Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15330 and RR42_RS15335 are separated by 16 nucleotides RR42_RS15335 and RR42_RS15340 are separated by 50 nucleotides RR42_RS15340 and RR42_RS15345 are separated by 92 nucleotides
RR42_RS15330: RR42_RS15330 - hypothetical protein, at 3,316,896 to 3,317,981
_RS15330
RR42_RS15335: RR42_RS15335 - peptide methionine sulfoxide reductase, at 3,317,998 to 3,318,540
_RS15335
RR42_RS15340: RR42_RS15340 - hypothetical protein, at 3,318,591 to 3,318,866
_RS15340
RR42_RS15345: RR42_RS15345 - long-chain fatty acid--CoA ligase, at 3,318,959 to 3,320,512
_RS15345
Position (kb)
3318
3319 Strain fitness (log2 ratio)
-2
-1
0
1 at 3317.618 kb on + strand, within RR42_RS15330 at 3317.618 kb on + strand, within RR42_RS15330 at 3317.619 kb on - strand, within RR42_RS15330 at 3317.772 kb on + strand, within RR42_RS15330 at 3317.793 kb on + strand, within RR42_RS15330 at 3317.889 kb on + strand at 3317.889 kb on + strand at 3317.889 kb on + strand at 3317.890 kb on - strand at 3318.011 kb on + strand at 3318.011 kb on + strand at 3318.012 kb on - strand at 3318.068 kb on + strand, within RR42_RS15335 at 3318.095 kb on + strand, within RR42_RS15335 at 3318.095 kb on + strand, within RR42_RS15335 at 3318.096 kb on - strand, within RR42_RS15335 at 3318.125 kb on + strand, within RR42_RS15335 at 3318.126 kb on - strand, within RR42_RS15335 at 3318.200 kb on + strand, within RR42_RS15335 at 3318.200 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.421 kb on + strand, within RR42_RS15335 at 3318.422 kb on - strand, within RR42_RS15335 at 3318.422 kb on - strand, within RR42_RS15335 at 3318.435 kb on + strand, within RR42_RS15335 at 3318.437 kb on + strand, within RR42_RS15335 at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.565 kb on + strand at 3318.566 kb on - strand at 3318.569 kb on + strand at 3318.569 kb on + strand at 3318.569 kb on + strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.570 kb on - strand at 3318.575 kb on - strand at 3318.575 kb on - strand at 3318.575 kb on - strand at 3318.702 kb on - strand, within RR42_RS15340 at 3318.716 kb on - strand, within RR42_RS15340 at 3318.716 kb on - strand, within RR42_RS15340 at 3318.788 kb on - strand, within RR42_RS15340 at 3318.788 kb on - strand, within RR42_RS15340 at 3318.791 kb on + strand, within RR42_RS15340 at 3318.792 kb on - strand, within RR42_RS15340 at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.868 kb on + strand at 3318.869 kb on - strand at 3318.869 kb on - strand at 3318.869 kb on - strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.870 kb on + strand at 3318.871 kb on - strand at 3318.871 kb on - strand at 3318.871 kb on - strand at 3318.878 kb on - strand at 3318.941 kb on + strand at 3318.941 kb on + strand at 3318.942 kb on - strand at 3319.252 kb on - strand, within RR42_RS15345 at 3319.389 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 3,317,618 + RR42_RS15330 0.66 +0.2 3,317,618 + RR42_RS15330 0.66 -0.0 3,317,619 - RR42_RS15330 0.67 -1.5 3,317,772 + RR42_RS15330 0.81 -0.5 3,317,793 + RR42_RS15330 0.83 +0.3 3,317,889 + -0.0 3,317,889 + +0.3 3,317,889 + +0.0 3,317,890 - +0.3 3,318,011 + -1.8 3,318,011 + +1.0 3,318,012 - +0.1 3,318,068 + RR42_RS15335 0.13 +0.0 3,318,095 + RR42_RS15335 0.18 -1.1 3,318,095 + RR42_RS15335 0.18 -0.1 3,318,096 - RR42_RS15335 0.18 +0.4 3,318,125 + RR42_RS15335 0.23 +0.4 3,318,126 - RR42_RS15335 0.24 +0.1 3,318,200 + RR42_RS15335 0.37 +0.1 3,318,200 + RR42_RS15335 0.37 +0.4 3,318,421 + RR42_RS15335 0.78 -0.0 3,318,421 + RR42_RS15335 0.78 -0.7 3,318,421 + RR42_RS15335 0.78 -0.7 3,318,422 - RR42_RS15335 0.78 +0.7 3,318,422 - RR42_RS15335 0.78 +0.8 3,318,435 + RR42_RS15335 0.80 -0.6 3,318,437 + RR42_RS15335 0.81 -0.1 3,318,565 + +0.1 3,318,565 + +0.4 3,318,565 + +0.4 3,318,565 + +0.9 3,318,566 - +0.9 3,318,569 + +1.1 3,318,569 + +0.7 3,318,569 + -1.7 3,318,570 - -2.5 3,318,570 - +1.0 3,318,570 - +0.4 3,318,570 - +0.9 3,318,575 - +0.0 3,318,575 - -0.0 3,318,575 - -0.1 3,318,702 - RR42_RS15340 0.40 -0.2 3,318,716 - RR42_RS15340 0.45 +0.2 3,318,716 - RR42_RS15340 0.45 +0.6 3,318,788 - RR42_RS15340 0.71 +1.3 3,318,788 - RR42_RS15340 0.71 +0.6 3,318,791 + RR42_RS15340 0.72 +0.9 3,318,792 - RR42_RS15340 0.73 +0.4 3,318,868 + +0.2 3,318,868 + -0.3 3,318,868 + -0.2 3,318,868 + +0.3 3,318,868 + -0.9 3,318,868 + -0.6 3,318,869 - -1.1 3,318,869 - -0.4 3,318,869 - +0.7 3,318,870 + +0.1 3,318,870 + +0.6 3,318,870 + +0.1 3,318,870 + +0.0 3,318,871 - +1.2 3,318,871 - +1.1 3,318,871 - +1.2 3,318,878 - +0.8 3,318,941 + +0.1 3,318,941 + -0.1 3,318,942 - +0.3 3,319,252 - RR42_RS15345 0.19 -0.6 3,319,389 + RR42_RS15345 0.28 -0.2 3,319,620 + RR42_RS15345 0.43 -0.5 3,319,620 + RR42_RS15345 0.43 +0.2 3,319,620 + RR42_RS15345 0.43 +0.5 3,319,620 + RR42_RS15345 0.43 +0.9 3,319,621 - RR42_RS15345 0.43 +0.5 3,319,621 - RR42_RS15345 0.43 -1.1 3,319,621 - RR42_RS15345 0.43 +0.4 3,319,621 - RR42_RS15345 0.43 +0.3 3,319,621 - RR42_RS15345 0.43 +0.0
Or see this region's nucleotide sequence