Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01040 and RR42_RS01045 are separated by 101 nucleotides RR42_RS01045 and RR42_RS01050 are separated by 147 nucleotides RR42_RS01050 and RR42_RS01055 are separated by 328 nucleotides
RR42_RS01040: RR42_RS01040 - cobalamin biosynthesis protein CobW, at 245,329 to 246,417
_RS01040
RR42_RS01045: RR42_RS01045 - Fur family transcriptional regulator, at 246,519 to 247,040
_RS01045
RR42_RS01050: RR42_RS01050 - alpha/beta hydrolase, at 247,188 to 248,087
_RS01050
RR42_RS01055: RR42_RS01055 - ABC transporter substrate-binding protein, at 248,416 to 249,402
_RS01055
Position (kb) 247
248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 246.310 kb on + strand at 246.311 kb on - strand at 246.345 kb on + strand at 246.346 kb on - strand at 246.346 kb on - strand at 246.464 kb on + strand at 246.520 kb on + strand at 246.521 kb on - strand at 246.811 kb on + strand, within RR42_RS01045 at 246.812 kb on - strand, within RR42_RS01045 at 247.050 kb on + strand at 247.050 kb on + strand at 247.051 kb on - strand at 247.051 kb on - strand at 247.056 kb on + strand at 247.057 kb on - strand at 247.147 kb on + strand at 247.147 kb on + strand at 247.147 kb on + strand at 247.147 kb on + strand at 247.148 kb on - strand at 247.148 kb on - strand at 247.188 kb on - strand at 247.188 kb on - strand at 247.244 kb on + strand at 247.245 kb on - strand at 247.440 kb on + strand, within RR42_RS01050 at 247.440 kb on + strand, within RR42_RS01050 at 247.440 kb on + strand, within RR42_RS01050 at 247.440 kb on + strand, within RR42_RS01050 at 247.440 kb on + strand, within RR42_RS01050 at 247.440 kb on + strand, within RR42_RS01050 at 247.440 kb on + strand, within RR42_RS01050 at 247.441 kb on - strand, within RR42_RS01050 at 247.441 kb on - strand, within RR42_RS01050 at 247.441 kb on - strand, within RR42_RS01050 at 247.511 kb on - strand, within RR42_RS01050 at 247.617 kb on + strand, within RR42_RS01050 at 247.617 kb on + strand, within RR42_RS01050 at 247.617 kb on + strand, within RR42_RS01050 at 247.617 kb on + strand, within RR42_RS01050 at 247.618 kb on - strand, within RR42_RS01050 at 247.618 kb on - strand, within RR42_RS01050 at 247.618 kb on - strand, within RR42_RS01050 at 247.618 kb on - strand, within RR42_RS01050 at 247.618 kb on - strand, within RR42_RS01050 at 247.618 kb on - strand, within RR42_RS01050 at 247.722 kb on + strand, within RR42_RS01050 at 247.722 kb on + strand, within RR42_RS01050 at 247.722 kb on + strand, within RR42_RS01050 at 247.722 kb on + strand, within RR42_RS01050 at 247.723 kb on - strand, within RR42_RS01050 at 247.723 kb on - strand, within RR42_RS01050 at 247.791 kb on + strand, within RR42_RS01050 at 247.792 kb on - strand, within RR42_RS01050 at 247.792 kb on - strand, within RR42_RS01050 at 247.842 kb on + strand, within RR42_RS01050 at 247.843 kb on - strand, within RR42_RS01050 at 248.028 kb on + strand at 248.028 kb on + strand at 248.028 kb on + strand at 248.028 kb on + strand at 248.029 kb on - strand at 248.029 kb on - strand at 248.029 kb on - strand at 248.029 kb on - strand at 248.165 kb on - strand at 248.214 kb on - strand at 248.236 kb on + strand at 248.237 kb on - strand at 248.237 kb on - strand at 248.237 kb on - strand at 248.237 kb on - strand at 248.237 kb on - strand at 248.237 kb on - strand at 248.278 kb on + strand at 248.278 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.393 kb on + strand at 248.394 kb on - strand at 248.394 kb on - strand at 248.394 kb on - strand at 248.412 kb on + strand at 248.683 kb on + strand, within RR42_RS01055 at 248.683 kb on + strand, within RR42_RS01055 at 248.683 kb on + strand, within RR42_RS01055 at 248.704 kb on - strand, within RR42_RS01055 at 248.704 kb on - strand, within RR42_RS01055 at 248.854 kb on + strand, within RR42_RS01055 at 248.854 kb on + strand, within RR42_RS01055 at 248.854 kb on + strand, within RR42_RS01055 at 248.854 kb on + strand, within RR42_RS01055 at 248.855 kb on - strand, within RR42_RS01055 at 248.855 kb on - strand, within RR42_RS01055 at 248.855 kb on - strand, within RR42_RS01055 at 248.856 kb on + strand, within RR42_RS01055 at 248.857 kb on - strand, within RR42_RS01055 at 248.857 kb on - strand, within RR42_RS01055 at 248.971 kb on + strand, within RR42_RS01055 at 248.972 kb on - strand, within RR42_RS01055 at 248.972 kb on - strand, within RR42_RS01055 at 249.082 kb on + strand, within RR42_RS01055 at 249.082 kb on + strand, within RR42_RS01055 at 249.082 kb on + strand, within RR42_RS01055 at 249.082 kb on + strand, within RR42_RS01055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 246,310 + -1.2 246,311 - +1.5 246,345 + +0.3 246,346 - +0.3 246,346 - -0.3 246,464 + -1.5 246,520 + +0.3 246,521 - +0.8 246,811 + RR42_RS01045 0.56 +0.8 246,812 - RR42_RS01045 0.56 +0.8 247,050 + -1.0 247,050 + +0.5 247,051 - -0.5 247,051 - -0.1 247,056 + -0.1 247,057 - -0.2 247,147 + -0.8 247,147 + +1.7 247,147 + -0.2 247,147 + +1.7 247,148 - -0.8 247,148 - +0.7 247,188 - -0.2 247,188 - +0.1 247,244 + -1.0 247,245 - -0.7 247,440 + RR42_RS01050 0.28 -0.7 247,440 + RR42_RS01050 0.28 -2.1 247,440 + RR42_RS01050 0.28 -0.6 247,440 + RR42_RS01050 0.28 -0.1 247,440 + RR42_RS01050 0.28 +0.8 247,440 + RR42_RS01050 0.28 +0.3 247,440 + RR42_RS01050 0.28 -0.5 247,441 - RR42_RS01050 0.28 +0.2 247,441 - RR42_RS01050 0.28 -0.4 247,441 - RR42_RS01050 0.28 +0.0 247,511 - RR42_RS01050 0.36 +0.0 247,617 + RR42_RS01050 0.48 +1.5 247,617 + RR42_RS01050 0.48 +0.3 247,617 + RR42_RS01050 0.48 -1.0 247,617 + RR42_RS01050 0.48 +0.8 247,618 - RR42_RS01050 0.48 +0.6 247,618 - RR42_RS01050 0.48 -0.2 247,618 - RR42_RS01050 0.48 -0.4 247,618 - RR42_RS01050 0.48 +0.1 247,618 - RR42_RS01050 0.48 -2.4 247,618 - RR42_RS01050 0.48 -0.4 247,722 + RR42_RS01050 0.59 -2.9 247,722 + RR42_RS01050 0.59 -0.8 247,722 + RR42_RS01050 0.59 +1.1 247,722 + RR42_RS01050 0.59 +1.1 247,723 - RR42_RS01050 0.59 +0.3 247,723 - RR42_RS01050 0.59 -0.2 247,791 + RR42_RS01050 0.67 +1.1 247,792 - RR42_RS01050 0.67 +1.2 247,792 - RR42_RS01050 0.67 +0.1 247,842 + RR42_RS01050 0.73 +0.0 247,843 - RR42_RS01050 0.73 +0.2 248,028 + +0.7 248,028 + +0.3 248,028 + -1.5 248,028 + +0.8 248,029 - -0.0 248,029 - +1.2 248,029 - -0.1 248,029 - -0.3 248,165 - -1.1 248,214 - +0.9 248,236 + +0.1 248,237 - +0.1 248,237 - +0.2 248,237 - +0.9 248,237 - +0.5 248,237 - -1.0 248,237 - -2.4 248,278 + -0.0 248,278 + -0.7 248,393 + +0.1 248,393 + -0.2 248,393 + +1.3 248,393 + +0.8 248,393 + -0.1 248,393 + -0.5 248,393 + +0.8 248,393 + -1.5 248,394 - +0.4 248,394 - -0.8 248,394 - -0.3 248,412 + -1.5 248,683 + RR42_RS01055 0.27 -0.1 248,683 + RR42_RS01055 0.27 -2.0 248,683 + RR42_RS01055 0.27 +0.5 248,704 - RR42_RS01055 0.29 -0.9 248,704 - RR42_RS01055 0.29 +0.8 248,854 + RR42_RS01055 0.44 -0.7 248,854 + RR42_RS01055 0.44 +0.8 248,854 + RR42_RS01055 0.44 +0.8 248,854 + RR42_RS01055 0.44 -0.3 248,855 - RR42_RS01055 0.44 +0.4 248,855 - RR42_RS01055 0.44 +0.5 248,855 - RR42_RS01055 0.44 +0.1 248,856 + RR42_RS01055 0.45 +0.7 248,857 - RR42_RS01055 0.45 -1.1 248,857 - RR42_RS01055 0.45 +0.6 248,971 + RR42_RS01055 0.56 +0.7 248,972 - RR42_RS01055 0.56 +0.2 248,972 - RR42_RS01055 0.56 +0.5 249,082 + RR42_RS01055 0.67 +0.4 249,082 + RR42_RS01055 0.67 +0.3 249,082 + RR42_RS01055 0.67 -0.4 249,082 + RR42_RS01055 0.67 +0.6
Or see this region's nucleotide sequence