RS01610 0.27 -1.5 366,380 - RR42_RS01610 0.27 +0.4 366,380 - RR42_RS01610 0.27 -0.0 366,758 + RR42_RS01610 0.71 -0.4 366,893 - RR42_RS01610 0.87 -1.5 366,893 - RR42_RS01610 0.87 +0.2 366,989 - -0.3 366,989 - -0.7 366,994 + -0.9 366,994 + +0.3 366,995 - +0.2
Or see this region's nucleotide sequence