Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22645 and RR42_RS22650 are separated by 230 nucleotides RR42_RS22650 and RR42_RS22655 are separated by 75 nucleotides
RR42_RS22645: RR42_RS22645 - GntR family transcriptional regulator, at 414,308 to 415,360
_RS22645
RR42_RS22650: RR42_RS22650 - ABC transporter substrate-binding protein, at 415,591 to 416,580
_RS22650
RR42_RS22655: RR42_RS22655 - mandelate racemase, at 416,656 to 417,813
_RS22655
Position (kb) 415
416
417 Strain fitness (log2 ratio) -2
-1
0
1
2 at 414.782 kb on - strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.782 kb on - strand, within RR42_RS22645 at 414.869 kb on + strand, within RR42_RS22645 at 415.388 kb on + strand at 415.527 kb on + strand at 415.527 kb on + strand at 415.687 kb on + strand at 415.688 kb on - strand at 415.870 kb on + strand, within RR42_RS22650 at 415.871 kb on - strand, within RR42_RS22650 at 415.912 kb on + strand, within RR42_RS22650 at 415.913 kb on - strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.159 kb on - strand, within RR42_RS22650 at 416.277 kb on - strand, within RR42_RS22650 at 416.302 kb on + strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.531 kb on + strand at 416.579 kb on - strand at 416.725 kb on + strand at 416.854 kb on + strand, within RR42_RS22655 at 416.854 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.110 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.460 kb on - strand, within RR42_RS22655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 414,782 - RR42_RS22645 0.45 +0.2 414,782 - RR42_RS22645 0.45 +2.0 414,782 - RR42_RS22645 0.45 -0.0 414,869 + RR42_RS22645 0.53 +1.1 415,388 + +0.4 415,527 + -0.0 415,527 + -0.6 415,687 + +0.5 415,688 - -0.3 415,870 + RR42_RS22650 0.28 +0.5 415,871 - RR42_RS22650 0.28 -0.3 415,912 + RR42_RS22650 0.32 +0.5 415,913 - RR42_RS22650 0.33 +1.2 415,921 + RR42_RS22650 0.33 -0.3 415,921 + RR42_RS22650 0.33 +0.8 415,922 - RR42_RS22650 0.33 +0.0 415,922 - RR42_RS22650 0.33 -0.2 415,922 - RR42_RS22650 0.33 -0.3 415,922 - RR42_RS22650 0.33 -0.2 416,065 + RR42_RS22650 0.48 -2.5 416,065 + RR42_RS22650 0.48 +0.7 416,158 + RR42_RS22650 0.57 +0.8 416,158 + RR42_RS22650 0.57 -0.7 416,159 - RR42_RS22650 0.57 -1.6 416,277 - RR42_RS22650 0.69 +1.0 416,302 + RR42_RS22650 0.72 +0.3 416,386 - RR42_RS22650 0.80 -0.4 416,386 - RR42_RS22650 0.80 +1.5 416,531 + +0.3 416,579 - -1.5 416,725 + +0.4 416,854 + RR42_RS22655 0.17 -0.9 416,854 + RR42_RS22655 0.17 -0.3 417,109 + +1.0 417,109 + RR42_RS22655 0.39 -0.3 417,109 + RR42_RS22655 0.39 +0.2 417,109 + RR42_RS22655 0.39 +0.4 417,109 + RR42_RS22655 0.39 +0.3 417,110 - RR42_RS22655 0.39 +0.3 417,136 - RR42_RS22655 0.41 +0.6 417,136 - RR42_RS22655 0.41 -0.0 417,460 - RR42_RS22655 0.69 +1.1
Or see this region's nucleotide sequence