Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31905 and RR42_RS31910 are separated by 11 nucleotides RR42_RS31910 and RR42_RS31915 are separated by 14 nucleotides RR42_RS31915 and RR42_RS31920 are separated by 50 nucleotides
RR42_RS31905: RR42_RS31905 - acyl-CoA dehydrogenase, at 2,535,045 to 2,536,271
_RS31905
RR42_RS31910: RR42_RS31910 - acyl-CoA dehydrogenase, at 2,536,283 to 2,537,527
_RS31910
RR42_RS31915: RR42_RS31915 - short-chain dehydrogenase, at 2,537,542 to 2,538,417
_RS31915
RR42_RS31920: RR42_RS31920 - acetyl-CoA acetyltransferase, at 2,538,468 to 2,539,619
_RS31920
Position (kb)
2536
2537
2538 Strain fitness (log2 ratio)
-2
-1
0
1 at 2535.367 kb on + strand, within RR42_RS31905 at 2535.368 kb on - strand, within RR42_RS31905 at 2535.981 kb on - strand, within RR42_RS31905 at 2536.284 kb on + strand at 2536.411 kb on - strand, within RR42_RS31910 at 2536.420 kb on + strand, within RR42_RS31910 at 2536.712 kb on - strand, within RR42_RS31910 at 2537.553 kb on - strand at 2537.553 kb on - strand at 2537.771 kb on + strand, within RR42_RS31915 at 2537.771 kb on + strand, within RR42_RS31915 at 2537.772 kb on - strand, within RR42_RS31915 at 2537.774 kb on + strand, within RR42_RS31915 at 2537.936 kb on + strand, within RR42_RS31915 at 2537.936 kb on + strand, within RR42_RS31915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,535,367 + RR42_RS31905 0.26 -0.4 2,535,368 - RR42_RS31905 0.26 -0.5 2,535,981 - RR42_RS31905 0.76 -0.1 2,536,284 + -1.3 2,536,411 - RR42_RS31910 0.10 +0.6 2,536,420 + RR42_RS31910 0.11 +0.7 2,536,712 - RR42_RS31910 0.34 +0.7 2,537,553 - -0.5 2,537,553 - -1.0 2,537,771 + RR42_RS31915 0.26 +0.2 2,537,771 + RR42_RS31915 0.26 -0.4 2,537,772 - RR42_RS31915 0.26 -1.9 2,537,774 + RR42_RS31915 0.26 -0.5 2,537,936 + RR42_RS31915 0.45 -0.2 2,537,936 + RR42_RS31915 0.45 +0.5
Or see this region's nucleotide sequence