Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37380 and RR42_RS37385 are separated by 20 nucleotides RR42_RS37385 and RR42_RS37390 overlap by 1 nucleotides RR42_RS37390 and RR42_RS37395 are separated by 13 nucleotides
RR42_RS37380: RR42_RS37380 - 2-hydroxyacid dehydrogenase, at 3,864,413 to 3,865,348
_RS37380
RR42_RS37385: RR42_RS37385 - membrane protein, at 3,865,369 to 3,866,742
_RS37385
RR42_RS37390: RR42_RS37390 - XRE family transcriptional regulator, at 3,866,742 to 3,867,440
_RS37390
RR42_RS37395: RR42_RS37395 - glucokinase, at 3,867,454 to 3,868,491
_RS37395
Position (kb)
3865
3866
3867 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3864.480 kb on - strand at 3864.480 kb on - strand at 3864.480 kb on - strand at 3864.641 kb on + strand, within RR42_RS37380 at 3864.953 kb on + strand, within RR42_RS37380 at 3864.960 kb on - strand, within RR42_RS37380 at 3864.998 kb on + strand, within RR42_RS37380 at 3864.998 kb on + strand, within RR42_RS37380 at 3864.999 kb on - strand, within RR42_RS37380 at 3864.999 kb on - strand, within RR42_RS37380 at 3864.999 kb on - strand, within RR42_RS37380 at 3865.176 kb on + strand, within RR42_RS37380 at 3865.176 kb on + strand, within RR42_RS37380 at 3865.177 kb on - strand, within RR42_RS37380 at 3865.177 kb on - strand, within RR42_RS37380 at 3865.177 kb on - strand, within RR42_RS37380 at 3865.178 kb on + strand, within RR42_RS37380 at 3865.512 kb on - strand, within RR42_RS37385 at 3865.512 kb on - strand, within RR42_RS37385 at 3865.606 kb on + strand, within RR42_RS37385 at 3865.606 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.668 kb on - strand, within RR42_RS37385 at 3865.668 kb on - strand, within RR42_RS37385 at 3865.668 kb on - strand, within RR42_RS37385 at 3865.965 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.364 kb on - strand, within RR42_RS37385 at 3866.364 kb on - strand, within RR42_RS37385 at 3866.364 kb on - strand, within RR42_RS37385 at 3866.450 kb on + strand, within RR42_RS37385 at 3866.451 kb on - strand, within RR42_RS37385 at 3866.479 kb on - strand, within RR42_RS37385 at 3866.479 kb on - strand, within RR42_RS37385 at 3866.481 kb on - strand, within RR42_RS37385 at 3866.622 kb on - strand at 3866.661 kb on - strand at 3866.661 kb on - strand at 3866.779 kb on + strand at 3866.780 kb on - strand at 3866.780 kb on - strand at 3866.795 kb on + strand at 3866.842 kb on + strand, within RR42_RS37390 at 3866.843 kb on - strand, within RR42_RS37390 at 3866.884 kb on + strand, within RR42_RS37390 at 3867.127 kb on + strand, within RR42_RS37390 at 3867.286 kb on + strand, within RR42_RS37390 at 3867.286 kb on + strand, within RR42_RS37390 at 3867.286 kb on + strand, within RR42_RS37390 at 3867.287 kb on - strand, within RR42_RS37390 at 3867.287 kb on - strand, within RR42_RS37390 at 3867.393 kb on + strand at 3867.394 kb on - strand at 3867.465 kb on + strand at 3867.465 kb on + strand at 3867.465 kb on + strand at 3867.466 kb on - strand at 3867.466 kb on - strand at 3867.466 kb on - strand at 3867.588 kb on - strand, within RR42_RS37395 at 3867.588 kb on - strand, within RR42_RS37395 at 3867.609 kb on - strand, within RR42_RS37395 at 3867.609 kb on - strand, within RR42_RS37395 at 3867.609 kb on - strand, within RR42_RS37395 at 3867.701 kb on + strand, within RR42_RS37395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 3,864,480 - -0.3 3,864,480 - -1.6 3,864,480 - +0.0 3,864,641 + RR42_RS37380 0.24 +0.1 3,864,953 + RR42_RS37380 0.58 +1.4 3,864,960 - RR42_RS37380 0.58 -0.5 3,864,998 + RR42_RS37380 0.62 -0.6 3,864,998 + RR42_RS37380 0.62 +0.2 3,864,999 - RR42_RS37380 0.63 +2.0 3,864,999 - RR42_RS37380 0.63 +0.1 3,864,999 - RR42_RS37380 0.63 -0.6 3,865,176 + RR42_RS37380 0.82 -0.8 3,865,176 + RR42_RS37380 0.82 -0.7 3,865,177 - RR42_RS37380 0.82 -0.2 3,865,177 - RR42_RS37380 0.82 +0.4 3,865,177 - RR42_RS37380 0.82 -2.3 3,865,178 + RR42_RS37380 0.82 +0.2 3,865,512 - RR42_RS37385 0.10 +0.0 3,865,512 - RR42_RS37385 0.10 -0.6 3,865,606 + RR42_RS37385 0.17 -0.1 3,865,606 + RR42_RS37385 0.17 +0.2 3,865,667 + RR42_RS37385 0.22 -0.3 3,865,667 + RR42_RS37385 0.22 -0.8 3,865,667 + RR42_RS37385 0.22 -0.3 3,865,667 + RR42_RS37385 0.22 +0.2 3,865,668 - RR42_RS37385 0.22 -2.4 3,865,668 - RR42_RS37385 0.22 -0.6 3,865,668 - RR42_RS37385 0.22 -0.5 3,865,965 + RR42_RS37385 0.43 +0.7 3,866,363 + RR42_RS37385 0.72 -0.6 3,866,363 + RR42_RS37385 0.72 +0.3 3,866,363 + RR42_RS37385 0.72 +1.0 3,866,363 + RR42_RS37385 0.72 -1.6 3,866,364 - RR42_RS37385 0.72 -0.2 3,866,364 - RR42_RS37385 0.72 -1.9 3,866,364 - RR42_RS37385 0.72 -1.6 3,866,450 + RR42_RS37385 0.79 -0.4 3,866,451 - RR42_RS37385 0.79 -1.2 3,866,479 - RR42_RS37385 0.81 -0.3 3,866,479 - RR42_RS37385 0.81 +0.5 3,866,481 - RR42_RS37385 0.81 -0.0 3,866,622 - +0.6 3,866,661 - +0.2 3,866,661 - -0.5 3,866,779 + -0.2 3,866,780 - +0.1 3,866,780 - +0.7 3,866,795 + -0.1 3,866,842 + RR42_RS37390 0.14 +1.6 3,866,843 - RR42_RS37390 0.14 -1.0 3,866,884 + RR42_RS37390 0.20 -1.6 3,867,127 + RR42_RS37390 0.55 -1.4 3,867,286 + RR42_RS37390 0.78 -1.2 3,867,286 + RR42_RS37390 0.78 +1.0 3,867,286 + RR42_RS37390 0.78 +1.4 3,867,287 - RR42_RS37390 0.78 +0.2 3,867,287 - RR42_RS37390 0.78 -1.0 3,867,393 + -0.7 3,867,394 - -0.7 3,867,465 + -0.4 3,867,465 + -0.6 3,867,465 + -0.6 3,867,466 - -0.2 3,867,466 - -0.9 3,867,466 - -0.1 3,867,588 - RR42_RS37395 0.13 -0.3 3,867,588 - RR42_RS37395 0.13 -0.4 3,867,609 - RR42_RS37395 0.15 -0.2 3,867,609 - RR42_RS37395 0.15 -2.0 3,867,609 - RR42_RS37395 0.15 +0.3 3,867,701 + RR42_RS37395 0.24 -0.1
Or see this region's nucleotide sequence