Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00520 and RR42_RS00525 are separated by 353 nucleotides RR42_RS00525 and RR42_RS00530 are separated by 146 nucleotides RR42_RS00530 and RR42_RS00535 are separated by 131 nucleotides
RR42_RS00520: RR42_RS00520 - ABC transporter substrate-binding protein, at 136,584 to 137,531
_RS00520
RR42_RS00525: RR42_RS00525 - MarR family transcriptional regulator, at 137,885 to 138,400
_RS00525
RR42_RS00530: RR42_RS00530 - hypothetical protein, at 138,547 to 139,239
_RS00530
RR42_RS00535: RR42_RS00535 - IMP dehydrogenase, at 139,371 to 142,913
_RS00535
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 137.076 kb on + strand, within RR42_RS00520 at 137.092 kb on + strand, within RR42_RS00520 at 137.134 kb on + strand, within RR42_RS00520 at 137.134 kb on + strand, within RR42_RS00520 at 137.135 kb on - strand, within RR42_RS00520 at 137.135 kb on - strand, within RR42_RS00520 at 137.135 kb on - strand, within RR42_RS00520 at 137.233 kb on + strand, within RR42_RS00520 at 137.233 kb on + strand, within RR42_RS00520 at 137.233 kb on + strand, within RR42_RS00520 at 137.248 kb on + strand, within RR42_RS00520 at 137.248 kb on + strand, within RR42_RS00520 at 137.248 kb on + strand, within RR42_RS00520 at 137.248 kb on + strand, within RR42_RS00520 at 137.327 kb on - strand, within RR42_RS00520 at 137.327 kb on - strand, within RR42_RS00520 at 137.327 kb on - strand at 137.550 kb on + strand at 137.623 kb on + strand at 137.623 kb on + strand at 137.624 kb on - strand at 137.781 kb on + strand at 137.782 kb on - strand at 137.922 kb on - strand at 137.930 kb on + strand at 137.931 kb on - strand at 137.931 kb on - strand at 138.200 kb on + strand, within RR42_RS00525 at 138.200 kb on + strand, within RR42_RS00525 at 138.398 kb on + strand at 138.446 kb on + strand at 138.447 kb on - strand at 138.447 kb on - strand at 138.454 kb on + strand at 138.454 kb on + strand at 138.454 kb on + strand at 138.455 kb on - strand at 138.743 kb on - strand, within RR42_RS00530 at 138.748 kb on + strand, within RR42_RS00530 at 138.748 kb on + strand, within RR42_RS00530 at 138.748 kb on + strand, within RR42_RS00530 at 138.749 kb on - strand, within RR42_RS00530 at 138.749 kb on - strand, within RR42_RS00530 at 138.749 kb on - strand, within RR42_RS00530 at 138.749 kb on - strand, within RR42_RS00530 at 138.749 kb on - strand, within RR42_RS00530 at 138.835 kb on - strand, within RR42_RS00530 at 138.835 kb on - strand, within RR42_RS00530 at 138.835 kb on - strand, within RR42_RS00530 at 138.868 kb on + strand, within RR42_RS00530 at 138.868 kb on + strand, within RR42_RS00530 at 138.868 kb on + strand, within RR42_RS00530 at 138.868 kb on + strand, within RR42_RS00530 at 138.868 kb on + strand, within RR42_RS00530 at 138.868 kb on + strand, within RR42_RS00530 at 138.976 kb on + strand, within RR42_RS00530 at 138.976 kb on + strand, within RR42_RS00530 at 138.977 kb on - strand, within RR42_RS00530 at 138.977 kb on - strand, within RR42_RS00530 at 139.007 kb on - strand, within RR42_RS00530 at 139.028 kb on - strand, within RR42_RS00530 at 139.030 kb on - strand, within RR42_RS00530 at 139.030 kb on - strand, within RR42_RS00530 at 139.066 kb on + strand, within RR42_RS00530 at 139.066 kb on + strand, within RR42_RS00530 at 139.066 kb on + strand, within RR42_RS00530 at 139.066 kb on + strand, within RR42_RS00530 at 139.067 kb on - strand, within RR42_RS00530 at 139.067 kb on - strand, within RR42_RS00530 at 139.067 kb on - strand, within RR42_RS00530 at 139.067 kb on - strand, within RR42_RS00530 at 139.081 kb on + strand, within RR42_RS00530 at 139.082 kb on - strand, within RR42_RS00530 at 139.084 kb on + strand, within RR42_RS00530 at 139.084 kb on + strand, within RR42_RS00530 at 139.085 kb on - strand, within RR42_RS00530 at 139.085 kb on - strand, within RR42_RS00530 at 139.085 kb on - strand, within RR42_RS00530 at 139.141 kb on + strand, within RR42_RS00530 at 139.237 kb on + strand at 139.237 kb on + strand at 139.237 kb on + strand at 139.237 kb on + strand at 139.237 kb on + strand at 139.237 kb on + strand at 139.237 kb on + strand at 139.238 kb on - strand at 139.238 kb on - strand at 139.238 kb on - strand at 139.238 kb on - strand at 139.238 kb on - strand at 139.249 kb on - strand at 139.318 kb on + strand at 139.318 kb on + strand at 139.318 kb on + strand at 139.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 137,076 + RR42_RS00520 0.52 -1.4 137,092 + RR42_RS00520 0.54 -1.0 137,134 + RR42_RS00520 0.58 -0.2 137,134 + RR42_RS00520 0.58 -1.3 137,135 - RR42_RS00520 0.58 -0.4 137,135 - RR42_RS00520 0.58 +1.1 137,135 - RR42_RS00520 0.58 +0.1 137,233 + RR42_RS00520 0.68 +1.2 137,233 + RR42_RS00520 0.68 +1.1 137,233 + RR42_RS00520 0.68 -0.9 137,248 + RR42_RS00520 0.70 +0.4 137,248 + RR42_RS00520 0.70 -0.6 137,248 + RR42_RS00520 0.70 -0.2 137,248 + RR42_RS00520 0.70 -0.2 137,327 - RR42_RS00520 0.78 -0.3 137,327 - RR42_RS00520 0.78 +0.1 137,327 - -0.6 137,550 + +0.5 137,623 + +0.0 137,623 + +0.5 137,624 - -0.3 137,781 + -0.8 137,782 - +0.3 137,922 - +0.0 137,930 + -0.6 137,931 - +0.8 137,931 - +0.7 138,200 + RR42_RS00525 0.61 -1.3 138,200 + RR42_RS00525 0.61 -0.8 138,398 + -0.5 138,446 + +0.3 138,447 - +0.2 138,447 - -2.5 138,454 + +0.2 138,454 + -1.0 138,454 + +0.0 138,455 - +0.5 138,743 - RR42_RS00530 0.28 -0.3 138,748 + RR42_RS00530 0.29 +0.8 138,748 + RR42_RS00530 0.29 -0.4 138,748 + RR42_RS00530 0.29 -0.9 138,749 - RR42_RS00530 0.29 -0.2 138,749 - RR42_RS00530 0.29 +0.9 138,749 - RR42_RS00530 0.29 -0.6 138,749 - RR42_RS00530 0.29 +0.9 138,749 - RR42_RS00530 0.29 -0.7 138,835 - RR42_RS00530 0.42 -0.8 138,835 - RR42_RS00530 0.42 -1.4 138,835 - RR42_RS00530 0.42 +1.8 138,868 + RR42_RS00530 0.46 +0.1 138,868 + RR42_RS00530 0.46 +0.1 138,868 + RR42_RS00530 0.46 +0.2 138,868 + RR42_RS00530 0.46 -0.6 138,868 + RR42_RS00530 0.46 -0.8 138,868 + RR42_RS00530 0.46 -0.5 138,976 + RR42_RS00530 0.62 +0.7 138,976 + RR42_RS00530 0.62 -0.1 138,977 - RR42_RS00530 0.62 -1.4 138,977 - RR42_RS00530 0.62 +0.7 139,007 - RR42_RS00530 0.66 +0.2 139,028 - RR42_RS00530 0.69 +0.7 139,030 - RR42_RS00530 0.70 -1.0 139,030 - RR42_RS00530 0.70 +0.7 139,066 + RR42_RS00530 0.75 +2.4 139,066 + RR42_RS00530 0.75 -0.8 139,066 + RR42_RS00530 0.75 +0.4 139,066 + RR42_RS00530 0.75 +0.5 139,067 - RR42_RS00530 0.75 +0.2 139,067 - RR42_RS00530 0.75 -0.0 139,067 - RR42_RS00530 0.75 +1.2 139,067 - RR42_RS00530 0.75 -0.9 139,081 + RR42_RS00530 0.77 +0.2 139,082 - RR42_RS00530 0.77 +0.1 139,084 + RR42_RS00530 0.77 -2.6 139,084 + RR42_RS00530 0.77 -0.1 139,085 - RR42_RS00530 0.78 -0.3 139,085 - RR42_RS00530 0.78 +0.7 139,085 - RR42_RS00530 0.78 -1.8 139,141 + RR42_RS00530 0.86 -1.9 139,237 + -0.4 139,237 + +0.7 139,237 + -0.1 139,237 + +0.5 139,237 + -0.5 139,237 + -0.5 139,237 + -0.3 139,238 - +0.3 139,238 - +0.5 139,238 - -0.5 139,238 - +0.0 139,238 - -0.1 139,249 - +1.0 139,318 + +0.3 139,318 + -0.7 139,318 + -0.9 139,370 - -0.3
Or see this region's nucleotide sequence