Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33725 and RR42_RS33730 are separated by 190 nucleotides RR42_RS33730 and RR42_RS33735 are separated by 43 nucleotides
RR42_RS33725: RR42_RS33725 - transketolase, at 2,993,059 to 2,994,060
_RS33725
RR42_RS33730: RR42_RS33730 - hypothetical protein, at 2,994,251 to 2,995,537
_RS33730
RR42_RS33735: RR42_RS33735 - pyruvate kinase, at 2,995,581 to 2,997,011
_RS33735
Position (kb)
2994
2995
2996 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2993.267 kb on - strand, within RR42_RS33725 at 2993.269 kb on + strand, within RR42_RS33725 at 2993.270 kb on - strand, within RR42_RS33725 at 2993.353 kb on + strand, within RR42_RS33725 at 2993.353 kb on + strand, within RR42_RS33725 at 2993.353 kb on + strand, within RR42_RS33725 at 2993.354 kb on - strand, within RR42_RS33725 at 2993.354 kb on - strand, within RR42_RS33725 at 2993.354 kb on - strand, within RR42_RS33725 at 2993.596 kb on + strand, within RR42_RS33725 at 2993.597 kb on - strand, within RR42_RS33725 at 2993.641 kb on + strand, within RR42_RS33725 at 2993.641 kb on + strand, within RR42_RS33725 at 2993.642 kb on - strand, within RR42_RS33725 at 2994.053 kb on + strand at 2994.112 kb on + strand at 2994.113 kb on - strand at 2994.288 kb on + strand at 2994.288 kb on + strand at 2994.289 kb on - strand at 2994.289 kb on - strand at 2994.326 kb on + strand at 2994.337 kb on - strand at 2994.337 kb on - strand at 2994.449 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.778 kb on - strand, within RR42_RS33730 at 2994.778 kb on - strand, within RR42_RS33730 at 2994.984 kb on - strand, within RR42_RS33730 at 2995.147 kb on - strand, within RR42_RS33730 at 2995.437 kb on + strand at 2995.438 kb on - strand at 2995.502 kb on + strand at 2995.503 kb on - strand at 2995.717 kb on + strand at 2995.718 kb on - strand at 2995.882 kb on + strand, within RR42_RS33735 at 2995.899 kb on + strand, within RR42_RS33735 at 2995.997 kb on - strand, within RR42_RS33735 at 2995.997 kb on - strand, within RR42_RS33735 at 2996.110 kb on + strand, within RR42_RS33735 at 2996.110 kb on + strand, within RR42_RS33735 at 2996.111 kb on - strand, within RR42_RS33735 at 2996.468 kb on - strand, within RR42_RS33735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,993,267 - RR42_RS33725 0.21 -0.3 2,993,269 + RR42_RS33725 0.21 +0.5 2,993,270 - RR42_RS33725 0.21 -0.2 2,993,353 + RR42_RS33725 0.29 -1.0 2,993,353 + RR42_RS33725 0.29 -1.2 2,993,353 + RR42_RS33725 0.29 +0.6 2,993,354 - RR42_RS33725 0.29 +0.7 2,993,354 - RR42_RS33725 0.29 -1.7 2,993,354 - RR42_RS33725 0.29 -0.1 2,993,596 + RR42_RS33725 0.54 +0.4 2,993,597 - RR42_RS33725 0.54 -0.4 2,993,641 + RR42_RS33725 0.58 -0.6 2,993,641 + RR42_RS33725 0.58 -0.1 2,993,642 - RR42_RS33725 0.58 -1.0 2,994,053 + +0.5 2,994,112 + +0.0 2,994,113 - -0.9 2,994,288 + -1.0 2,994,288 + -0.1 2,994,289 - -1.0 2,994,289 - -0.8 2,994,326 + +0.0 2,994,337 - -0.7 2,994,337 - -0.3 2,994,449 + RR42_RS33730 0.15 -0.3 2,994,777 + RR42_RS33730 0.41 -1.5 2,994,777 + RR42_RS33730 0.41 +0.7 2,994,777 + RR42_RS33730 0.41 -0.0 2,994,778 - RR42_RS33730 0.41 +0.0 2,994,778 - RR42_RS33730 0.41 +0.3 2,994,984 - RR42_RS33730 0.57 -1.2 2,995,147 - RR42_RS33730 0.70 -2.1 2,995,437 + +0.7 2,995,438 - -1.1 2,995,502 + -1.1 2,995,503 - -1.8 2,995,717 + -1.3 2,995,718 - +0.3 2,995,882 + RR42_RS33735 0.21 +1.4 2,995,899 + RR42_RS33735 0.22 -0.5 2,995,997 - RR42_RS33735 0.29 -0.3 2,995,997 - RR42_RS33735 0.29 +0.7 2,996,110 + RR42_RS33735 0.37 +1.9 2,996,110 + RR42_RS33735 0.37 -0.3 2,996,111 - RR42_RS33735 0.37 +0.2 2,996,468 - RR42_RS33735 0.62 -1.0
Or see this region's nucleotide sequence