Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19240 and RR42_RS19245 are separated by 2 nucleotides RR42_RS19245 and RR42_RS19250 overlap by 4 nucleotides RR42_RS19250 and RR42_RS19255 are separated by 11 nucleotides RR42_RS19255 and RR42_RS19260 are separated by 236 nucleotides RR42_RS19260 and RR42_RS19265 are separated by 1 nucleotides
RR42_RS19240: RR42_RS19240 - 50S ribosomal protein L2, at 4,155,745 to 4,156,575
_RS19240
RR42_RS19245: RR42_RS19245 - 50S ribosomal protein L23, at 4,156,578 to 4,156,892
_RS19245
RR42_RS19250: RR42_RS19250 - 50S ribosomal protein L4, at 4,156,889 to 4,157,509
_RS19250
RR42_RS19255: RR42_RS19255 - 50S ribosomal protein L3, at 4,157,521 to 4,158,171
_RS19255
RR42_RS19260: RR42_RS19260 - nitrilotriacetate monooxygenase, at 4,158,408 to 4,158,899
_RS19260
RR42_RS19265: RR42_RS19265 - alanine-phosphoribitol ligase, at 4,158,901 to 4,160,142
_RS19265
Position (kb) 4157
4158
4159 Strain fitness (log2 ratio) -1
0
1
2 at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.358 kb on + strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.359 kb on - strand at 4158.409 kb on + strand at 4158.565 kb on + strand, within RR42_RS19260 at 4158.565 kb on + strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4158.566 kb on - strand, within RR42_RS19260 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.118 kb on + strand, within RR42_RS19265 at 4159.119 kb on - strand, within RR42_RS19265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 4,158,358 + +0.2 4,158,358 + +0.3 4,158,358 + -0.3 4,158,359 - -1.4 4,158,359 - +1.1 4,158,359 - +1.0 4,158,359 - +2.0 4,158,409 + -0.5 4,158,565 + RR42_RS19260 0.32 +0.1 4,158,565 + RR42_RS19260 0.32 +0.4 4,158,566 - RR42_RS19260 0.32 +0.4 4,158,566 - RR42_RS19260 0.32 +0.7 4,159,118 + RR42_RS19265 0.17 +0.0 4,159,118 + RR42_RS19265 0.17 +0.8 4,159,119 - RR42_RS19265 0.18 -0.9
Or see this region's nucleotide sequence