Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33270 and RR42_RS33275 are separated by 71 nucleotides RR42_RS33275 and RR42_RS33280 are separated by 450 nucleotides RR42_RS33280 and RR42_RS33285 are separated by 28 nucleotides RR42_RS33285 and RR42_RS33290 are separated by 326 nucleotides
RR42_RS33270: RR42_RS33270 - hypothetical protein, at 2,880,027 to 2,880,512
_RS33270
RR42_RS33275: RR42_RS33275 - hypothetical protein, at 2,880,584 to 2,880,874
_RS33275
RR42_RS33280: RR42_RS33280 - diguanylate cyclase, at 2,881,325 to 2,882,440
_RS33280
RR42_RS33285: RR42_RS33285 - membrane protein, at 2,882,469 to 2,882,930
_RS33285
RR42_RS33290: RR42_RS33290 - histidine kinase, at 2,883,257 to 2,885,233
_RS33290
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2880.464 kb on + strand at 2880.464 kb on + strand at 2880.465 kb on - strand at 2880.515 kb on + strand at 2880.516 kb on - strand at 2880.516 kb on - strand at 2880.516 kb on - strand at 2880.641 kb on + strand, within RR42_RS33275 at 2880.642 kb on - strand, within RR42_RS33275 at 2880.642 kb on - strand, within RR42_RS33275 at 2880.642 kb on - strand, within RR42_RS33275 at 2880.642 kb on - strand, within RR42_RS33275 at 2880.747 kb on - strand, within RR42_RS33275 at 2880.753 kb on + strand, within RR42_RS33275 at 2880.753 kb on + strand, within RR42_RS33275 at 2880.753 kb on + strand, within RR42_RS33275 at 2880.754 kb on - strand, within RR42_RS33275 at 2880.754 kb on - strand, within RR42_RS33275 at 2880.754 kb on - strand, within RR42_RS33275 at 2880.754 kb on - strand, within RR42_RS33275 at 2880.805 kb on + strand, within RR42_RS33275 at 2880.806 kb on - strand, within RR42_RS33275 at 2880.904 kb on + strand at 2880.923 kb on + strand at 2880.984 kb on + strand at 2880.984 kb on + strand at 2880.984 kb on + strand at 2880.985 kb on - strand at 2880.985 kb on - strand at 2881.078 kb on + strand at 2881.078 kb on + strand at 2881.079 kb on - strand at 2881.080 kb on + strand at 2881.080 kb on + strand at 2881.081 kb on - strand at 2881.088 kb on + strand at 2881.088 kb on + strand at 2881.088 kb on + strand at 2881.088 kb on + strand at 2881.088 kb on + strand at 2881.089 kb on - strand at 2881.089 kb on - strand at 2881.089 kb on - strand at 2881.089 kb on - strand at 2881.089 kb on - strand at 2881.089 kb on - strand at 2881.113 kb on - strand at 2881.240 kb on + strand at 2881.240 kb on + strand at 2881.241 kb on - strand at 2881.362 kb on - strand at 2881.362 kb on - strand at 2881.365 kb on - strand at 2881.429 kb on + strand at 2881.544 kb on + strand, within RR42_RS33280 at 2881.613 kb on + strand, within RR42_RS33280 at 2881.614 kb on - strand, within RR42_RS33280 at 2881.614 kb on - strand, within RR42_RS33280 at 2881.634 kb on + strand, within RR42_RS33280 at 2881.634 kb on + strand, within RR42_RS33280 at 2881.634 kb on + strand, within RR42_RS33280 at 2881.634 kb on + strand, within RR42_RS33280 at 2881.634 kb on + strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.635 kb on - strand, within RR42_RS33280 at 2881.679 kb on + strand, within RR42_RS33280 at 2881.679 kb on + strand, within RR42_RS33280 at 2881.679 kb on + strand, within RR42_RS33280 at 2881.680 kb on - strand, within RR42_RS33280 at 2881.680 kb on - strand, within RR42_RS33280 at 2881.733 kb on + strand, within RR42_RS33280 at 2881.733 kb on + strand, within RR42_RS33280 at 2881.733 kb on + strand, within RR42_RS33280 at 2881.733 kb on + strand, within RR42_RS33280 at 2881.734 kb on - strand, within RR42_RS33280 at 2881.734 kb on - strand, within RR42_RS33280 at 2881.734 kb on - strand, within RR42_RS33280 at 2881.734 kb on - strand, within RR42_RS33280 at 2881.734 kb on - strand, within RR42_RS33280 at 2881.734 kb on - strand, within RR42_RS33280 at 2881.856 kb on + strand, within RR42_RS33280 at 2881.856 kb on + strand, within RR42_RS33280 at 2881.857 kb on - strand, within RR42_RS33280 at 2881.995 kb on - strand, within RR42_RS33280 at 2881.995 kb on - strand, within RR42_RS33280 at 2882.172 kb on - strand, within RR42_RS33280 at 2882.456 kb on + strand at 2882.457 kb on - strand at 2882.626 kb on + strand, within RR42_RS33285 at 2882.627 kb on - strand, within RR42_RS33285 at 2882.632 kb on + strand, within RR42_RS33285 at 2882.633 kb on - strand, within RR42_RS33285 at 2882.707 kb on - strand, within RR42_RS33285 at 2882.854 kb on + strand, within RR42_RS33285 at 2883.088 kb on - strand at 2883.131 kb on + strand at 2883.132 kb on - strand at 2883.162 kb on + strand at 2883.162 kb on + strand at 2883.162 kb on + strand at 2883.163 kb on - strand at 2883.194 kb on + strand at 2883.270 kb on + strand at 2883.270 kb on + strand at 2883.271 kb on - strand at 2883.344 kb on + strand at 2883.348 kb on - strand at 2883.348 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,880,464 + -0.5 2,880,464 + +0.7 2,880,465 - +1.1 2,880,515 + +0.8 2,880,516 - -0.3 2,880,516 - +0.5 2,880,516 - -0.3 2,880,641 + RR42_RS33275 0.20 -0.9 2,880,642 - RR42_RS33275 0.20 +0.2 2,880,642 - RR42_RS33275 0.20 +1.1 2,880,642 - RR42_RS33275 0.20 -1.1 2,880,642 - RR42_RS33275 0.20 -1.1 2,880,747 - RR42_RS33275 0.56 -0.3 2,880,753 + RR42_RS33275 0.58 -0.5 2,880,753 + RR42_RS33275 0.58 -0.6 2,880,753 + RR42_RS33275 0.58 +0.1 2,880,754 - RR42_RS33275 0.58 -0.0 2,880,754 - RR42_RS33275 0.58 -0.6 2,880,754 - RR42_RS33275 0.58 -0.1 2,880,754 - RR42_RS33275 0.58 -0.0 2,880,805 + RR42_RS33275 0.76 -0.4 2,880,806 - RR42_RS33275 0.76 -0.5 2,880,904 + +0.4 2,880,923 + -0.9 2,880,984 + -0.0 2,880,984 + +0.2 2,880,984 + +0.1 2,880,985 - +1.1 2,880,985 - +0.3 2,881,078 + -0.0 2,881,078 + -2.4 2,881,079 - +0.3 2,881,080 + +0.3 2,881,080 + +0.5 2,881,081 - +0.2 2,881,088 + +2.8 2,881,088 + +0.1 2,881,088 + -0.9 2,881,088 + -0.2 2,881,088 + -0.3 2,881,089 - -0.5 2,881,089 - +0.0 2,881,089 - +0.7 2,881,089 - +0.8 2,881,089 - -0.1 2,881,089 - -0.6 2,881,113 - -0.3 2,881,240 + -2.1 2,881,240 + +1.3 2,881,241 - -0.8 2,881,362 - -0.4 2,881,362 - -0.4 2,881,365 - -0.6 2,881,429 + +0.0 2,881,544 + RR42_RS33280 0.20 +1.1 2,881,613 + RR42_RS33280 0.26 -0.7 2,881,614 - RR42_RS33280 0.26 +2.0 2,881,614 - RR42_RS33280 0.26 +1.0 2,881,634 + RR42_RS33280 0.28 -0.7 2,881,634 + RR42_RS33280 0.28 -0.0 2,881,634 + RR42_RS33280 0.28 +0.4 2,881,634 + RR42_RS33280 0.28 -0.1 2,881,634 + RR42_RS33280 0.28 +0.2 2,881,635 - RR42_RS33280 0.28 -1.8 2,881,635 - RR42_RS33280 0.28 +0.8 2,881,635 - RR42_RS33280 0.28 +0.7 2,881,635 - RR42_RS33280 0.28 -0.3 2,881,635 - RR42_RS33280 0.28 -0.7 2,881,635 - RR42_RS33280 0.28 +0.4 2,881,635 - RR42_RS33280 0.28 -0.1 2,881,679 + RR42_RS33280 0.32 -2.5 2,881,679 + RR42_RS33280 0.32 -0.5 2,881,679 + RR42_RS33280 0.32 +0.3 2,881,680 - RR42_RS33280 0.32 +2.0 2,881,680 - RR42_RS33280 0.32 -0.4 2,881,733 + RR42_RS33280 0.37 -0.1 2,881,733 + RR42_RS33280 0.37 -0.3 2,881,733 + RR42_RS33280 0.37 -0.2 2,881,733 + RR42_RS33280 0.37 -0.8 2,881,734 - RR42_RS33280 0.37 +0.4 2,881,734 - RR42_RS33280 0.37 -0.4 2,881,734 - RR42_RS33280 0.37 +0.5 2,881,734 - RR42_RS33280 0.37 -0.6 2,881,734 - RR42_RS33280 0.37 -0.9 2,881,734 - RR42_RS33280 0.37 +0.4 2,881,856 + RR42_RS33280 0.48 +0.7 2,881,856 + RR42_RS33280 0.48 +0.1 2,881,857 - RR42_RS33280 0.48 +1.0 2,881,995 - RR42_RS33280 0.60 +0.1 2,881,995 - RR42_RS33280 0.60 -0.5 2,882,172 - RR42_RS33280 0.76 +0.4 2,882,456 + -0.0 2,882,457 - -1.5 2,882,626 + RR42_RS33285 0.34 -0.8 2,882,627 - RR42_RS33285 0.34 +0.5 2,882,632 + RR42_RS33285 0.35 -0.6 2,882,633 - RR42_RS33285 0.35 -0.1 2,882,707 - RR42_RS33285 0.52 +0.7 2,882,854 + RR42_RS33285 0.83 -0.0 2,883,088 - -1.2 2,883,131 + +0.1 2,883,132 - -0.2 2,883,162 + -1.9 2,883,162 + -0.3 2,883,162 + -0.2 2,883,163 - +0.4 2,883,194 + -1.6 2,883,270 + +0.4 2,883,270 + -0.9 2,883,271 - +0.6 2,883,344 + +0.4 2,883,348 - -0.9 2,883,348 - -0.8
Or see this region's nucleotide sequence