Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14360 and RR42_RS14365 are separated by 127 nucleotides RR42_RS14365 and RR42_RS14370 overlap by 17 nucleotides RR42_RS14370 and RR42_RS14375 are separated by 187 nucleotides
RR42_RS14360: RR42_RS14360 - tryptophan synthase subunit alpha, at 3,086,924 to 3,088,117
_RS14360
RR42_RS14365: RR42_RS14365 - N-(5'-phosphoribosyl)anthranilate isomerase, at 3,088,245 to 3,088,946
_RS14365
RR42_RS14370: RR42_RS14370 - tRNA pseudouridine synthase A, at 3,088,930 to 3,089,754
_RS14370
RR42_RS14375: RR42_RS14375 - pilus assembly protein FimV, at 3,089,942 to 3,092,824
_RS14375
Position (kb)
3088
3089
3090 Strain fitness (log2 ratio)
-2
-1
0
1 at 3088.017 kb on + strand at 3088.077 kb on + strand at 3088.335 kb on + strand, within RR42_RS14365 at 3088.335 kb on + strand, within RR42_RS14365 at 3088.710 kb on - strand at 3088.819 kb on + strand, within RR42_RS14365 at 3089.797 kb on - strand at 3089.929 kb on + strand at 3089.929 kb on + strand at 3089.929 kb on + strand at 3089.930 kb on - strand at 3090.055 kb on - strand at 3090.055 kb on - strand at 3090.055 kb on - strand at 3090.055 kb on - strand at 3090.356 kb on - strand, within RR42_RS14375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 3,088,017 + -2.3 3,088,077 + -1.1 3,088,335 + RR42_RS14365 0.13 -1.2 3,088,335 + RR42_RS14365 0.13 -2.7 3,088,710 - -0.3 3,088,819 + RR42_RS14365 0.82 -1.8 3,089,797 - -0.3 3,089,929 + -2.3 3,089,929 + -0.1 3,089,929 + +0.0 3,089,930 - -0.3 3,090,055 - -0.8 3,090,055 - -1.4 3,090,055 - +0.0 3,090,055 - -0.4 3,090,356 - RR42_RS14375 0.14 -0.3
Or see this region's nucleotide sequence