Experiment: KB with Ampicillin 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2077 and Psyr_2078 are separated by 125 nucleotides Psyr_2078 and Psyr_2079 are separated by 139 nucleotides Psyr_2079 and Psyr_2080 are separated by 134 nucleotides
Psyr_2077: Psyr_2077 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,422,181 to 2,423,155
_2077
Psyr_2078: Psyr_2078 - phosphoadenylylsulfate reductase (thioredoxin), at 2,423,281 to 2,424,015
_2078
Psyr_2079: Psyr_2079 - phosphoserine phosphatase / phosphoserine:homoserine phosphotransferase, at 2,424,155 to 2,424,772
_2079
Psyr_2080: Psyr_2080 - aminodeoxychorismate synthase, subunit I, at 2,424,907 to 2,426,250
_2080
Position (kb)
2424
2425 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2423.170 kb on + strand at 2423.170 kb on + strand at 2423.171 kb on - strand at 2423.171 kb on - strand at 2423.182 kb on + strand at 2423.182 kb on + strand at 2423.182 kb on + strand at 2423.183 kb on - strand at 2423.183 kb on - strand at 2423.183 kb on - strand at 2423.189 kb on + strand at 2423.189 kb on + strand at 2423.189 kb on + strand at 2423.190 kb on - strand at 2423.190 kb on - strand at 2423.190 kb on - strand at 2423.196 kb on + strand at 2423.196 kb on + strand at 2423.196 kb on + strand at 2423.196 kb on + strand at 2423.196 kb on + strand at 2423.197 kb on - strand at 2423.197 kb on - strand at 2423.197 kb on - strand at 2423.203 kb on + strand at 2423.203 kb on + strand at 2423.210 kb on + strand at 2423.210 kb on + strand at 2423.211 kb on - strand at 2423.211 kb on - strand at 2423.211 kb on - strand at 2423.211 kb on - strand at 2423.282 kb on + strand at 2424.029 kb on - strand at 2424.189 kb on + strand at 2424.189 kb on + strand at 2424.269 kb on + strand, within Psyr_2079 at 2424.269 kb on + strand, within Psyr_2079 at 2424.434 kb on + strand, within Psyr_2079 at 2424.435 kb on - strand, within Psyr_2079 at 2424.584 kb on + strand, within Psyr_2079 at 2424.584 kb on + strand, within Psyr_2079 at 2424.585 kb on - strand, within Psyr_2079 at 2424.588 kb on - strand, within Psyr_2079 at 2424.614 kb on + strand, within Psyr_2079 at 2424.712 kb on + strand at 2424.712 kb on + strand at 2424.712 kb on + strand at 2424.712 kb on + strand at 2425.085 kb on + strand, within Psyr_2080 at 2425.085 kb on + strand, within Psyr_2080 at 2425.085 kb on + strand, within Psyr_2080 at 2425.085 kb on + strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.086 kb on - strand, within Psyr_2080 at 2425.104 kb on - strand, within Psyr_2080 at 2425.668 kb on - strand, within Psyr_2080 at 2425.668 kb on - strand, within Psyr_2080 at 2425.668 kb on - strand, within Psyr_2080 at 2425.697 kb on + strand, within Psyr_2080 at 2425.698 kb on - strand, within Psyr_2080
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ampicillin 15.6 ug/ml remove 2,423,170 + -1.7 2,423,170 + -0.1 2,423,171 - -2.4 2,423,171 - -1.7 2,423,182 + +1.4 2,423,182 + +1.3 2,423,182 + -0.5 2,423,183 - -0.8 2,423,183 - -2.2 2,423,183 - +1.6 2,423,189 + +0.6 2,423,189 + -2.9 2,423,189 + +2.2 2,423,190 - -1.2 2,423,190 - +0.2 2,423,190 - -0.1 2,423,196 + -0.8 2,423,196 + -0.6 2,423,196 + +0.0 2,423,196 + +0.5 2,423,196 + -0.8 2,423,197 - +1.6 2,423,197 - +1.2 2,423,197 - -1.3 2,423,203 + +0.6 2,423,203 + -1.0 2,423,210 + +1.0 2,423,210 + -1.7 2,423,211 - -3.0 2,423,211 - -1.1 2,423,211 - +0.0 2,423,211 - -1.2 2,423,282 + +0.1 2,424,029 - -0.8 2,424,189 + +0.5 2,424,189 + -0.3 2,424,269 + Psyr_2079 0.18 +1.6 2,424,269 + Psyr_2079 0.18 -0.8 2,424,434 + Psyr_2079 0.45 -0.1 2,424,435 - Psyr_2079 0.45 -0.1 2,424,584 + Psyr_2079 0.69 -0.5 2,424,584 + Psyr_2079 0.69 +0.6 2,424,585 - Psyr_2079 0.70 +0.1 2,424,588 - Psyr_2079 0.70 -1.0 2,424,614 + Psyr_2079 0.74 -0.7 2,424,712 + -0.1 2,424,712 + +0.9 2,424,712 + +0.8 2,424,712 + +1.6 2,425,085 + Psyr_2080 0.13 -1.6 2,425,085 + Psyr_2080 0.13 +0.4 2,425,085 + Psyr_2080 0.13 -2.3 2,425,085 + Psyr_2080 0.13 -1.7 2,425,086 - Psyr_2080 0.13 -1.0 2,425,086 - Psyr_2080 0.13 -2.2 2,425,086 - Psyr_2080 0.13 +0.6 2,425,086 - Psyr_2080 0.13 -2.2 2,425,104 - Psyr_2080 0.15 -1.2 2,425,668 - Psyr_2080 0.57 -1.1 2,425,668 - Psyr_2080 0.57 -1.7 2,425,668 - Psyr_2080 0.57 -0.4 2,425,697 + Psyr_2080 0.59 -0.9 2,425,698 - Psyr_2080 0.59 +0.6
Or see this region's nucleotide sequence