Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00015 and RR42_RS00020 are separated by 364 nucleotides RR42_RS00020 and RR42_RS00025 are separated by 225 nucleotides
RR42_RS00015: RR42_RS00015 - DNA gyrase subunit B, at 4,224 to 6,761
_RS00015
RR42_RS00020: RR42_RS00020 - hypothetical protein, at 7,126 to 7,770
_RS00020
RR42_RS00025: RR42_RS00025 - GTPase, at 7,996 to 10,740
_RS00025
Position (kb) 7
8 Strain fitness (log2 ratio) -2
-1
0
1
2 at 6.838 kb on + strand at 6.863 kb on + strand at 6.931 kb on - strand at 6.952 kb on - strand at 7.032 kb on + strand at 7.033 kb on - strand at 7.037 kb on + strand at 7.037 kb on + strand at 7.093 kb on - strand at 7.098 kb on + strand at 7.099 kb on - strand at 7.099 kb on - strand at 7.151 kb on + strand at 7.304 kb on + strand, within RR42_RS00020 at 7.305 kb on - strand, within RR42_RS00020 at 7.326 kb on + strand, within RR42_RS00020 at 7.329 kb on + strand, within RR42_RS00020 at 7.352 kb on + strand, within RR42_RS00020 at 7.402 kb on - strand, within RR42_RS00020 at 7.607 kb on + strand, within RR42_RS00020 at 7.608 kb on - strand, within RR42_RS00020 at 7.608 kb on - strand, within RR42_RS00020 at 7.642 kb on - strand, within RR42_RS00020 at 7.663 kb on + strand, within RR42_RS00020 at 7.768 kb on + strand at 7.784 kb on + strand at 7.785 kb on - strand at 7.853 kb on - strand at 7.879 kb on - strand at 7.939 kb on + strand at 7.956 kb on + strand at 7.971 kb on + strand at 7.972 kb on - strand at 7.972 kb on - strand at 7.973 kb on + strand at 7.975 kb on + strand at 7.976 kb on - strand at 7.976 kb on - strand at 8.140 kb on + strand at 8.189 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.253 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.257 kb on + strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.258 kb on - strand at 8.312 kb on + strand, within RR42_RS00025 at 8.465 kb on + strand, within RR42_RS00025 at 8.465 kb on + strand, within RR42_RS00025 at 8.466 kb on - strand, within RR42_RS00025 at 8.466 kb on - strand, within RR42_RS00025 at 8.755 kb on + strand, within RR42_RS00025 at 8.755 kb on + strand, within RR42_RS00025 at 8.758 kb on + strand, within RR42_RS00025 at 8.758 kb on + strand, within RR42_RS00025 at 8.758 kb on + strand, within RR42_RS00025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 6,838 + -0.2 6,863 + -1.2 6,931 - +0.4 6,952 - -0.6 7,032 + -1.6 7,033 - -0.9 7,037 + -0.7 7,037 + +0.2 7,093 - +0.7 7,098 + +0.2 7,099 - +1.0 7,099 - -0.5 7,151 + +0.5 7,304 + RR42_RS00020 0.28 +0.3 7,305 - RR42_RS00020 0.28 +0.4 7,326 + RR42_RS00020 0.31 -0.1 7,329 + RR42_RS00020 0.31 -1.1 7,352 + RR42_RS00020 0.35 +0.8 7,402 - RR42_RS00020 0.43 -0.1 7,607 + RR42_RS00020 0.75 -1.4 7,608 - RR42_RS00020 0.75 +1.0 7,608 - RR42_RS00020 0.75 +0.1 7,642 - RR42_RS00020 0.80 +0.8 7,663 + RR42_RS00020 0.83 +2.4 7,768 + +0.5 7,784 + +0.3 7,785 - +1.0 7,853 - -0.6 7,879 - +0.2 7,939 + -0.4 7,956 + -1.8 7,971 + +0.9 7,972 - -0.6 7,972 - -1.8 7,973 + -0.3 7,975 + +1.4 7,976 - -1.9 7,976 - -0.1 8,140 + -0.4 8,189 + -0.2 8,253 + +0.3 8,253 + -0.8 8,253 + -0.4 8,253 + -0.5 8,253 + -1.4 8,253 + -0.8 8,257 + +1.0 8,257 + -0.1 8,257 + -1.8 8,257 + -0.0 8,257 + -0.0 8,257 + +0.3 8,258 - -0.2 8,258 - +1.4 8,258 - +1.4 8,258 - +0.2 8,258 - -2.0 8,312 + RR42_RS00025 0.12 -0.6 8,465 + RR42_RS00025 0.17 +0.1 8,465 + RR42_RS00025 0.17 -0.0 8,466 - RR42_RS00025 0.17 +1.8 8,466 - RR42_RS00025 0.17 -0.5 8,755 + RR42_RS00025 0.28 -0.6 8,755 + RR42_RS00025 0.28 +0.2 8,758 + RR42_RS00025 0.28 +0.6 8,758 + RR42_RS00025 0.28 -0.8 8,758 + RR42_RS00025 0.28 +0.5
Or see this region's nucleotide sequence