Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29500 and RR42_RS29505 are separated by 19 nucleotides RR42_RS29505 and RR42_RS29510 are separated by 138 nucleotides
RR42_RS29500: RR42_RS29500 - acyl-CoA dehydrogenase, at 1,975,606 to 1,976,781
_RS29500
RR42_RS29505: RR42_RS29505 - hypothetical protein, at 1,976,801 to 1,977,181
_RS29505
RR42_RS29510: RR42_RS29510 - hypothetical protein, at 1,977,320 to 1,978,909
_RS29510
Position (kb)
1976
1977
1978 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1975.831 kb on + strand, within RR42_RS29500 at 1975.831 kb on + strand, within RR42_RS29500 at 1975.831 kb on + strand, within RR42_RS29500 at 1975.832 kb on - strand, within RR42_RS29500 at 1975.832 kb on - strand, within RR42_RS29500 at 1975.832 kb on - strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.071 kb on + strand, within RR42_RS29500 at 1976.072 kb on - strand, within RR42_RS29500 at 1976.072 kb on - strand, within RR42_RS29500 at 1976.380 kb on + strand, within RR42_RS29500 at 1976.380 kb on + strand, within RR42_RS29500 at 1976.381 kb on - strand, within RR42_RS29500 at 1976.381 kb on - strand, within RR42_RS29500 at 1976.386 kb on + strand, within RR42_RS29500 at 1976.386 kb on + strand, within RR42_RS29500 at 1976.387 kb on - strand, within RR42_RS29500 at 1976.387 kb on - strand, within RR42_RS29500 at 1976.527 kb on + strand, within RR42_RS29500 at 1976.528 kb on - strand, within RR42_RS29500 at 1976.539 kb on + strand, within RR42_RS29500 at 1976.698 kb on + strand at 1976.699 kb on - strand at 1976.825 kb on + strand at 1976.826 kb on - strand at 1976.826 kb on - strand at 1976.826 kb on - strand at 1976.826 kb on - strand at 1976.826 kb on - strand at 1977.002 kb on + strand, within RR42_RS29505 at 1977.002 kb on + strand, within RR42_RS29505 at 1977.003 kb on - strand, within RR42_RS29505 at 1977.151 kb on + strand at 1977.179 kb on + strand at 1977.179 kb on + strand at 1977.179 kb on + strand at 1977.179 kb on + strand at 1977.180 kb on - strand at 1977.180 kb on - strand at 1977.258 kb on + strand at 1977.258 kb on + strand at 1977.259 kb on - strand at 1977.260 kb on + strand at 1977.260 kb on + strand at 1977.261 kb on - strand at 1977.261 kb on - strand at 1977.329 kb on + strand at 1977.454 kb on - strand at 1977.527 kb on + strand, within RR42_RS29510 at 1977.527 kb on + strand, within RR42_RS29510 at 1977.527 kb on + strand, within RR42_RS29510 at 1977.527 kb on + strand, within RR42_RS29510 at 1977.527 kb on + strand, within RR42_RS29510 at 1977.527 kb on + strand, within RR42_RS29510 at 1977.528 kb on - strand, within RR42_RS29510 at 1977.528 kb on - strand, within RR42_RS29510 at 1977.528 kb on - strand, within RR42_RS29510 at 1977.528 kb on - strand, within RR42_RS29510 at 1977.528 kb on - strand, within RR42_RS29510 at 1977.528 kb on - strand, within RR42_RS29510 at 1977.615 kb on + strand, within RR42_RS29510 at 1977.615 kb on + strand, within RR42_RS29510 at 1977.616 kb on - strand, within RR42_RS29510 at 1977.634 kb on - strand, within RR42_RS29510 at 1977.637 kb on + strand, within RR42_RS29510 at 1977.638 kb on - strand, within RR42_RS29510 at 1977.752 kb on + strand, within RR42_RS29510 at 1977.770 kb on + strand, within RR42_RS29510 at 1977.770 kb on + strand, within RR42_RS29510 at 1977.771 kb on - strand, within RR42_RS29510 at 1977.776 kb on + strand, within RR42_RS29510 at 1977.776 kb on + strand, within RR42_RS29510 at 1977.776 kb on + strand, within RR42_RS29510 at 1977.777 kb on - strand, within RR42_RS29510 at 1977.777 kb on - strand, within RR42_RS29510 at 1977.902 kb on + strand, within RR42_RS29510 at 1977.903 kb on - strand, within RR42_RS29510 at 1977.981 kb on + strand, within RR42_RS29510 at 1978.128 kb on + strand, within RR42_RS29510 at 1978.129 kb on - strand, within RR42_RS29510 at 1978.129 kb on - strand, within RR42_RS29510 at 1978.129 kb on - strand, within RR42_RS29510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 1,975,831 + RR42_RS29500 0.19 +0.5 1,975,831 + RR42_RS29500 0.19 -0.4 1,975,831 + RR42_RS29500 0.19 -0.5 1,975,832 - RR42_RS29500 0.19 -1.1 1,975,832 - RR42_RS29500 0.19 -0.2 1,975,832 - RR42_RS29500 0.19 +0.1 1,976,071 + RR42_RS29500 0.40 -0.3 1,976,071 + RR42_RS29500 0.40 -0.0 1,976,071 + RR42_RS29500 0.40 +0.4 1,976,071 + RR42_RS29500 0.40 +0.5 1,976,072 - RR42_RS29500 0.40 +2.4 1,976,072 - RR42_RS29500 0.40 -0.5 1,976,380 + RR42_RS29500 0.66 -0.8 1,976,380 + RR42_RS29500 0.66 -0.6 1,976,381 - RR42_RS29500 0.66 +1.1 1,976,381 - RR42_RS29500 0.66 +1.7 1,976,386 + RR42_RS29500 0.66 -0.8 1,976,386 + RR42_RS29500 0.66 -1.7 1,976,387 - RR42_RS29500 0.66 -0.0 1,976,387 - RR42_RS29500 0.66 -0.6 1,976,527 + RR42_RS29500 0.78 +0.5 1,976,528 - RR42_RS29500 0.78 +0.0 1,976,539 + RR42_RS29500 0.79 -0.2 1,976,698 + +0.7 1,976,699 - -0.5 1,976,825 + +0.0 1,976,826 - +0.1 1,976,826 - -0.1 1,976,826 - +0.1 1,976,826 - +0.6 1,976,826 - +0.0 1,977,002 + RR42_RS29505 0.53 -2.7 1,977,002 + RR42_RS29505 0.53 -0.3 1,977,003 - RR42_RS29505 0.53 -0.1 1,977,151 + +0.2 1,977,179 + +0.5 1,977,179 + +0.2 1,977,179 + +0.8 1,977,179 + -1.7 1,977,180 - +1.0 1,977,180 - -0.7 1,977,258 + +0.5 1,977,258 + -0.2 1,977,259 - +1.3 1,977,260 + -0.7 1,977,260 + +0.7 1,977,261 - -1.1 1,977,261 - +1.1 1,977,329 + -0.1 1,977,454 - -0.3 1,977,527 + RR42_RS29510 0.13 +0.4 1,977,527 + RR42_RS29510 0.13 -0.3 1,977,527 + RR42_RS29510 0.13 -0.5 1,977,527 + RR42_RS29510 0.13 +0.3 1,977,527 + RR42_RS29510 0.13 -0.2 1,977,527 + RR42_RS29510 0.13 +0.3 1,977,528 - RR42_RS29510 0.13 -0.2 1,977,528 - RR42_RS29510 0.13 +1.2 1,977,528 - RR42_RS29510 0.13 +1.4 1,977,528 - RR42_RS29510 0.13 +0.5 1,977,528 - RR42_RS29510 0.13 -0.5 1,977,528 - RR42_RS29510 0.13 +0.1 1,977,615 + RR42_RS29510 0.19 -0.2 1,977,615 + RR42_RS29510 0.19 -0.6 1,977,616 - RR42_RS29510 0.19 -1.2 1,977,634 - RR42_RS29510 0.20 +1.6 1,977,637 + RR42_RS29510 0.20 +0.2 1,977,638 - RR42_RS29510 0.20 -0.8 1,977,752 + RR42_RS29510 0.27 +0.2 1,977,770 + RR42_RS29510 0.28 +1.2 1,977,770 + RR42_RS29510 0.28 -2.1 1,977,771 - RR42_RS29510 0.28 +2.3 1,977,776 + RR42_RS29510 0.29 +0.4 1,977,776 + RR42_RS29510 0.29 -0.5 1,977,776 + RR42_RS29510 0.29 +1.2 1,977,777 - RR42_RS29510 0.29 -0.0 1,977,777 - RR42_RS29510 0.29 -1.1 1,977,902 + RR42_RS29510 0.37 +0.6 1,977,903 - RR42_RS29510 0.37 -1.0 1,977,981 + RR42_RS29510 0.42 -0.7 1,978,128 + RR42_RS29510 0.51 +0.3 1,978,129 - RR42_RS29510 0.51 +0.4 1,978,129 - RR42_RS29510 0.51 -0.1 1,978,129 - RR42_RS29510 0.51 +1.6
Or see this region's nucleotide sequence