Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14635 and RR42_RS14640 are separated by 88 nucleotides RR42_RS14640 and RR42_RS14645 are separated by 2 nucleotides
RR42_RS14635: RR42_RS14635 - proline-specific permease, at 3,147,825 to 3,149,225
_RS14635
RR42_RS14640: RR42_RS14640 - histidine ammonia-lyase, at 3,149,314 to 3,150,846
_RS14640
RR42_RS14645: RR42_RS14645 - urocanate hydratase, at 3,150,849 to 3,152,534
_RS14645
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3148.355 kb on + strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.445 kb on - strand, within RR42_RS14635 at 3148.477 kb on + strand, within RR42_RS14635 at 3148.477 kb on + strand, within RR42_RS14635 at 3148.477 kb on + strand, within RR42_RS14635 at 3148.478 kb on - strand, within RR42_RS14635 at 3148.777 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.885 kb on + strand, within RR42_RS14635 at 3148.886 kb on - strand, within RR42_RS14635 at 3148.939 kb on + strand, within RR42_RS14635 at 3148.939 kb on + strand, within RR42_RS14635 at 3148.940 kb on - strand, within RR42_RS14635 at 3149.081 kb on - strand, within RR42_RS14635 at 3149.228 kb on - strand at 3149.420 kb on + strand at 3149.421 kb on - strand at 3149.423 kb on + strand at 3149.424 kb on - strand at 3149.754 kb on + strand, within RR42_RS14640 at 3149.755 kb on - strand, within RR42_RS14640 at 3150.058 kb on - strand, within RR42_RS14640 at 3150.077 kb on + strand, within RR42_RS14640 at 3150.077 kb on + strand, within RR42_RS14640 at 3150.078 kb on - strand, within RR42_RS14640 at 3150.078 kb on - strand, within RR42_RS14640 at 3150.184 kb on + strand, within RR42_RS14640 at 3150.247 kb on + strand, within RR42_RS14640 at 3150.248 kb on - strand, within RR42_RS14640 at 3150.390 kb on + strand, within RR42_RS14640 at 3150.528 kb on + strand, within RR42_RS14640 at 3150.529 kb on - strand, within RR42_RS14640 at 3150.788 kb on + strand at 3150.789 kb on - strand at 3151.414 kb on + strand at 3151.414 kb on + strand, within RR42_RS14645 at 3151.415 kb on - strand, within RR42_RS14645 at 3151.415 kb on - strand, within RR42_RS14645 at 3151.415 kb on - strand, within RR42_RS14645 at 3151.489 kb on + strand, within RR42_RS14645 at 3151.489 kb on + strand, within RR42_RS14645 at 3151.489 kb on + strand, within RR42_RS14645 at 3151.490 kb on - strand, within RR42_RS14645 at 3151.490 kb on - strand, within RR42_RS14645 at 3151.685 kb on - strand, within RR42_RS14645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 3,148,355 + RR42_RS14635 0.38 +0.8 3,148,445 - RR42_RS14635 0.44 -0.7 3,148,445 - RR42_RS14635 0.44 -2.0 3,148,445 - RR42_RS14635 0.44 +1.0 3,148,445 - RR42_RS14635 0.44 -0.8 3,148,477 + RR42_RS14635 0.47 -0.8 3,148,477 + RR42_RS14635 0.47 +1.2 3,148,477 + RR42_RS14635 0.47 -0.3 3,148,478 - RR42_RS14635 0.47 +0.5 3,148,777 + RR42_RS14635 0.68 +1.2 3,148,885 + RR42_RS14635 0.76 -1.5 3,148,885 + RR42_RS14635 0.76 +0.2 3,148,885 + RR42_RS14635 0.76 -0.2 3,148,885 + RR42_RS14635 0.76 +1.7 3,148,885 + RR42_RS14635 0.76 +1.0 3,148,886 - RR42_RS14635 0.76 +1.6 3,148,939 + RR42_RS14635 0.80 -0.3 3,148,939 + RR42_RS14635 0.80 +0.9 3,148,940 - RR42_RS14635 0.80 -0.2 3,149,081 - RR42_RS14635 0.90 -1.2 3,149,228 - -0.2 3,149,420 + +0.5 3,149,421 - -0.6 3,149,423 + -0.2 3,149,424 - +0.1 3,149,754 + RR42_RS14640 0.29 -0.3 3,149,755 - RR42_RS14640 0.29 -2.1 3,150,058 - RR42_RS14640 0.49 +0.3 3,150,077 + RR42_RS14640 0.50 +1.1 3,150,077 + RR42_RS14640 0.50 +0.0 3,150,078 - RR42_RS14640 0.50 +1.7 3,150,078 - RR42_RS14640 0.50 +0.5 3,150,184 + RR42_RS14640 0.57 +0.4 3,150,247 + RR42_RS14640 0.61 -0.8 3,150,248 - RR42_RS14640 0.61 +1.4 3,150,390 + RR42_RS14640 0.70 -1.1 3,150,528 + RR42_RS14640 0.79 -2.2 3,150,529 - RR42_RS14640 0.79 +0.2 3,150,788 + -1.1 3,150,789 - -0.3 3,151,414 + +0.2 3,151,414 + RR42_RS14645 0.34 +0.7 3,151,415 - RR42_RS14645 0.34 -0.4 3,151,415 - RR42_RS14645 0.34 -1.3 3,151,415 - RR42_RS14645 0.34 -1.0 3,151,489 + RR42_RS14645 0.38 +0.1 3,151,489 + RR42_RS14645 0.38 -0.7 3,151,489 + RR42_RS14645 0.38 +0.5 3,151,490 - RR42_RS14645 0.38 +3.4 3,151,490 - RR42_RS14645 0.38 -0.5 3,151,685 - RR42_RS14645 0.50 -0.2
Or see this region's nucleotide sequence