Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22420 and RR42_RS22425 are separated by 312 nucleotides RR42_RS22425 and RR42_RS22430 are separated by 8 nucleotides RR42_RS22430 and RR42_RS22435 overlap by 4 nucleotides
RR42_RS22420: RR42_RS22420 - penicillin-binding protein, at 365,025 to 367,448
_RS22420
RR42_RS22425: RR42_RS22425 - CDP-alcohol phosphatidyltransferase, at 367,761 to 368,429
_RS22425
RR42_RS22430: RR42_RS22430 - 2OG-Fe(II) oxygenase, at 368,438 to 369,280
_RS22430
RR42_RS22435: RR42_RS22435 - membrane protein, at 369,277 to 370,284
_RS22435
Position (kb) 367
368
369 Strain fitness (log2 ratio) -2
-1
0
1
2 at 366.997 kb on - strand, within RR42_RS22420 at 367.138 kb on + strand, within RR42_RS22420 at 367.618 kb on + strand at 367.618 kb on + strand at 367.619 kb on - strand at 367.619 kb on - strand at 367.640 kb on + strand at 367.712 kb on + strand at 367.713 kb on - strand at 367.715 kb on + strand at 367.715 kb on + strand at 367.716 kb on - strand at 367.716 kb on - strand at 367.809 kb on - strand at 367.809 kb on - strand at 367.953 kb on + strand, within RR42_RS22425 at 367.953 kb on + strand, within RR42_RS22425 at 368.038 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.277 kb on + strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.278 kb on - strand, within RR42_RS22425 at 368.377 kb on - strand at 368.714 kb on + strand, within RR42_RS22430 at 368.715 kb on - strand, within RR42_RS22430 at 368.769 kb on + strand, within RR42_RS22430 at 368.793 kb on - strand, within RR42_RS22430 at 368.793 kb on - strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.876 kb on + strand, within RR42_RS22430 at 368.877 kb on - strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.885 kb on + strand, within RR42_RS22430 at 368.886 kb on - strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.888 kb on + strand, within RR42_RS22430 at 368.889 kb on - strand, within RR42_RS22430 at 368.889 kb on - strand, within RR42_RS22430 at 368.891 kb on + strand, within RR42_RS22430 at 368.891 kb on + strand, within RR42_RS22430 at 368.892 kb on - strand, within RR42_RS22430 at 368.936 kb on + strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.937 kb on - strand, within RR42_RS22430 at 368.940 kb on - strand, within RR42_RS22430 at 369.002 kb on + strand, within RR42_RS22430 at 369.155 kb on + strand, within RR42_RS22430 at 369.156 kb on - strand, within RR42_RS22430 at 369.156 kb on - strand, within RR42_RS22430 at 369.202 kb on + strand at 369.221 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 366,997 - RR42_RS22420 0.81 -0.7 367,138 + RR42_RS22420 0.87 -0.9 367,618 + -1.0 367,618 + +0.8 367,619 - +0.6 367,619 - +0.4 367,640 + +0.5 367,712 + -0.1 367,713 - +0.1 367,715 + +0.4 367,715 + -0.8 367,716 - -0.5 367,716 - +0.0 367,809 - +1.0 367,809 - +1.1 367,953 + RR42_RS22425 0.29 -2.6 367,953 + RR42_RS22425 0.29 +0.1 368,038 + RR42_RS22425 0.41 -0.7 368,277 + RR42_RS22425 0.77 -0.9 368,277 + RR42_RS22425 0.77 -0.1 368,278 - RR42_RS22425 0.77 +0.2 368,278 - RR42_RS22425 0.77 -0.3 368,278 - RR42_RS22425 0.77 -0.6 368,377 - -0.4 368,714 + RR42_RS22430 0.33 -0.4 368,715 - RR42_RS22430 0.33 -1.3 368,769 + RR42_RS22430 0.39 -0.4 368,793 - RR42_RS22430 0.42 +0.3 368,793 - RR42_RS22430 0.42 -2.0 368,876 + RR42_RS22430 0.52 -0.2 368,876 + RR42_RS22430 0.52 +0.3 368,876 + RR42_RS22430 0.52 +0.2 368,877 - RR42_RS22430 0.52 +0.5 368,885 + RR42_RS22430 0.53 +0.0 368,885 + RR42_RS22430 0.53 -0.2 368,885 + RR42_RS22430 0.53 +0.1 368,885 + RR42_RS22430 0.53 +0.5 368,885 + RR42_RS22430 0.53 -0.5 368,885 + RR42_RS22430 0.53 +0.1 368,886 - RR42_RS22430 0.53 +0.2 368,888 + RR42_RS22430 0.53 -0.5 368,888 + RR42_RS22430 0.53 -0.2 368,888 + RR42_RS22430 0.53 +0.6 368,888 + RR42_RS22430 0.53 -1.0 368,889 - RR42_RS22430 0.53 +2.0 368,889 - RR42_RS22430 0.53 +0.8 368,891 + RR42_RS22430 0.54 -1.4 368,891 + RR42_RS22430 0.54 +0.1 368,892 - RR42_RS22430 0.54 -0.7 368,936 + RR42_RS22430 0.59 -0.3 368,937 - RR42_RS22430 0.59 -0.2 368,937 - RR42_RS22430 0.59 -0.1 368,937 - RR42_RS22430 0.59 +0.1 368,937 - RR42_RS22430 0.59 +0.0 368,940 - RR42_RS22430 0.60 +0.8 369,002 + RR42_RS22430 0.67 -0.6 369,155 + RR42_RS22430 0.85 -0.4 369,156 - RR42_RS22430 0.85 -0.2 369,156 - RR42_RS22430 0.85 +0.1 369,202 + -0.1 369,221 + -0.4
Or see this region's nucleotide sequence