Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31230 and RR42_RS31235 overlap by 4 nucleotides RR42_RS31235 and RR42_RS31240 overlap by 8 nucleotides
RR42_RS31230: RR42_RS31230 - acyltransferase, at 2,383,411 to 2,384,367
_RS31230
RR42_RS31235: RR42_RS31235 - glycosyl transferase, at 2,384,364 to 2,385,107
_RS31235
RR42_RS31240: RR42_RS31240 - AMP-dependent synthetase, at 2,385,100 to 2,386,818
_RS31240
Position (kb)
2384
2385
2386 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2383.421 kb on + strand at 2383.631 kb on + strand, within RR42_RS31230 at 2384.015 kb on + strand, within RR42_RS31230 at 2384.021 kb on + strand, within RR42_RS31230 at 2384.050 kb on + strand, within RR42_RS31230 at 2384.050 kb on + strand, within RR42_RS31230 at 2384.051 kb on - strand, within RR42_RS31230 at 2384.156 kb on + strand, within RR42_RS31230 at 2384.198 kb on + strand, within RR42_RS31230 at 2384.198 kb on + strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.199 kb on - strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.201 kb on + strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.202 kb on - strand, within RR42_RS31230 at 2384.255 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.264 kb on + strand, within RR42_RS31230 at 2384.284 kb on + strand at 2384.432 kb on + strand at 2384.432 kb on + strand at 2384.433 kb on - strand at 2384.497 kb on + strand, within RR42_RS31235 at 2384.497 kb on + strand, within RR42_RS31235 at 2384.498 kb on - strand, within RR42_RS31235 at 2384.515 kb on - strand, within RR42_RS31235 at 2384.629 kb on + strand, within RR42_RS31235 at 2384.630 kb on - strand, within RR42_RS31235 at 2384.705 kb on - strand, within RR42_RS31235 at 2384.855 kb on + strand, within RR42_RS31235 at 2385.077 kb on - strand at 2385.077 kb on - strand at 2385.101 kb on + strand at 2385.102 kb on - strand at 2385.102 kb on - strand at 2385.102 kb on - strand at 2385.251 kb on - strand at 2385.498 kb on + strand, within RR42_RS31240 at 2385.499 kb on - strand, within RR42_RS31240 at 2385.499 kb on - strand, within RR42_RS31240 at 2385.764 kb on - strand, within RR42_RS31240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,383,421 + -0.9 2,383,631 + RR42_RS31230 0.23 +0.1 2,384,015 + RR42_RS31230 0.63 +0.4 2,384,021 + RR42_RS31230 0.64 +0.2 2,384,050 + RR42_RS31230 0.67 -1.2 2,384,050 + RR42_RS31230 0.67 +1.9 2,384,051 - RR42_RS31230 0.67 -0.4 2,384,156 + RR42_RS31230 0.78 -0.1 2,384,198 + RR42_RS31230 0.82 +0.3 2,384,198 + RR42_RS31230 0.82 -0.3 2,384,199 - RR42_RS31230 0.82 +1.3 2,384,199 - RR42_RS31230 0.82 +0.6 2,384,199 - RR42_RS31230 0.82 +0.4 2,384,199 - RR42_RS31230 0.82 +0.2 2,384,199 - RR42_RS31230 0.82 +0.7 2,384,201 + RR42_RS31230 0.83 -0.8 2,384,201 + RR42_RS31230 0.83 -0.3 2,384,201 + RR42_RS31230 0.83 +0.0 2,384,202 - RR42_RS31230 0.83 -0.0 2,384,202 - RR42_RS31230 0.83 -0.7 2,384,202 - RR42_RS31230 0.83 -0.7 2,384,255 + RR42_RS31230 0.88 +0.8 2,384,264 + RR42_RS31230 0.89 -0.2 2,384,264 + RR42_RS31230 0.89 -1.0 2,384,264 + RR42_RS31230 0.89 +0.0 2,384,284 + +0.3 2,384,432 + +0.1 2,384,432 + +0.6 2,384,433 - +0.2 2,384,497 + RR42_RS31235 0.18 +0.7 2,384,497 + RR42_RS31235 0.18 +0.8 2,384,498 - RR42_RS31235 0.18 -1.4 2,384,515 - RR42_RS31235 0.20 -0.6 2,384,629 + RR42_RS31235 0.36 -2.1 2,384,630 - RR42_RS31235 0.36 -0.4 2,384,705 - RR42_RS31235 0.46 -0.6 2,384,855 + RR42_RS31235 0.66 -0.7 2,385,077 - +0.9 2,385,077 - +0.6 2,385,101 + +0.2 2,385,102 - +0.5 2,385,102 - -1.1 2,385,102 - +0.6 2,385,251 - -0.3 2,385,498 + RR42_RS31240 0.23 -0.2 2,385,499 - RR42_RS31240 0.23 -0.2 2,385,499 - RR42_RS31240 0.23 +0.9 2,385,764 - RR42_RS31240 0.39 +0.6
Or see this region's nucleotide sequence