Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00760 and RR42_RS00765 are separated by 149 nucleotides RR42_RS00765 and RR42_RS00770 are separated by 177 nucleotides RR42_RS00770 and RR42_RS00775 are separated by 35 nucleotides
RR42_RS00760: RR42_RS00760 - hypothetical protein, at 190,640 to 190,897
_RS00760
RR42_RS00765: RR42_RS00765 - sulfurtransferase, at 191,047 to 191,439
_RS00765
RR42_RS00770: RR42_RS00770 - 5-methyltetrahydrofolate--homocysteine methyltransferase, at 191,617 to 192,684
_RS00770
RR42_RS00775: RR42_RS00775 - methionine synthase, at 192,720 to 195,467
_RS00775
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 190.634 kb on + strand at 190.635 kb on - strand at 190.635 kb on - strand at 190.653 kb on - strand at 190.653 kb on - strand at 190.725 kb on - strand, within RR42_RS00760 at 190.864 kb on + strand, within RR42_RS00760 at 190.864 kb on + strand, within RR42_RS00760 at 190.865 kb on - strand, within RR42_RS00760 at 190.865 kb on - strand, within RR42_RS00760 at 190.895 kb on + strand at 190.896 kb on - strand at 190.896 kb on - strand at 190.964 kb on - strand at 190.998 kb on + strand at 190.998 kb on + strand at 191.051 kb on + strand at 191.051 kb on + strand at 191.052 kb on - strand at 191.052 kb on - strand at 191.052 kb on - strand at 191.094 kb on + strand, within RR42_RS00765 at 191.094 kb on + strand, within RR42_RS00765 at 191.094 kb on + strand, within RR42_RS00765 at 191.094 kb on + strand, within RR42_RS00765 at 191.095 kb on - strand, within RR42_RS00765 at 191.095 kb on - strand, within RR42_RS00765 at 191.147 kb on + strand, within RR42_RS00765 at 191.148 kb on - strand, within RR42_RS00765 at 191.351 kb on + strand, within RR42_RS00765 at 191.351 kb on + strand, within RR42_RS00765 at 191.352 kb on - strand, within RR42_RS00765 at 191.352 kb on - strand, within RR42_RS00765 at 191.352 kb on - strand, within RR42_RS00765 at 191.374 kb on + strand, within RR42_RS00765 at 191.487 kb on + strand at 191.573 kb on + strand at 191.573 kb on + strand at 191.573 kb on + strand at 191.574 kb on - strand at 191.574 kb on - strand at 191.662 kb on + strand at 191.663 kb on - strand at 191.746 kb on + strand, within RR42_RS00770 at 191.746 kb on + strand, within RR42_RS00770 at 191.747 kb on - strand, within RR42_RS00770 at 191.747 kb on - strand, within RR42_RS00770 at 191.747 kb on - strand, within RR42_RS00770 at 191.832 kb on - strand, within RR42_RS00770 at 191.869 kb on + strand, within RR42_RS00770 at 191.870 kb on - strand, within RR42_RS00770 at 191.904 kb on + strand, within RR42_RS00770 at 191.904 kb on + strand, within RR42_RS00770 at 191.904 kb on + strand, within RR42_RS00770 at 191.904 kb on + strand, within RR42_RS00770 at 191.905 kb on - strand, within RR42_RS00770 at 191.939 kb on - strand, within RR42_RS00770 at 191.939 kb on - strand, within RR42_RS00770 at 191.939 kb on - strand, within RR42_RS00770 at 192.011 kb on - strand, within RR42_RS00770 at 192.011 kb on - strand, within RR42_RS00770 at 192.011 kb on - strand, within RR42_RS00770 at 192.090 kb on - strand, within RR42_RS00770 at 192.090 kb on - strand, within RR42_RS00770 at 192.139 kb on + strand, within RR42_RS00770 at 192.140 kb on - strand, within RR42_RS00770 at 192.140 kb on - strand, within RR42_RS00770 at 192.142 kb on + strand, within RR42_RS00770 at 192.143 kb on - strand, within RR42_RS00770 at 192.143 kb on - strand, within RR42_RS00770 at 192.421 kb on + strand, within RR42_RS00770 at 192.421 kb on + strand, within RR42_RS00770 at 192.421 kb on + strand, within RR42_RS00770 at 192.609 kb on + strand at 193.246 kb on - strand, within RR42_RS00775 at 193.246 kb on - strand, within RR42_RS00775 at 193.246 kb on - strand, within RR42_RS00775 at 193.344 kb on + strand, within RR42_RS00775 at 193.396 kb on - strand, within RR42_RS00775 at 193.396 kb on - strand, within RR42_RS00775 at 193.427 kb on - strand, within RR42_RS00775 at 193.485 kb on + strand, within RR42_RS00775 at 193.546 kb on - strand, within RR42_RS00775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 190,634 + +0.9 190,635 - -0.3 190,635 - +1.2 190,653 - +0.7 190,653 - -0.9 190,725 - RR42_RS00760 0.33 -0.8 190,864 + RR42_RS00760 0.87 +0.7 190,864 + RR42_RS00760 0.87 -0.8 190,865 - RR42_RS00760 0.87 -1.3 190,865 - RR42_RS00760 0.87 +1.4 190,895 + +0.7 190,896 - -1.8 190,896 - -0.4 190,964 - -0.2 190,998 + +0.2 190,998 + -0.6 191,051 + -0.1 191,051 + -0.9 191,052 - -0.2 191,052 - +0.2 191,052 - +0.7 191,094 + RR42_RS00765 0.12 +0.1 191,094 + RR42_RS00765 0.12 -0.4 191,094 + RR42_RS00765 0.12 -1.0 191,094 + RR42_RS00765 0.12 -1.6 191,095 - RR42_RS00765 0.12 -0.1 191,095 - RR42_RS00765 0.12 +1.7 191,147 + RR42_RS00765 0.25 +1.4 191,148 - RR42_RS00765 0.26 -0.2 191,351 + RR42_RS00765 0.77 -1.0 191,351 + RR42_RS00765 0.77 -0.2 191,352 - RR42_RS00765 0.78 -0.3 191,352 - RR42_RS00765 0.78 -0.5 191,352 - RR42_RS00765 0.78 -0.7 191,374 + RR42_RS00765 0.83 +0.2 191,487 + -0.7 191,573 + -0.8 191,573 + -0.2 191,573 + -0.8 191,574 - +1.8 191,574 - +0.9 191,662 + -0.0 191,663 - +0.7 191,746 + RR42_RS00770 0.12 +0.1 191,746 + RR42_RS00770 0.12 +1.0 191,747 - RR42_RS00770 0.12 +0.4 191,747 - RR42_RS00770 0.12 +0.3 191,747 - RR42_RS00770 0.12 -0.6 191,832 - RR42_RS00770 0.20 +0.8 191,869 + RR42_RS00770 0.24 -1.1 191,870 - RR42_RS00770 0.24 +3.9 191,904 + RR42_RS00770 0.27 +0.0 191,904 + RR42_RS00770 0.27 -0.1 191,904 + RR42_RS00770 0.27 -0.5 191,904 + RR42_RS00770 0.27 +1.1 191,905 - RR42_RS00770 0.27 +0.8 191,939 - RR42_RS00770 0.30 -0.3 191,939 - RR42_RS00770 0.30 +0.8 191,939 - RR42_RS00770 0.30 +0.4 192,011 - RR42_RS00770 0.37 +0.1 192,011 - RR42_RS00770 0.37 -0.6 192,011 - RR42_RS00770 0.37 +0.5 192,090 - RR42_RS00770 0.44 +0.7 192,090 - RR42_RS00770 0.44 +0.7 192,139 + RR42_RS00770 0.49 +0.4 192,140 - RR42_RS00770 0.49 +1.5 192,140 - RR42_RS00770 0.49 +1.1 192,142 + RR42_RS00770 0.49 +0.3 192,143 - RR42_RS00770 0.49 +0.1 192,143 - RR42_RS00770 0.49 +1.4 192,421 + RR42_RS00770 0.75 +0.8 192,421 + RR42_RS00770 0.75 +0.4 192,421 + RR42_RS00770 0.75 -0.2 192,609 + +0.0 193,246 - RR42_RS00775 0.19 -0.6 193,246 - RR42_RS00775 0.19 -0.2 193,246 - RR42_RS00775 0.19 +0.5 193,344 + RR42_RS00775 0.23 +0.3 193,396 - RR42_RS00775 0.25 +1.1 193,396 - RR42_RS00775 0.25 -1.2 193,427 - RR42_RS00775 0.26 -0.8 193,485 + RR42_RS00775 0.28 -0.0 193,546 - RR42_RS00775 0.30 +0.8
Or see this region's nucleotide sequence