Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01585 and RR42_RS01590 are separated by 12 nucleotides RR42_RS01590 and RR42_RS01595 are separated by 183 nucleotides RR42_RS01595 and RR42_RS01600 are separated by 367 nucleotides
RR42_RS01585: RR42_RS01585 - amino acid permease, at 358,521 to 359,915
_RS01585
RR42_RS01590: RR42_RS01590 - GntR family transcriptional regulator, at 359,928 to 361,334
_RS01590
RR42_RS01595: RR42_RS01595 - hypothetical protein, at 361,518 to 361,856
_RS01595
RR42_RS01600: RR42_RS01600 - methylmalonyl-CoA mutase, at 362,224 to 365,532
_RS01600
Position (kb) 359
360
361
362 Strain fitness (log2 ratio) -2
-1
0
1
2 at 359.216 kb on + strand, within RR42_RS01585 at 359.319 kb on + strand, within RR42_RS01585 at 359.319 kb on + strand, within RR42_RS01585 at 359.319 kb on + strand, within RR42_RS01585 at 359.320 kb on - strand, within RR42_RS01585 at 359.320 kb on - strand, within RR42_RS01585 at 359.320 kb on - strand, within RR42_RS01585 at 359.406 kb on + strand, within RR42_RS01585 at 359.868 kb on + strand at 359.868 kb on + strand at 359.869 kb on - strand at 359.869 kb on - strand at 360.037 kb on + strand at 360.113 kb on + strand, within RR42_RS01590 at 360.114 kb on - strand, within RR42_RS01590 at 360.151 kb on + strand, within RR42_RS01590 at 360.151 kb on + strand, within RR42_RS01590 at 360.232 kb on + strand, within RR42_RS01590 at 360.232 kb on + strand, within RR42_RS01590 at 360.232 kb on + strand, within RR42_RS01590 at 360.232 kb on + strand, within RR42_RS01590 at 360.233 kb on - strand, within RR42_RS01590 at 360.358 kb on + strand, within RR42_RS01590 at 360.407 kb on - strand, within RR42_RS01590 at 360.407 kb on - strand, within RR42_RS01590 at 360.452 kb on - strand, within RR42_RS01590 at 360.452 kb on - strand, within RR42_RS01590 at 360.452 kb on - strand, within RR42_RS01590 at 360.476 kb on + strand, within RR42_RS01590 at 360.477 kb on - strand, within RR42_RS01590 at 360.477 kb on - strand, within RR42_RS01590 at 360.710 kb on - strand, within RR42_RS01590 at 360.712 kb on + strand, within RR42_RS01590 at 360.737 kb on + strand, within RR42_RS01590 at 360.892 kb on + strand, within RR42_RS01590 at 361.031 kb on + strand, within RR42_RS01590 at 361.031 kb on + strand, within RR42_RS01590 at 361.032 kb on - strand, within RR42_RS01590 at 361.152 kb on + strand, within RR42_RS01590 at 361.165 kb on + strand, within RR42_RS01590 at 361.166 kb on - strand, within RR42_RS01590 at 361.414 kb on + strand at 361.415 kb on - strand at 361.430 kb on + strand at 361.430 kb on + strand at 361.431 kb on - strand at 361.431 kb on - strand at 361.431 kb on - strand at 361.528 kb on + strand at 361.529 kb on - strand at 361.529 kb on - strand at 361.912 kb on + strand at 361.912 kb on + strand at 361.918 kb on + strand at 361.918 kb on + strand at 361.919 kb on - strand at 361.919 kb on - strand at 361.919 kb on - strand at 362.022 kb on + strand at 362.023 kb on - strand at 362.023 kb on - strand at 362.039 kb on - strand at 362.039 kb on - strand at 362.039 kb on - strand at 362.095 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 359,216 + RR42_RS01585 0.50 +1.6 359,319 + RR42_RS01585 0.57 -0.2 359,319 + RR42_RS01585 0.57 +0.9 359,319 + RR42_RS01585 0.57 +0.1 359,320 - RR42_RS01585 0.57 -0.1 359,320 - RR42_RS01585 0.57 +0.4 359,320 - RR42_RS01585 0.57 -0.2 359,406 + RR42_RS01585 0.63 -1.5 359,868 + +0.3 359,868 + -0.9 359,869 - +0.3 359,869 - -1.9 360,037 + -1.0 360,113 + RR42_RS01590 0.13 +0.1 360,114 - RR42_RS01590 0.13 -0.2 360,151 + RR42_RS01590 0.16 -1.5 360,151 + RR42_RS01590 0.16 -0.7 360,232 + RR42_RS01590 0.22 +0.2 360,232 + RR42_RS01590 0.22 -1.5 360,232 + RR42_RS01590 0.22 -0.2 360,232 + RR42_RS01590 0.22 -0.4 360,233 - RR42_RS01590 0.22 -0.3 360,358 + RR42_RS01590 0.31 -0.1 360,407 - RR42_RS01590 0.34 -0.9 360,407 - RR42_RS01590 0.34 +1.5 360,452 - RR42_RS01590 0.37 +0.8 360,452 - RR42_RS01590 0.37 +0.8 360,452 - RR42_RS01590 0.37 +1.4 360,476 + RR42_RS01590 0.39 +2.1 360,477 - RR42_RS01590 0.39 +1.0 360,477 - RR42_RS01590 0.39 +0.2 360,710 - RR42_RS01590 0.56 -1.5 360,712 + RR42_RS01590 0.56 -1.0 360,737 + RR42_RS01590 0.57 +0.4 360,892 + RR42_RS01590 0.69 +1.1 361,031 + RR42_RS01590 0.78 -0.0 361,031 + RR42_RS01590 0.78 -0.4 361,032 - RR42_RS01590 0.78 +0.2 361,152 + RR42_RS01590 0.87 -0.2 361,165 + RR42_RS01590 0.88 -0.8 361,166 - RR42_RS01590 0.88 -0.7 361,414 + -0.2 361,415 - +0.8 361,430 + -0.5 361,430 + -1.5 361,431 - -0.0 361,431 - -0.7 361,431 - -1.3 361,528 + +0.5 361,529 - -0.5 361,529 - -0.2 361,912 + +0.2 361,912 + +0.0 361,918 + -0.4 361,918 + -0.9 361,919 - -1.4 361,919 - -0.8 361,919 - +0.3 362,022 + -0.6 362,023 - -0.1 362,023 - +1.4 362,039 - +0.1 362,039 - -0.5 362,039 - -1.0 362,095 + +0.4
Or see this region's nucleotide sequence