Experiment: KB with Ampicillin 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0089 and Psyr_0090 are separated by 156 nucleotides Psyr_0090 and Psyr_0091 are separated by 179 nucleotides
Psyr_0089: Psyr_0089 - Response regulator receiver, at 96,943 to 98,331
_0089
Psyr_0090: Psyr_0090 - 4-aminobutyrate aminotransferase apoenzyme, at 98,488 to 99,768
_0090
Psyr_0091: Psyr_0091 - succinate semialdehyde dehydrogenase, at 99,948 to 101,390
_0091
Position (kb) 98
99
100 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 97.729 kb on + strand, within Psyr_0089 at 97.997 kb on + strand, within Psyr_0089 at 97.998 kb on - strand, within Psyr_0089 at 97.998 kb on - strand, within Psyr_0089 at 98.294 kb on - strand at 98.294 kb on - strand at 98.317 kb on + strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.382 kb on + strand at 98.399 kb on + strand at 98.399 kb on + strand at 98.400 kb on - strand at 98.400 kb on - strand at 98.400 kb on - strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.742 kb on - strand, within Psyr_0090 at 98.742 kb on - strand, within Psyr_0090 at 98.742 kb on - strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.973 kb on + strand, within Psyr_0090 at 98.974 kb on - strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.271 kb on - strand, within Psyr_0090 at 99.354 kb on - strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.967 kb on + strand at 99.967 kb on + strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.983 kb on - strand at 99.997 kb on + strand at 99.997 kb on + strand at 99.997 kb on + strand at 100.094 kb on - strand, within Psyr_0091 at 100.141 kb on + strand, within Psyr_0091 at 100.147 kb on + strand, within Psyr_0091 at 100.147 kb on + strand, within Psyr_0091 at 100.148 kb on - strand, within Psyr_0091 at 100.266 kb on - strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.547 kb on - strand, within Psyr_0091
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Ampicillin 15.6 ug/ml remove 97,729 + Psyr_0089 0.57 -0.3 97,997 + Psyr_0089 0.76 +0.0 97,998 - Psyr_0089 0.76 -0.4 97,998 - Psyr_0089 0.76 +0.5 98,294 - -1.5 98,294 - -0.1 98,317 + +0.1 98,318 - +0.1 98,318 - +0.0 98,318 - -0.6 98,318 - +0.7 98,318 - -1.0 98,318 - +0.5 98,382 + -1.2 98,399 + +0.1 98,399 + -0.5 98,400 - -1.0 98,400 - -1.6 98,400 - +1.5 98,588 + -0.5 98,588 + -0.8 98,742 - Psyr_0090 0.20 -0.6 98,742 - Psyr_0090 0.20 -0.3 98,742 - Psyr_0090 0.20 +0.9 98,873 + Psyr_0090 0.30 +0.0 98,873 + Psyr_0090 0.30 -0.0 98,873 + Psyr_0090 0.30 -0.5 98,873 + Psyr_0090 0.30 +1.7 98,873 + Psyr_0090 0.30 +0.8 98,873 + Psyr_0090 0.30 +1.3 98,873 + Psyr_0090 0.30 +0.6 98,873 + Psyr_0090 0.30 -0.5 98,873 + Psyr_0090 0.30 -0.7 98,874 - Psyr_0090 0.30 -0.6 98,874 - Psyr_0090 0.30 -0.9 98,874 - Psyr_0090 0.30 -0.1 98,874 - Psyr_0090 0.30 -0.3 98,874 - Psyr_0090 0.30 +0.1 98,874 - Psyr_0090 0.30 +0.9 98,874 - Psyr_0090 0.30 -1.0 98,874 - Psyr_0090 0.30 +0.8 98,912 + Psyr_0090 0.33 +1.1 98,912 + Psyr_0090 0.33 -0.1 98,912 + Psyr_0090 0.33 +1.7 98,912 + Psyr_0090 0.33 +0.9 98,912 + Psyr_0090 0.33 -0.5 98,913 - Psyr_0090 0.33 -1.3 98,913 - Psyr_0090 0.33 +0.7 98,913 - Psyr_0090 0.33 -0.6 98,913 - Psyr_0090 0.33 +4.1 98,913 - Psyr_0090 0.33 -0.1 98,973 + Psyr_0090 0.38 +1.1 98,974 - Psyr_0090 0.38 +0.4 99,122 + Psyr_0090 0.49 +0.0 99,122 + Psyr_0090 0.49 -0.3 99,122 + Psyr_0090 0.49 -0.1 99,122 + Psyr_0090 0.49 -0.2 99,122 + Psyr_0090 0.49 +0.0 99,122 + Psyr_0090 0.49 +1.8 99,122 + Psyr_0090 0.49 +0.1 99,122 + Psyr_0090 0.49 -0.1 99,123 - Psyr_0090 0.50 -0.5 99,123 - Psyr_0090 0.50 -1.4 99,123 - Psyr_0090 0.50 -0.9 99,123 - Psyr_0090 0.50 -0.8 99,123 - Psyr_0090 0.50 +0.6 99,123 - Psyr_0090 0.50 +0.2 99,123 - Psyr_0090 0.50 -0.5 99,123 - Psyr_0090 0.50 +1.7 99,123 - Psyr_0090 0.50 -2.2 99,123 - Psyr_0090 0.50 +0.3 99,123 - Psyr_0090 0.50 -0.3 99,123 - Psyr_0090 0.50 -0.3 99,123 - Psyr_0090 0.50 -0.2 99,123 - Psyr_0090 0.50 -0.6 99,271 - Psyr_0090 0.61 -0.1 99,354 - Psyr_0090 0.68 -0.3 99,509 + Psyr_0090 0.80 +0.1 99,509 + Psyr_0090 0.80 +0.3 99,510 - Psyr_0090 0.80 +0.1 99,510 - Psyr_0090 0.80 +0.3 99,510 - Psyr_0090 0.80 +0.3 99,510 - Psyr_0090 0.80 -1.4 99,510 - Psyr_0090 0.80 -0.7 99,510 - Psyr_0090 0.80 -1.4 99,510 - Psyr_0090 0.80 -1.5 99,519 - Psyr_0090 0.80 +0.9 99,519 - Psyr_0090 0.80 -0.1 99,519 - Psyr_0090 0.80 +0.8 99,770 + +0.8 99,770 + +0.3 99,770 + +0.0 99,770 + +1.0 99,770 + -0.1 99,770 + +0.3 99,770 + +0.4 99,770 + -0.3 99,770 + +1.2 99,770 + +0.1 99,770 + -0.5 99,770 + +0.9 99,770 + +0.9 99,770 + -0.1 99,770 + +0.2 99,770 + +0.3 99,770 + +0.2 99,770 + -0.5 99,770 + -1.1 99,770 + -0.5 99,771 - -0.0 99,771 - -0.7 99,771 - -0.6 99,771 - +0.3 99,771 - +0.4 99,771 - +2.4 99,771 - -0.7 99,771 - -0.5 99,771 - -0.1 99,771 - -0.5 99,771 - +0.0 99,771 - -0.0 99,771 - +0.3 99,771 - +0.8 99,771 - +0.9 99,771 - -0.5 99,840 + +2.0 99,840 + -2.1 99,840 + -0.5 99,840 + -0.0 99,841 - +3.4 99,841 - +0.5 99,841 - -0.1 99,841 - +0.2 99,841 - -0.3 99,967 + +0.3 99,967 + +1.5 99,968 - -0.4 99,968 - -0.9 99,968 - -0.9 99,968 - +0.2 99,968 - -0.0 99,968 - +0.9 99,983 - -0.2 99,997 + +1.4 99,997 + -0.6 99,997 + -0.0 100,094 - Psyr_0091 0.10 +0.6 100,141 + Psyr_0091 0.13 +1.1 100,147 + Psyr_0091 0.14 +0.6 100,147 + Psyr_0091 0.14 -0.9 100,148 - Psyr_0091 0.14 -0.4 100,266 - Psyr_0091 0.22 +0.9 100,486 + Psyr_0091 0.37 -1.0 100,486 + Psyr_0091 0.37 +2.5 100,487 - Psyr_0091 0.37 +1.0 100,487 - Psyr_0091 0.37 -0.5 100,487 - Psyr_0091 0.37 -0.9 100,504 + Psyr_0091 0.39 -0.7 100,504 + Psyr_0091 0.39 +0.0 100,504 + Psyr_0091 0.39 -1.1 100,504 + Psyr_0091 0.39 -0.2 100,505 - Psyr_0091 0.39 -0.3 100,505 - Psyr_0091 0.39 -1.7 100,505 - Psyr_0091 0.39 -1.0 100,505 - Psyr_0091 0.39 +0.7 100,505 - Psyr_0091 0.39 +0.8 100,505 - Psyr_0091 0.39 -0.5 100,505 - Psyr_0091 0.39 -0.9 100,505 - Psyr_0091 0.39 +0.2 100,505 - Psyr_0091 0.39 +1.1 100,547 - Psyr_0091 0.42 +0.9
Or see this region's nucleotide sequence