Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04280 and RR42_RS04285 are separated by 189 nucleotides RR42_RS04285 and RR42_RS04290 are separated by 180 nucleotides RR42_RS04290 and RR42_RS04295 are separated by 29 nucleotides
RR42_RS04280: RR42_RS04280 - aldehyde dehydrogenase, at 951,613 to 953,049
_RS04280
RR42_RS04285: RR42_RS04285 - inorganic pyrophosphatase, at 953,239 to 953,766
_RS04285
RR42_RS04290: RR42_RS04290 - cytochrome B561, at 953,947 to 954,501
_RS04290
RR42_RS04295: RR42_RS04295 - hypothetical protein, at 954,531 to 955,790
_RS04295
Position (kb) 953
954
955 Strain fitness (log2 ratio) -2
-1
0
1 at 952.984 kb on + strand at 952.984 kb on + strand at 953.054 kb on + strand at 953.195 kb on + strand at 953.195 kb on + strand at 953.196 kb on - strand at 953.196 kb on - strand at 953.764 kb on + strand at 953.764 kb on + strand at 953.765 kb on - strand at 953.886 kb on + strand at 953.900 kb on + strand at 953.939 kb on + strand at 953.939 kb on + strand at 953.955 kb on + strand at 953.955 kb on + strand at 953.955 kb on + strand at 953.986 kb on + strand at 954.020 kb on + strand, within RR42_RS04290 at 954.020 kb on + strand, within RR42_RS04290 at 954.021 kb on - strand, within RR42_RS04290 at 954.021 kb on - strand, within RR42_RS04290 at 954.043 kb on + strand, within RR42_RS04290 at 954.242 kb on + strand, within RR42_RS04290 at 954.242 kb on + strand, within RR42_RS04290 at 954.242 kb on + strand, within RR42_RS04290 at 954.243 kb on - strand, within RR42_RS04290 at 954.247 kb on + strand, within RR42_RS04290 at 954.247 kb on + strand, within RR42_RS04290 at 954.248 kb on - strand, within RR42_RS04290 at 954.257 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.415 kb on + strand, within RR42_RS04290 at 954.416 kb on - strand, within RR42_RS04290 at 954.460 kb on + strand at 954.460 kb on + strand at 954.460 kb on + strand at 954.461 kb on - strand at 954.461 kb on - strand at 954.461 kb on - strand at 954.475 kb on - strand at 954.499 kb on + strand at 954.574 kb on + strand at 954.723 kb on - strand, within RR42_RS04295 at 954.760 kb on + strand, within RR42_RS04295 at 954.761 kb on - strand, within RR42_RS04295 at 954.763 kb on + strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.764 kb on - strand, within RR42_RS04295 at 954.812 kb on - strand, within RR42_RS04295 at 954.851 kb on - strand, within RR42_RS04295 at 954.851 kb on - strand, within RR42_RS04295 at 954.995 kb on - strand, within RR42_RS04295 at 955.385 kb on - strand, within RR42_RS04295 at 955.406 kb on - strand, within RR42_RS04295 at 955.406 kb on - strand, within RR42_RS04295 at 955.435 kb on + strand, within RR42_RS04295 at 955.435 kb on + strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295 at 955.436 kb on - strand, within RR42_RS04295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 952,984 + -0.8 952,984 + +0.3 953,054 + -0.3 953,195 + +0.3 953,195 + +0.4 953,196 - +0.3 953,196 - -0.8 953,764 + +0.1 953,764 + +0.6 953,765 - +0.0 953,886 + -0.5 953,900 + -0.2 953,939 + -0.7 953,939 + +0.3 953,955 + -1.1 953,955 + +1.0 953,955 + -1.2 953,986 + -0.1 954,020 + RR42_RS04290 0.13 +0.1 954,020 + RR42_RS04290 0.13 -0.6 954,021 - RR42_RS04290 0.13 -1.5 954,021 - RR42_RS04290 0.13 -1.1 954,043 + RR42_RS04290 0.17 +0.8 954,242 + RR42_RS04290 0.53 -2.4 954,242 + RR42_RS04290 0.53 -0.1 954,242 + RR42_RS04290 0.53 +0.4 954,243 - RR42_RS04290 0.53 +0.5 954,247 + RR42_RS04290 0.54 -2.0 954,247 + RR42_RS04290 0.54 -0.7 954,248 - RR42_RS04290 0.54 +0.3 954,257 + RR42_RS04290 0.56 +0.6 954,415 + RR42_RS04290 0.84 -0.0 954,415 + RR42_RS04290 0.84 -0.8 954,415 + RR42_RS04290 0.84 +0.3 954,415 + RR42_RS04290 0.84 +0.0 954,415 + RR42_RS04290 0.84 -0.1 954,415 + RR42_RS04290 0.84 -0.8 954,415 + RR42_RS04290 0.84 +0.1 954,416 - RR42_RS04290 0.85 -0.6 954,460 + +0.2 954,460 + -2.4 954,460 + +0.2 954,461 - +0.3 954,461 - -0.9 954,461 - +0.2 954,475 - -0.4 954,499 + -0.2 954,574 + -0.3 954,723 - RR42_RS04295 0.15 +0.9 954,760 + RR42_RS04295 0.18 +0.3 954,761 - RR42_RS04295 0.18 +0.3 954,763 + RR42_RS04295 0.18 +0.7 954,764 - RR42_RS04295 0.18 +0.5 954,764 - RR42_RS04295 0.18 +0.8 954,764 - RR42_RS04295 0.18 -0.3 954,764 - RR42_RS04295 0.18 -0.4 954,764 - RR42_RS04295 0.18 -0.3 954,812 - RR42_RS04295 0.22 +0.1 954,851 - RR42_RS04295 0.25 +0.1 954,851 - RR42_RS04295 0.25 +0.3 954,995 - RR42_RS04295 0.37 -0.5 955,385 - RR42_RS04295 0.68 -0.1 955,406 - RR42_RS04295 0.69 -0.2 955,406 - RR42_RS04295 0.69 -0.7 955,435 + RR42_RS04295 0.72 +0.3 955,435 + RR42_RS04295 0.72 +0.2 955,436 - RR42_RS04295 0.72 +0.5 955,436 - RR42_RS04295 0.72 -0.2 955,436 - RR42_RS04295 0.72 +0.3 955,436 - RR42_RS04295 0.72 -0.6
Or see this region's nucleotide sequence