Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22425 and RR42_RS22430 are separated by 8 nucleotides RR42_RS22430 and RR42_RS22435 overlap by 4 nucleotides RR42_RS22435 and RR42_RS22440 are separated by 32 nucleotides RR42_RS22440 and RR42_RS22445 overlap by 4 nucleotides
RR42_RS22425: RR42_RS22425 - CDP-alcohol phosphatidyltransferase, at 367,761 to 368,429
_RS22425
RR42_RS22430: RR42_RS22430 - 2OG-Fe(II) oxygenase, at 368,438 to 369,280
_RS22430
RR42_RS22435: RR42_RS22435 - membrane protein, at 369,277 to 370,284
_RS22435
RR42_RS22440: RR42_RS22440 - ADP-glucose pyrophosphorylase, at 370,317 to 371,081
_RS22440
RR42_RS22445: RR42_RS22445 - phosphoenolpyruvate phosphomutase, at 371,078 to 372,784
_RS22445
Position (kb)
369
370
371 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 368.277 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.377 kb on - strand at 368.714 kb on + strand, within RR42_RS22430 at 368.715 kb on - strand, within RR42_RS22430 at 368.769 kb on + strand, within RR42_RS22430 at 368.793 kb on - strand, within RR42_RS22430 at 368.793 kb on - strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.877 kb on - strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.886 kb on - strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.889 kb on - strand, within RR42_RS22430 at 368.889 kb on - strand, within RR42_RS22430 at 368.891 kb on + strand, within RR42_RS22430 at 368.891 kb on + strand, within RR42_RS22430 at 368.892 kb on - strand, within RR42_RS22430 at 368.936 kb on + strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.940 kb on - strand, within RR42_RS22430 at 369.002 kb on + strand, within RR42_RS22430 at 369.155 kb on + strand, within RR42_RS22430 at 369.156 kb on - strand, within RR42_RS22430 at 369.156 kb on - strand, within RR42_RS22430 at 369.202 kb on + strand at 369.221 kb on + strand at 369.448 kb on + strand, within RR42_RS22435 at 369.448 kb on + strand, within RR42_RS22435 at 369.449 kb on - strand, within RR42_RS22435 at 369.449 kb on - strand, within RR42_RS22435 at 369.449 kb on - strand, within RR42_RS22435 at 369.449 kb on - strand, within RR42_RS22435 at 369.449 kb on - strand, within RR42_RS22435 at 369.764 kb on + strand, within RR42_RS22435 at 369.765 kb on - strand, within RR42_RS22435 at 369.765 kb on - strand, within RR42_RS22435 at 369.765 kb on - strand, within RR42_RS22435 at 369.765 kb on - strand, within RR42_RS22435 at 369.770 kb on + strand, within RR42_RS22435 at 369.770 kb on + strand, within RR42_RS22435 at 369.770 kb on + strand, within RR42_RS22435 at 369.771 kb on - strand, within RR42_RS22435 at 369.781 kb on + strand, within RR42_RS22435 at 369.782 kb on - strand, within RR42_RS22435 at 369.836 kb on + strand, within RR42_RS22435 at 369.907 kb on + strand, within RR42_RS22435 at 369.907 kb on + strand, within RR42_RS22435 at 369.907 kb on + strand, within RR42_RS22435 at 369.908 kb on - strand, within RR42_RS22435 at 369.908 kb on - strand, within RR42_RS22435 at 370.216 kb on + strand at 370.216 kb on + strand at 370.220 kb on + strand at 370.346 kb on - strand at 370.650 kb on + strand, within RR42_RS22440 at 370.651 kb on - strand, within RR42_RS22440 at 370.651 kb on - strand, within RR42_RS22440 at 370.806 kb on + strand, within RR42_RS22440 at 370.807 kb on - strand, within RR42_RS22440 at 370.887 kb on + strand, within RR42_RS22440 at 370.887 kb on + strand, within RR42_RS22440 at 370.888 kb on - strand, within RR42_RS22440 at 370.960 kb on + strand, within RR42_RS22440 at 370.960 kb on + strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440 at 370.961 kb on - strand, within RR42_RS22440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 368,277 + RR42_RS22425 0.77 -0.9 368,277 + RR42_RS22425 0.77 -0.1 368,278 - RR42_RS22425 0.77 +0.2 368,278 - RR42_RS22425 0.77 -0.3 368,278 - RR42_RS22425 0.77 -0.6 368,377 - -0.4 368,714 + RR42_RS22430 0.33 -0.4 368,715 - RR42_RS22430 0.33 -1.3 368,769 + RR42_RS22430 0.39 -0.4 368,793 - RR42_RS22430 0.42 +0.3 368,793 - RR42_RS22430 0.42 -2.0 368,876 + RR42_RS22430 0.52 -0.2 368,876 + RR42_RS22430 0.52 +0.3 368,876 + RR42_RS22430 0.52 +0.2 368,877 - RR42_RS22430 0.52 +0.5 368,885 + RR42_RS22430 0.53 +0.0 368,885 + RR42_RS22430 0.53 -0.2 368,885 + RR42_RS22430 0.53 +0.1 368,885 + RR42_RS22430 0.53 +0.5 368,885 + RR42_RS22430 0.53 -0.5 368,885 + RR42_RS22430 0.53 +0.1 368,886 - RR42_RS22430 0.53 +0.2 368,888 + RR42_RS22430 0.53 -0.5 368,888 + RR42_RS22430 0.53 -0.2 368,888 + RR42_RS22430 0.53 +0.6 368,888 + RR42_RS22430 0.53 -1.0 368,889 - RR42_RS22430 0.53 +2.0 368,889 - RR42_RS22430 0.53 +0.8 368,891 + RR42_RS22430 0.54 -1.4 368,891 + RR42_RS22430 0.54 +0.1 368,892 - RR42_RS22430 0.54 -0.7 368,936 + RR42_RS22430 0.59 -0.3 368,937 - RR42_RS22430 0.59 -0.2 368,937 - RR42_RS22430 0.59 -0.1 368,937 - RR42_RS22430 0.59 +0.1 368,937 - RR42_RS22430 0.59 +0.0 368,940 - RR42_RS22430 0.60 +0.8 369,002 + RR42_RS22430 0.67 -0.6 369,155 + RR42_RS22430 0.85 -0.4 369,156 - RR42_RS22430 0.85 -0.2 369,156 - RR42_RS22430 0.85 +0.1 369,202 + -0.1 369,221 + -0.4 369,448 + RR42_RS22435 0.17 -0.8 369,448 + RR42_RS22435 0.17 -2.1 369,449 - RR42_RS22435 0.17 -0.4 369,449 - RR42_RS22435 0.17 +1.0 369,449 - RR42_RS22435 0.17 +0.2 369,449 - RR42_RS22435 0.17 -0.3 369,449 - RR42_RS22435 0.17 -0.8 369,764 + RR42_RS22435 0.48 -2.2 369,765 - RR42_RS22435 0.48 -0.4 369,765 - RR42_RS22435 0.48 +0.4 369,765 - RR42_RS22435 0.48 -0.7 369,765 - RR42_RS22435 0.48 -1.6 369,770 + RR42_RS22435 0.49 -0.6 369,770 + RR42_RS22435 0.49 -1.5 369,770 + RR42_RS22435 0.49 -1.3 369,771 - RR42_RS22435 0.49 -1.6 369,781 + RR42_RS22435 0.50 -1.6 369,782 - RR42_RS22435 0.50 -0.3 369,836 + RR42_RS22435 0.55 -0.7 369,907 + RR42_RS22435 0.62 -1.1 369,907 + RR42_RS22435 0.62 -2.4 369,907 + RR42_RS22435 0.62 -3.0 369,908 - RR42_RS22435 0.63 -0.2 369,908 - RR42_RS22435 0.63 -1.4 370,216 + -0.9 370,216 + -1.3 370,220 + -0.4 370,346 - -0.6 370,650 + RR42_RS22440 0.44 +1.3 370,651 - RR42_RS22440 0.44 +1.3 370,651 - RR42_RS22440 0.44 -0.6 370,806 + RR42_RS22440 0.64 -0.4 370,807 - RR42_RS22440 0.64 +2.6 370,887 + RR42_RS22440 0.75 -0.5 370,887 + RR42_RS22440 0.75 -0.3 370,888 - RR42_RS22440 0.75 -1.1 370,960 + RR42_RS22440 0.84 -0.8 370,960 + RR42_RS22440 0.84 -0.3 370,961 - RR42_RS22440 0.84 +0.3 370,961 - RR42_RS22440 0.84 +1.0 370,961 - RR42_RS22440 0.84 +0.3 370,961 - RR42_RS22440 0.84 -0.5 370,961 - RR42_RS22440 0.84 +0.1
Or see this region's nucleotide sequence