Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23070 and RR42_RS23075 are separated by 31 nucleotides RR42_RS23075 and RR42_RS23080 are separated by 204 nucleotides
RR42_RS23070: RR42_RS23070 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 510,423 to 511,454
_RS23070
RR42_RS23075: RR42_RS23075 - ABC transporter substrate-binding protein, at 511,486 to 512,451
_RS23075
RR42_RS23080: RR42_RS23080 - LysR family transcriptional regulator, at 512,656 to 513,591
_RS23080
Position (kb)
511
512
513 Strain fitness (log2 ratio)
-2
-1
0
1 at 510.634 kb on + strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 510.635 kb on - strand, within RR42_RS23070 at 511.040 kb on + strand, within RR42_RS23070 at 511.154 kb on - strand, within RR42_RS23070 at 511.573 kb on - strand at 511.694 kb on + strand, within RR42_RS23075 at 511.695 kb on - strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.822 kb on - strand, within RR42_RS23075 at 511.907 kb on + strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.993 kb on - strand, within RR42_RS23075 at 512.221 kb on - strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.385 kb on - strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.470 kb on + strand at 512.526 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.537 kb on + strand at 512.537 kb on + strand at 512.836 kb on + strand, within RR42_RS23080 at 512.837 kb on - strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.084 kb on - strand, within RR42_RS23080 at 513.090 kb on - strand, within RR42_RS23080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 510,634 + RR42_RS23070 0.20 -1.3 510,635 - RR42_RS23070 0.21 +0.4 510,635 - RR42_RS23070 0.21 -1.2 510,635 - RR42_RS23070 0.21 -0.7 510,635 - RR42_RS23070 0.21 -2.1 511,040 + RR42_RS23070 0.60 +0.1 511,154 - RR42_RS23070 0.71 -0.7 511,573 - +0.3 511,694 + RR42_RS23075 0.22 +1.6 511,695 - RR42_RS23075 0.22 +0.4 511,821 + RR42_RS23075 0.35 -0.7 511,821 + RR42_RS23075 0.35 +0.5 511,822 - RR42_RS23075 0.35 +0.8 511,907 + RR42_RS23075 0.44 +0.2 511,908 - RR42_RS23075 0.44 -0.2 511,908 - RR42_RS23075 0.44 +1.4 511,992 + RR42_RS23075 0.52 -0.1 511,992 + RR42_RS23075 0.52 -0.1 511,992 + RR42_RS23075 0.52 -0.6 511,992 + RR42_RS23075 0.52 -0.4 511,993 - RR42_RS23075 0.52 -0.3 512,221 - RR42_RS23075 0.76 -0.0 512,235 + RR42_RS23075 0.78 -0.6 512,235 + RR42_RS23075 0.78 -0.9 512,236 - RR42_RS23075 0.78 +0.3 512,236 - RR42_RS23075 0.78 -0.4 512,236 - RR42_RS23075 0.78 +0.3 512,385 - +0.1 512,452 + +0.2 512,452 + -0.0 512,452 + +1.7 512,470 + -0.2 512,526 + +1.4 512,535 + +0.1 512,535 + -0.6 512,535 + -0.3 512,535 + +0.3 512,536 - -0.3 512,536 - -0.1 512,536 - -2.1 512,536 - -0.7 512,536 - -0.8 512,537 + +1.1 512,537 + +0.5 512,836 + RR42_RS23080 0.19 -1.2 512,837 - RR42_RS23080 0.19 +0.8 513,083 + RR42_RS23080 0.46 -0.9 513,083 + RR42_RS23080 0.46 -0.0 513,084 - RR42_RS23080 0.46 +0.2 513,090 - RR42_RS23080 0.46 -0.0
Or see this region's nucleotide sequence