Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13140 and RR42_RS13145 are separated by 50 nucleotides RR42_RS13145 and RR42_RS13150 overlap by 8 nucleotides RR42_RS13150 and RR42_RS13155 overlap by 4 nucleotides
RR42_RS13140: RR42_RS13140 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 2,831,429 to 2,832,721
_RS13140
RR42_RS13145: RR42_RS13145 - DNA-binding protein, at 2,832,772 to 2,833,785
_RS13145
RR42_RS13150: RR42_RS13150 - pilus assembly protein PilW, at 2,833,778 to 2,834,557
_RS13150
RR42_RS13155: RR42_RS13155 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 2,834,554 to 2,835,708
_RS13155
Position (kb)
2832
2833
2834 Strain fitness (log2 ratio)
-2
-1
0
1 at 2832.728 kb on - strand at 2832.728 kb on - strand at 2832.895 kb on - strand, within RR42_RS13145 at 2833.791 kb on - strand at 2833.957 kb on - strand, within RR42_RS13150 at 2833.990 kb on - strand, within RR42_RS13150 at 2833.990 kb on - strand, within RR42_RS13150 at 2833.990 kb on - strand, within RR42_RS13150 at 2834.167 kb on - strand, within RR42_RS13150 at 2834.215 kb on - strand, within RR42_RS13150 at 2834.215 kb on - strand, within RR42_RS13150 at 2834.215 kb on - strand, within RR42_RS13150 at 2834.305 kb on - strand, within RR42_RS13150 at 2834.305 kb on - strand, within RR42_RS13150 at 2834.326 kb on - strand, within RR42_RS13150 at 2834.389 kb on - strand, within RR42_RS13150 at 2834.407 kb on - strand, within RR42_RS13150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,832,728 - -2.5 2,832,728 - -0.2 2,832,895 - RR42_RS13145 0.12 -1.4 2,833,791 - -0.9 2,833,957 - RR42_RS13150 0.23 +0.5 2,833,990 - RR42_RS13150 0.27 +1.5 2,833,990 - RR42_RS13150 0.27 -0.3 2,833,990 - RR42_RS13150 0.27 -2.0 2,834,167 - RR42_RS13150 0.50 -0.0 2,834,215 - RR42_RS13150 0.56 -0.5 2,834,215 - RR42_RS13150 0.56 -0.4 2,834,215 - RR42_RS13150 0.56 -0.4 2,834,305 - RR42_RS13150 0.68 +0.3 2,834,305 - RR42_RS13150 0.68 +0.7 2,834,326 - RR42_RS13150 0.70 -0.7 2,834,389 - RR42_RS13150 0.78 -1.1 2,834,407 - RR42_RS13150 0.81 +0.3
Or see this region's nucleotide sequence