Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24795 and RR42_RS24800 are separated by 28 nucleotides RR42_RS24800 and RR42_RS24805 are separated by 10 nucleotides
RR42_RS24795: RR42_RS24795 - butyryl-CoA dehydrogenase, at 901,264 to 902,412
_RS24795
RR42_RS24800: RR42_RS24800 - dehydratase, at 902,441 to 902,956
_RS24800
RR42_RS24805: RR42_RS24805 - thiolase, at 902,967 to 904,181
_RS24805
Position (kb) 902
903 Strain fitness (log2 ratio) -2
-1
0
1 at 901.594 kb on + strand, within RR42_RS24795 at 901.595 kb on - strand, within RR42_RS24795 at 902.018 kb on - strand, within RR42_RS24795 at 902.323 kb on + strand at 902.324 kb on - strand at 902.434 kb on + strand at 902.435 kb on - strand at 902.562 kb on - strand, within RR42_RS24800 at 902.562 kb on - strand, within RR42_RS24800 at 902.609 kb on + strand, within RR42_RS24800 at 902.660 kb on + strand, within RR42_RS24800 at 902.661 kb on - strand, within RR42_RS24800 at 902.711 kb on + strand, within RR42_RS24800 at 902.712 kb on - strand, within RR42_RS24800 at 902.712 kb on - strand, within RR42_RS24800 at 902.747 kb on + strand, within RR42_RS24800 at 902.747 kb on + strand, within RR42_RS24800 at 902.773 kb on - strand, within RR42_RS24800 at 903.013 kb on - strand at 903.013 kb on - strand at 903.405 kb on + strand, within RR42_RS24805 at 903.439 kb on - strand, within RR42_RS24805 at 903.632 kb on - strand, within RR42_RS24805 at 903.679 kb on - strand, within RR42_RS24805 at 903.714 kb on + strand, within RR42_RS24805 at 903.715 kb on - strand, within RR42_RS24805 at 903.715 kb on - strand, within RR42_RS24805 at 903.943 kb on - strand, within RR42_RS24805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 901,594 + RR42_RS24795 0.29 +0.2 901,595 - RR42_RS24795 0.29 -0.1 902,018 - RR42_RS24795 0.66 -0.2 902,323 + -1.1 902,324 - +1.3 902,434 + -0.6 902,435 - -0.4 902,562 - RR42_RS24800 0.23 +0.0 902,562 - RR42_RS24800 0.23 +0.6 902,609 + RR42_RS24800 0.33 -0.4 902,660 + RR42_RS24800 0.42 -0.0 902,661 - RR42_RS24800 0.43 +0.6 902,711 + RR42_RS24800 0.52 -0.0 902,712 - RR42_RS24800 0.53 +0.7 902,712 - RR42_RS24800 0.53 +0.4 902,747 + RR42_RS24800 0.59 -0.1 902,747 + RR42_RS24800 0.59 +0.7 902,773 - RR42_RS24800 0.64 +1.2 903,013 - -1.4 903,013 - -0.5 903,405 + RR42_RS24805 0.36 +0.6 903,439 - RR42_RS24805 0.39 -0.5 903,632 - RR42_RS24805 0.55 +1.2 903,679 - RR42_RS24805 0.59 -1.9 903,714 + RR42_RS24805 0.61 -1.1 903,715 - RR42_RS24805 0.62 -0.2 903,715 - RR42_RS24805 0.62 -0.0 903,943 - RR42_RS24805 0.80 -0.9
Or see this region's nucleotide sequence