Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06295 and RR42_RS06300 are separated by 165 nucleotides RR42_RS06300 and RR42_RS06305 are separated by 155 nucleotides RR42_RS06305 and RR42_RS06310 are separated by 154 nucleotides
RR42_RS06295: RR42_RS06295 - amino acid transporter, at 1,381,131 to 1,383,098
_RS06295
RR42_RS06300: RR42_RS06300 - DNA-binding protein, at 1,383,264 to 1,384,739
_RS06300
RR42_RS06305: RR42_RS06305 - hypothetical protein, at 1,384,895 to 1,385,530
_RS06305
RR42_RS06310: RR42_RS06310 - inositol monophosphatase, at 1,385,685 to 1,386,506
_RS06310
Position (kb)
1383
1384
1385 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1382.264 kb on - strand, within RR42_RS06295 at 1382.312 kb on - strand, within RR42_RS06295 at 1382.312 kb on - strand, within RR42_RS06295 at 1382.312 kb on - strand, within RR42_RS06295 at 1382.312 kb on - strand, within RR42_RS06295 at 1382.392 kb on + strand, within RR42_RS06295 at 1382.392 kb on + strand, within RR42_RS06295 at 1382.393 kb on - strand, within RR42_RS06295 at 1382.397 kb on - strand, within RR42_RS06295 at 1382.679 kb on + strand, within RR42_RS06295 at 1382.854 kb on + strand, within RR42_RS06295 at 1382.874 kb on + strand, within RR42_RS06295 at 1382.874 kb on + strand, within RR42_RS06295 at 1382.875 kb on - strand, within RR42_RS06295 at 1382.918 kb on + strand at 1382.919 kb on - strand at 1382.957 kb on - strand at 1383.214 kb on + strand at 1383.214 kb on + strand at 1383.214 kb on + strand at 1383.214 kb on + strand at 1383.215 kb on - strand at 1383.215 kb on - strand at 1383.215 kb on - strand at 1383.373 kb on + strand at 1383.373 kb on + strand at 1383.403 kb on + strand at 1383.403 kb on + strand at 1383.403 kb on + strand at 1383.404 kb on - strand at 1383.583 kb on + strand, within RR42_RS06300 at 1383.584 kb on - strand, within RR42_RS06300 at 1383.715 kb on + strand, within RR42_RS06300 at 1383.715 kb on + strand, within RR42_RS06300 at 1383.781 kb on + strand, within RR42_RS06300 at 1383.968 kb on - strand, within RR42_RS06300 at 1383.968 kb on - strand, within RR42_RS06300 at 1383.968 kb on - strand, within RR42_RS06300 at 1384.223 kb on - strand, within RR42_RS06300 at 1384.546 kb on + strand, within RR42_RS06300 at 1384.552 kb on + strand, within RR42_RS06300 at 1384.552 kb on + strand, within RR42_RS06300 at 1384.552 kb on + strand, within RR42_RS06300 at 1384.715 kb on + strand at 1384.776 kb on + strand at 1384.824 kb on - strand at 1384.962 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.081 kb on + strand, within RR42_RS06305 at 1385.081 kb on + strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.207 kb on + strand, within RR42_RS06305 at 1385.207 kb on + strand, within RR42_RS06305 at 1385.225 kb on + strand, within RR42_RS06305 at 1385.226 kb on - strand, within RR42_RS06305 at 1385.226 kb on - strand, within RR42_RS06305 at 1385.516 kb on + strand at 1385.517 kb on - strand at 1385.517 kb on - strand at 1385.517 kb on - strand at 1385.686 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 1,382,264 - RR42_RS06295 0.58 -0.8 1,382,312 - RR42_RS06295 0.60 +1.0 1,382,312 - RR42_RS06295 0.60 +0.1 1,382,312 - RR42_RS06295 0.60 -0.6 1,382,312 - RR42_RS06295 0.60 -1.7 1,382,392 + RR42_RS06295 0.64 +0.1 1,382,392 + RR42_RS06295 0.64 +1.2 1,382,393 - RR42_RS06295 0.64 +0.3 1,382,397 - RR42_RS06295 0.64 -0.3 1,382,679 + RR42_RS06295 0.79 -0.7 1,382,854 + RR42_RS06295 0.88 +0.7 1,382,874 + RR42_RS06295 0.89 -0.1 1,382,874 + RR42_RS06295 0.89 -0.5 1,382,875 - RR42_RS06295 0.89 -0.3 1,382,918 + -1.3 1,382,919 - -0.1 1,382,957 - +0.1 1,383,214 + -0.5 1,383,214 + +0.2 1,383,214 + +0.1 1,383,214 + +0.1 1,383,215 - -0.6 1,383,215 - +0.8 1,383,215 - +0.4 1,383,373 + -0.8 1,383,373 + -0.2 1,383,403 + -0.6 1,383,403 + +0.3 1,383,403 + -0.2 1,383,404 - -0.0 1,383,583 + RR42_RS06300 0.22 -0.0 1,383,584 - RR42_RS06300 0.22 +0.1 1,383,715 + RR42_RS06300 0.31 -0.0 1,383,715 + RR42_RS06300 0.31 +0.8 1,383,781 + RR42_RS06300 0.35 -0.8 1,383,968 - RR42_RS06300 0.48 -2.3 1,383,968 - RR42_RS06300 0.48 +1.3 1,383,968 - RR42_RS06300 0.48 +0.1 1,384,223 - RR42_RS06300 0.65 +0.4 1,384,546 + RR42_RS06300 0.87 -0.1 1,384,552 + RR42_RS06300 0.87 +0.1 1,384,552 + RR42_RS06300 0.87 +0.1 1,384,552 + RR42_RS06300 0.87 -0.3 1,384,715 + +0.3 1,384,776 + -2.5 1,384,824 - +0.5 1,384,962 - RR42_RS06305 0.11 +0.4 1,385,001 - RR42_RS06305 0.17 -0.5 1,385,001 - RR42_RS06305 0.17 +0.2 1,385,001 - RR42_RS06305 0.17 +0.4 1,385,001 - RR42_RS06305 0.17 +1.3 1,385,081 + RR42_RS06305 0.29 +0.2 1,385,081 + RR42_RS06305 0.29 +0.6 1,385,082 - RR42_RS06305 0.29 -0.7 1,385,082 - RR42_RS06305 0.29 +1.8 1,385,082 - RR42_RS06305 0.29 +0.4 1,385,082 - RR42_RS06305 0.29 +0.7 1,385,082 - RR42_RS06305 0.29 +0.3 1,385,207 + RR42_RS06305 0.49 +0.9 1,385,207 + RR42_RS06305 0.49 -0.0 1,385,225 + RR42_RS06305 0.52 -0.2 1,385,226 - RR42_RS06305 0.52 +1.3 1,385,226 - RR42_RS06305 0.52 +0.2 1,385,516 + -0.9 1,385,517 - -0.6 1,385,517 - +0.4 1,385,517 - +0.1 1,385,686 + -0.9
Or see this region's nucleotide sequence