Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21515 and RR42_RS21520 are separated by 226 nucleotides RR42_RS21520 and RR42_RS21525 are separated by 23 nucleotides RR42_RS21525 and RR42_RS21530 are separated by 47 nucleotides
RR42_RS21515: RR42_RS21515 - LysR family transcriptional regulator, at 154,775 to 155,713
_RS21515
RR42_RS21520: RR42_RS21520 - flagellar brake protein, at 155,940 to 156,689
_RS21520
RR42_RS21525: RR42_RS21525 - hypothetical protein, at 156,713 to 157,810
_RS21525
RR42_RS21530: RR42_RS21530 - IclR family transcriptional regulator, at 157,858 to 158,706
_RS21530
Position (kb) 156
157
158 Strain fitness (log2 ratio) -2
-1
0
1
2 at 155.819 kb on + strand at 155.819 kb on + strand at 155.820 kb on - strand at 155.820 kb on - strand at 155.820 kb on - strand at 155.820 kb on - strand at 156.270 kb on + strand, within RR42_RS21520 at 156.270 kb on + strand, within RR42_RS21520 at 156.271 kb on - strand, within RR42_RS21520 at 156.369 kb on + strand, within RR42_RS21520 at 156.369 kb on + strand, within RR42_RS21520 at 156.369 kb on + strand, within RR42_RS21520 at 156.370 kb on - strand, within RR42_RS21520 at 156.370 kb on - strand, within RR42_RS21520 at 156.370 kb on - strand, within RR42_RS21520 at 156.370 kb on - strand, within RR42_RS21520 at 156.370 kb on - strand, within RR42_RS21520 at 156.370 kb on - strand, within RR42_RS21520 at 156.416 kb on + strand, within RR42_RS21520 at 156.416 kb on + strand, within RR42_RS21520 at 156.763 kb on - strand at 156.763 kb on - strand at 157.046 kb on + strand, within RR42_RS21525 at 157.296 kb on + strand, within RR42_RS21525 at 157.297 kb on - strand, within RR42_RS21525 at 157.297 kb on - strand at 157.402 kb on + strand, within RR42_RS21525 at 157.402 kb on + strand, within RR42_RS21525 at 157.403 kb on - strand, within RR42_RS21525 at 157.403 kb on - strand, within RR42_RS21525 at 157.469 kb on + strand, within RR42_RS21525 at 157.470 kb on - strand, within RR42_RS21525 at 157.470 kb on - strand, within RR42_RS21525 at 157.750 kb on - strand at 157.750 kb on - strand at 157.793 kb on - strand at 157.939 kb on + strand at 157.940 kb on - strand at 158.054 kb on + strand, within RR42_RS21530 at 158.054 kb on + strand, within RR42_RS21530 at 158.054 kb on + strand, within RR42_RS21530 at 158.054 kb on + strand, within RR42_RS21530 at 158.054 kb on + strand, within RR42_RS21530 at 158.055 kb on - strand, within RR42_RS21530 at 158.055 kb on - strand, within RR42_RS21530 at 158.055 kb on - strand, within RR42_RS21530 at 158.055 kb on - strand, within RR42_RS21530 at 158.055 kb on - strand, within RR42_RS21530 at 158.210 kb on + strand, within RR42_RS21530 at 158.210 kb on + strand, within RR42_RS21530 at 158.280 kb on - strand, within RR42_RS21530 at 158.390 kb on + strand, within RR42_RS21530 at 158.390 kb on + strand, within RR42_RS21530 at 158.390 kb on + strand, within RR42_RS21530 at 158.390 kb on + strand, within RR42_RS21530 at 158.390 kb on + strand, within RR42_RS21530 at 158.390 kb on + strand, within RR42_RS21530 at 158.391 kb on - strand, within RR42_RS21530 at 158.391 kb on - strand, within RR42_RS21530 at 158.391 kb on - strand, within RR42_RS21530 at 158.475 kb on - strand, within RR42_RS21530 at 158.547 kb on + strand, within RR42_RS21530 at 158.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 155,819 + +1.3 155,819 + -0.2 155,820 - +0.1 155,820 - +1.0 155,820 - -0.7 155,820 - -0.3 156,270 + RR42_RS21520 0.44 +0.8 156,270 + RR42_RS21520 0.44 +1.3 156,271 - RR42_RS21520 0.44 -0.0 156,369 + RR42_RS21520 0.57 +1.0 156,369 + RR42_RS21520 0.57 -0.6 156,369 + RR42_RS21520 0.57 -1.1 156,370 - RR42_RS21520 0.57 +0.8 156,370 - RR42_RS21520 0.57 -0.2 156,370 - RR42_RS21520 0.57 +1.4 156,370 - RR42_RS21520 0.57 -0.3 156,370 - RR42_RS21520 0.57 +0.2 156,370 - RR42_RS21520 0.57 -0.3 156,416 + RR42_RS21520 0.63 -0.4 156,416 + RR42_RS21520 0.63 -0.7 156,763 - -0.7 156,763 - +0.2 157,046 + RR42_RS21525 0.30 -0.1 157,296 + RR42_RS21525 0.53 -1.9 157,297 - RR42_RS21525 0.53 -0.8 157,297 - +2.0 157,402 + RR42_RS21525 0.63 +0.4 157,402 + RR42_RS21525 0.63 -0.4 157,403 - RR42_RS21525 0.63 -0.7 157,403 - RR42_RS21525 0.63 -0.7 157,469 + RR42_RS21525 0.69 -0.6 157,470 - RR42_RS21525 0.69 -0.0 157,470 - RR42_RS21525 0.69 +0.6 157,750 - -1.3 157,750 - -0.1 157,793 - +0.9 157,939 + +0.2 157,940 - -0.1 158,054 + RR42_RS21530 0.23 +1.8 158,054 + RR42_RS21530 0.23 -1.6 158,054 + RR42_RS21530 0.23 -1.5 158,054 + RR42_RS21530 0.23 +0.2 158,054 + RR42_RS21530 0.23 -0.1 158,055 - RR42_RS21530 0.23 +0.8 158,055 - RR42_RS21530 0.23 +0.1 158,055 - RR42_RS21530 0.23 -0.9 158,055 - RR42_RS21530 0.23 +0.2 158,055 - RR42_RS21530 0.23 +0.4 158,210 + RR42_RS21530 0.41 -0.1 158,210 + RR42_RS21530 0.41 +0.1 158,280 - RR42_RS21530 0.50 -1.2 158,390 + RR42_RS21530 0.63 -0.3 158,390 + RR42_RS21530 0.63 -0.1 158,390 + RR42_RS21530 0.63 -2.4 158,390 + RR42_RS21530 0.63 +0.3 158,390 + RR42_RS21530 0.63 +1.0 158,390 + RR42_RS21530 0.63 -0.5 158,391 - RR42_RS21530 0.63 +0.6 158,391 - RR42_RS21530 0.63 +1.1 158,391 - RR42_RS21530 0.63 +0.0 158,475 - RR42_RS21530 0.73 +0.7 158,547 + RR42_RS21530 0.81 +0.1 158,748 - +1.3
Or see this region's nucleotide sequence