Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04970 and RR42_RS04975 are separated by 88 nucleotides RR42_RS04975 and RR42_RS04980 are separated by 206 nucleotides RR42_RS04980 and RR42_RS04985 are separated by 172 nucleotides
RR42_RS04970: RR42_RS04970 - aspartyl beta-hydroxylase, at 1,098,115 to 1,099,017
_RS04970
RR42_RS04975: RR42_RS04975 - histidine kinase, at 1,099,106 to 1,100,581
_RS04975
RR42_RS04980: RR42_RS04980 - hypothetical protein, at 1,100,788 to 1,101,189
_RS04980
RR42_RS04985: RR42_RS04985 - 2-aminoadipate aminotransferase, at 1,101,362 to 1,102,804
_RS04985
Position (kb)
1099
1100
1101 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1098.160 kb on + strand at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.254 kb on + strand, within RR42_RS04970 at 1098.255 kb on - strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.428 kb on - strand, within RR42_RS04970 at 1098.445 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on - strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.716 kb on - strand, within RR42_RS04970 at 1098.901 kb on - strand, within RR42_RS04970 at 1098.959 kb on - strand at 1098.961 kb on + strand at 1098.961 kb on + strand at 1098.962 kb on - strand at 1098.962 kb on - strand at 1098.962 kb on - strand at 1099.031 kb on + strand at 1099.032 kb on - strand at 1099.032 kb on - strand at 1099.032 kb on - strand at 1099.146 kb on + strand at 1099.147 kb on - strand at 1099.168 kb on - strand at 1099.168 kb on - strand at 1099.168 kb on - strand at 1099.168 kb on - strand at 1099.264 kb on - strand, within RR42_RS04975 at 1099.312 kb on + strand, within RR42_RS04975 at 1099.343 kb on + strand, within RR42_RS04975 at 1099.343 kb on + strand, within RR42_RS04975 at 1099.343 kb on + strand, within RR42_RS04975 at 1099.344 kb on - strand, within RR42_RS04975 at 1099.344 kb on - strand, within RR42_RS04975 at 1099.552 kb on - strand, within RR42_RS04975 at 1099.555 kb on - strand, within RR42_RS04975 at 1099.567 kb on - strand, within RR42_RS04975 at 1099.567 kb on - strand, within RR42_RS04975 at 1099.608 kb on + strand, within RR42_RS04975 at 1099.608 kb on + strand, within RR42_RS04975 at 1099.608 kb on + strand, within RR42_RS04975 at 1099.609 kb on - strand, within RR42_RS04975 at 1099.609 kb on - strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.706 kb on + strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.707 kb on - strand, within RR42_RS04975 at 1099.811 kb on - strand, within RR42_RS04975 at 1099.811 kb on - strand, within RR42_RS04975 at 1100.067 kb on + strand, within RR42_RS04975 at 1100.068 kb on - strand, within RR42_RS04975 at 1100.068 kb on - strand, within RR42_RS04975 at 1100.214 kb on + strand, within RR42_RS04975 at 1100.214 kb on + strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.215 kb on - strand, within RR42_RS04975 at 1100.290 kb on + strand, within RR42_RS04975 at 1100.291 kb on - strand, within RR42_RS04975 at 1100.318 kb on - strand, within RR42_RS04975 at 1100.544 kb on + strand at 1100.544 kb on + strand at 1100.544 kb on + strand at 1100.545 kb on - strand at 1100.716 kb on + strand at 1100.717 kb on - strand at 1100.717 kb on - strand at 1100.721 kb on + strand at 1100.740 kb on - strand at 1100.740 kb on - strand at 1100.745 kb on + strand at 1100.746 kb on - strand at 1101.000 kb on + strand, within RR42_RS04980 at 1101.001 kb on - strand, within RR42_RS04980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 1,098,160 + +0.7 1,098,250 + RR42_RS04970 0.15 -0.6 1,098,250 + RR42_RS04970 0.15 +0.3 1,098,250 + RR42_RS04970 0.15 +1.3 1,098,250 + RR42_RS04970 0.15 -3.4 1,098,250 + RR42_RS04970 0.15 +0.2 1,098,251 - RR42_RS04970 0.15 +0.5 1,098,251 - RR42_RS04970 0.15 +0.9 1,098,251 - RR42_RS04970 0.15 +0.5 1,098,251 - RR42_RS04970 0.15 +0.6 1,098,251 - RR42_RS04970 0.15 -0.5 1,098,254 + RR42_RS04970 0.15 -2.4 1,098,255 - RR42_RS04970 0.16 +0.1 1,098,280 + RR42_RS04970 0.18 +0.5 1,098,280 + RR42_RS04970 0.18 -0.4 1,098,280 + RR42_RS04970 0.18 +0.1 1,098,281 - RR42_RS04970 0.18 +1.5 1,098,281 - RR42_RS04970 0.18 +0.8 1,098,281 - RR42_RS04970 0.18 -0.7 1,098,281 - RR42_RS04970 0.18 +0.2 1,098,428 - RR42_RS04970 0.35 -0.0 1,098,445 + RR42_RS04970 0.37 -0.3 1,098,675 + RR42_RS04970 0.62 +1.2 1,098,675 + RR42_RS04970 0.62 +0.3 1,098,675 + RR42_RS04970 0.62 -1.2 1,098,676 - RR42_RS04970 0.62 -0.0 1,098,676 - RR42_RS04970 0.62 -0.0 1,098,676 - RR42_RS04970 0.62 +1.1 1,098,701 + RR42_RS04970 0.65 -1.1 1,098,701 + RR42_RS04970 0.65 -0.1 1,098,701 - RR42_RS04970 0.65 -0.0 1,098,715 + RR42_RS04970 0.66 -0.6 1,098,715 + RR42_RS04970 0.66 -0.6 1,098,716 - RR42_RS04970 0.67 +0.5 1,098,901 - RR42_RS04970 0.87 -1.0 1,098,959 - -1.5 1,098,961 + -0.1 1,098,961 + +0.0 1,098,962 - +0.9 1,098,962 - +0.1 1,098,962 - -0.0 1,099,031 + +1.4 1,099,032 - +0.2 1,099,032 - +0.5 1,099,032 - +1.2 1,099,146 + -1.6 1,099,147 - -0.2 1,099,168 - -1.8 1,099,168 - -2.2 1,099,168 - +0.5 1,099,168 - -1.4 1,099,264 - RR42_RS04975 0.11 +0.3 1,099,312 + RR42_RS04975 0.14 -0.5 1,099,343 + RR42_RS04975 0.16 -0.2 1,099,343 + RR42_RS04975 0.16 +0.1 1,099,343 + RR42_RS04975 0.16 -1.8 1,099,344 - RR42_RS04975 0.16 -0.2 1,099,344 - RR42_RS04975 0.16 -0.4 1,099,552 - RR42_RS04975 0.30 -1.3 1,099,555 - RR42_RS04975 0.30 -1.0 1,099,567 - RR42_RS04975 0.31 +0.1 1,099,567 - RR42_RS04975 0.31 -0.2 1,099,608 + RR42_RS04975 0.34 +0.4 1,099,608 + RR42_RS04975 0.34 -0.3 1,099,608 + RR42_RS04975 0.34 +0.1 1,099,609 - RR42_RS04975 0.34 -0.7 1,099,609 - RR42_RS04975 0.34 +0.3 1,099,706 + RR42_RS04975 0.41 -0.3 1,099,706 + RR42_RS04975 0.41 -1.2 1,099,706 + RR42_RS04975 0.41 +1.1 1,099,706 + RR42_RS04975 0.41 +0.3 1,099,707 - RR42_RS04975 0.41 +0.0 1,099,707 - RR42_RS04975 0.41 +0.4 1,099,707 - RR42_RS04975 0.41 -1.2 1,099,707 - RR42_RS04975 0.41 -1.3 1,099,707 - RR42_RS04975 0.41 +0.8 1,099,707 - RR42_RS04975 0.41 -0.8 1,099,811 - RR42_RS04975 0.48 -0.2 1,099,811 - RR42_RS04975 0.48 -0.8 1,100,067 + RR42_RS04975 0.65 +0.4 1,100,068 - RR42_RS04975 0.65 +0.1 1,100,068 - RR42_RS04975 0.65 +0.3 1,100,214 + RR42_RS04975 0.75 +0.9 1,100,214 + RR42_RS04975 0.75 +0.4 1,100,215 - RR42_RS04975 0.75 +0.5 1,100,215 - RR42_RS04975 0.75 -2.5 1,100,215 - RR42_RS04975 0.75 -0.1 1,100,215 - RR42_RS04975 0.75 +0.9 1,100,215 - RR42_RS04975 0.75 +1.8 1,100,290 + RR42_RS04975 0.80 -0.1 1,100,291 - RR42_RS04975 0.80 +0.4 1,100,318 - RR42_RS04975 0.82 -0.3 1,100,544 + -0.8 1,100,544 + +0.6 1,100,544 + -0.6 1,100,545 - -0.7 1,100,716 + +0.1 1,100,717 - -0.2 1,100,717 - +0.8 1,100,721 + -0.1 1,100,740 - +0.2 1,100,740 - -1.1 1,100,745 + -0.0 1,100,746 - +1.0 1,101,000 + RR42_RS04980 0.53 -0.9 1,101,001 - RR42_RS04980 0.53 -1.1
Or see this region's nucleotide sequence