Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00450 and RR42_RS00455 are separated by 15 nucleotides RR42_RS00455 and RR42_RS00460 are separated by 62 nucleotides RR42_RS00460 and RR42_RS00465 are separated by 144 nucleotides
RR42_RS00450: RR42_RS00450 - cell division topological specificity factor MinE, at 117,050 to 117,304
_RS00450
RR42_RS00455: RR42_RS00455 - cell division inhibitor MinD, at 117,320 to 118,135
_RS00455
RR42_RS00460: RR42_RS00460 - septum formation inhibitor, at 118,198 to 119,013
_RS00460
RR42_RS00465: RR42_RS00465 - magnesium transporter, at 119,158 to 120,129
_RS00465
Position (kb) 118
119
120 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 117.322 kb on - strand at 117.322 kb on - strand at 117.363 kb on + strand at 117.420 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.589 kb on - strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.789 kb on + strand, within RR42_RS00455 at 117.789 kb on + strand, within RR42_RS00455 at 117.790 kb on - strand, within RR42_RS00455 at 117.945 kb on - strand, within RR42_RS00455 at 117.945 kb on - strand, within RR42_RS00455 at 117.963 kb on + strand, within RR42_RS00455 at 118.094 kb on + strand at 118.199 kb on + strand at 118.200 kb on - strand at 118.200 kb on - strand at 118.200 kb on - strand at 118.301 kb on + strand, within RR42_RS00460 at 118.406 kb on + strand, within RR42_RS00460 at 118.407 kb on - strand, within RR42_RS00460 at 118.407 kb on - strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.416 kb on + strand, within RR42_RS00460 at 118.445 kb on + strand, within RR42_RS00460 at 118.484 kb on + strand, within RR42_RS00460 at 118.485 kb on - strand, within RR42_RS00460 at 119.013 kb on + strand at 119.013 kb on + strand at 119.013 kb on + strand at 119.013 kb on + strand at 119.014 kb on - strand at 119.042 kb on + strand at 119.043 kb on - strand at 119.074 kb on - strand at 119.126 kb on + strand at 119.126 kb on + strand at 119.127 kb on - strand at 119.186 kb on - strand at 119.383 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.512 kb on + strand, within RR42_RS00465 at 119.512 kb on + strand, within RR42_RS00465 at 119.572 kb on + strand, within RR42_RS00465 at 119.572 kb on + strand, within RR42_RS00465 at 119.573 kb on - strand, within RR42_RS00465 at 119.581 kb on + strand, within RR42_RS00465 at 119.582 kb on - strand, within RR42_RS00465 at 119.611 kb on + strand, within RR42_RS00465 at 119.612 kb on - strand, within RR42_RS00465 at 119.612 kb on - strand, within RR42_RS00465 at 119.821 kb on + strand, within RR42_RS00465 at 120.004 kb on + strand, within RR42_RS00465 at 120.004 kb on + strand, within RR42_RS00465 at 120.004 kb on + strand, within RR42_RS00465
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 117,322 - +0.3 117,322 - -1.1 117,363 + -0.8 117,420 + RR42_RS00455 0.12 -1.6 117,588 + RR42_RS00455 0.33 +0.9 117,588 + RR42_RS00455 0.33 +2.4 117,588 + RR42_RS00455 0.33 -0.1 117,588 + RR42_RS00455 0.33 +0.1 117,589 - RR42_RS00455 0.33 +0.3 117,735 + RR42_RS00455 0.51 +0.1 117,735 + RR42_RS00455 0.51 +0.1 117,735 + RR42_RS00455 0.51 -2.8 117,735 + RR42_RS00455 0.51 +0.3 117,736 - RR42_RS00455 0.51 +0.3 117,736 - RR42_RS00455 0.51 -1.2 117,736 - RR42_RS00455 0.51 +0.3 117,789 + RR42_RS00455 0.57 -0.4 117,789 + RR42_RS00455 0.57 -0.2 117,790 - RR42_RS00455 0.58 -0.0 117,945 - RR42_RS00455 0.77 -0.4 117,945 - RR42_RS00455 0.77 +0.2 117,963 + RR42_RS00455 0.79 +0.5 118,094 + -1.9 118,199 + +0.0 118,200 - +0.4 118,200 - -0.8 118,200 - +1.0 118,301 + RR42_RS00460 0.13 -0.2 118,406 + RR42_RS00460 0.25 -0.5 118,407 - RR42_RS00460 0.26 -0.6 118,407 - RR42_RS00460 0.26 -0.1 118,410 + RR42_RS00460 0.26 -1.3 118,410 + RR42_RS00460 0.26 +0.6 118,410 + RR42_RS00460 0.26 -0.9 118,411 - RR42_RS00460 0.26 -0.6 118,411 - RR42_RS00460 0.26 -0.9 118,411 - RR42_RS00460 0.26 +0.4 118,411 - RR42_RS00460 0.26 +1.1 118,411 - RR42_RS00460 0.26 -1.1 118,416 + RR42_RS00460 0.27 -0.2 118,445 + RR42_RS00460 0.30 -0.0 118,484 + RR42_RS00460 0.35 -0.8 118,485 - RR42_RS00460 0.35 -1.5 119,013 + -0.6 119,013 + +0.0 119,013 + +2.4 119,013 + -0.2 119,014 - -0.3 119,042 + +1.5 119,043 - -2.2 119,074 - +0.2 119,126 + +0.5 119,126 + +0.8 119,127 - -0.4 119,186 - +0.7 119,383 + RR42_RS00465 0.23 +1.3 119,476 + RR42_RS00465 0.33 -0.9 119,476 + RR42_RS00465 0.33 -0.6 119,476 + RR42_RS00465 0.33 +0.4 119,476 + RR42_RS00465 0.33 +0.7 119,477 - RR42_RS00465 0.33 -1.8 119,477 - RR42_RS00465 0.33 -0.1 119,477 - RR42_RS00465 0.33 +0.3 119,477 - RR42_RS00465 0.33 +0.6 119,477 - RR42_RS00465 0.33 +0.0 119,477 - RR42_RS00465 0.33 -1.6 119,477 - RR42_RS00465 0.33 -1.6 119,477 - RR42_RS00465 0.33 +0.2 119,512 + RR42_RS00465 0.36 +2.5 119,512 + RR42_RS00465 0.36 -0.8 119,572 + RR42_RS00465 0.43 +0.4 119,572 + RR42_RS00465 0.43 +0.0 119,573 - RR42_RS00465 0.43 -0.2 119,581 + RR42_RS00465 0.44 -0.0 119,582 - RR42_RS00465 0.44 +0.3 119,611 + RR42_RS00465 0.47 -0.1 119,612 - RR42_RS00465 0.47 -1.4 119,612 - RR42_RS00465 0.47 -0.4 119,821 + RR42_RS00465 0.68 +0.8 120,004 + RR42_RS00465 0.87 +0.4 120,004 + RR42_RS00465 0.87 -2.8 120,004 + RR42_RS00465 0.87 +0.7
Or see this region's nucleotide sequence