Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22950 and RR42_RS22955 are separated by 126 nucleotides RR42_RS22955 and RR42_RS22960 are separated by 168 nucleotides
RR42_RS22950: RR42_RS22950 - cytochrome C550, at 482,692 to 483,549
_RS22950
RR42_RS22955: RR42_RS22955 - isocitrate dehydrogenase, at 483,676 to 484,707
_RS22955
RR42_RS22960: RR42_RS22960 - alpha/beta hydrolase, at 484,876 to 485,757
_RS22960
Position (kb) 483
484
485 Strain fitness (log2 ratio) -2
-1
0
1
2 at 482.679 kb on + strand at 482.680 kb on - strand at 482.710 kb on + strand at 482.772 kb on + strand at 482.773 kb on - strand at 482.773 kb on - strand at 482.791 kb on + strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.963 kb on - strand, within RR42_RS22950 at 482.963 kb on - strand, within RR42_RS22950 at 483.352 kb on - strand, within RR42_RS22950 at 483.352 kb on - strand, within RR42_RS22950 at 483.977 kb on + strand, within RR42_RS22955 at 483.977 kb on + strand, within RR42_RS22955 at 483.977 kb on + strand, within RR42_RS22955 at 484.017 kb on + strand, within RR42_RS22955 at 484.057 kb on + strand, within RR42_RS22955 at 484.058 kb on - strand, within RR42_RS22955 at 484.073 kb on - strand, within RR42_RS22955 at 484.073 kb on - strand, within RR42_RS22955 at 484.075 kb on + strand, within RR42_RS22955 at 484.076 kb on - strand, within RR42_RS22955 at 484.128 kb on + strand, within RR42_RS22955 at 484.171 kb on + strand, within RR42_RS22955 at 484.171 kb on + strand, within RR42_RS22955 at 484.280 kb on - strand, within RR42_RS22955 at 484.371 kb on + strand, within RR42_RS22955 at 484.895 kb on - strand at 484.895 kb on - strand at 485.054 kb on - strand, within RR42_RS22960 at 485.062 kb on + strand, within RR42_RS22960 at 485.062 kb on + strand, within RR42_RS22960 at 485.251 kb on + strand, within RR42_RS22960 at 485.252 kb on - strand, within RR42_RS22960 at 485.252 kb on - strand, within RR42_RS22960 at 485.285 kb on + strand, within RR42_RS22960 at 485.286 kb on - strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.517 kb on + strand, within RR42_RS22960 at 485.518 kb on - strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 482,679 + -0.1 482,680 - -0.3 482,710 + +1.6 482,772 + -0.2 482,773 - -0.9 482,773 - -1.8 482,791 + RR42_RS22950 0.12 -0.4 482,792 - RR42_RS22950 0.12 -0.6 482,792 - RR42_RS22950 0.12 +0.2 482,792 - RR42_RS22950 0.12 +0.4 482,809 + RR42_RS22950 0.14 -0.2 482,809 + RR42_RS22950 0.14 +0.8 482,809 + RR42_RS22950 0.14 +1.4 482,962 + RR42_RS22950 0.31 -0.2 482,962 + RR42_RS22950 0.31 -0.5 482,962 + RR42_RS22950 0.31 -0.3 482,962 + RR42_RS22950 0.31 -2.0 482,963 - RR42_RS22950 0.32 -1.4 482,963 - RR42_RS22950 0.32 -1.6 483,352 - RR42_RS22950 0.77 +0.5 483,352 - RR42_RS22950 0.77 -0.7 483,977 + RR42_RS22955 0.29 -0.7 483,977 + RR42_RS22955 0.29 +0.2 483,977 + RR42_RS22955 0.29 -0.8 484,017 + RR42_RS22955 0.33 -0.6 484,057 + RR42_RS22955 0.37 +0.5 484,058 - RR42_RS22955 0.37 -2.5 484,073 - RR42_RS22955 0.38 -0.2 484,073 - RR42_RS22955 0.38 -0.1 484,075 + RR42_RS22955 0.39 -0.3 484,076 - RR42_RS22955 0.39 +1.3 484,128 + RR42_RS22955 0.44 -1.4 484,171 + RR42_RS22955 0.48 -0.5 484,171 + RR42_RS22955 0.48 -0.8 484,280 - RR42_RS22955 0.59 -1.0 484,371 + RR42_RS22955 0.67 +0.4 484,895 - -1.6 484,895 - +0.1 485,054 - RR42_RS22960 0.20 +0.4 485,062 + RR42_RS22960 0.21 +2.0 485,062 + RR42_RS22960 0.21 -0.4 485,251 + RR42_RS22960 0.43 -0.7 485,252 - RR42_RS22960 0.43 -0.3 485,252 - RR42_RS22960 0.43 -0.2 485,285 + RR42_RS22960 0.46 -0.2 485,286 - RR42_RS22960 0.46 +0.4 485,428 + RR42_RS22960 0.63 -0.3 485,428 + RR42_RS22960 0.63 -0.7 485,428 + RR42_RS22960 0.63 -0.5 485,428 + RR42_RS22960 0.63 -0.2 485,428 + RR42_RS22960 0.63 +0.4 485,429 - RR42_RS22960 0.63 -0.4 485,429 - RR42_RS22960 0.63 -0.8 485,429 - RR42_RS22960 0.63 -0.4 485,429 - RR42_RS22960 0.63 +0.2 485,429 - RR42_RS22960 0.63 -0.1 485,517 + RR42_RS22960 0.73 -0.7 485,518 - RR42_RS22960 0.73 -0.3 485,551 + RR42_RS22960 0.77 -0.5 485,551 + RR42_RS22960 0.77 -0.3 485,551 + RR42_RS22960 0.77 -1.0 485,551 + RR42_RS22960 0.77 -1.9 485,552 - RR42_RS22960 0.77 +0.3 485,552 - RR42_RS22960 0.77 -1.2 485,552 - RR42_RS22960 0.77 -0.1
Or see this region's nucleotide sequence