Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03905 and RR42_RS03910 are separated by 169 nucleotides RR42_RS03910 and RR42_RS03915 overlap by 4 nucleotides
RR42_RS03905: RR42_RS03905 - hypothetical protein, at 860,946 to 861,863
_RS03905
RR42_RS03910: RR42_RS03910 - (2Fe-2S)-binding protein, at 862,033 to 862,506
_RS03910
RR42_RS03915: RR42_RS03915 - hypothetical protein, at 862,503 to 864,788
_RS03915
Position (kb)
862
863 Strain fitness (log2 ratio)
-1
0
1
2 at 861.064 kb on + strand, within RR42_RS03905 at 861.281 kb on + strand, within RR42_RS03905 at 861.281 kb on + strand, within RR42_RS03905 at 861.282 kb on - strand, within RR42_RS03905 at 861.282 kb on - strand, within RR42_RS03905 at 861.282 kb on - strand, within RR42_RS03905 at 861.539 kb on + strand, within RR42_RS03905 at 861.556 kb on + strand, within RR42_RS03905 at 861.556 kb on + strand, within RR42_RS03905 at 861.556 kb on + strand, within RR42_RS03905 at 861.556 kb on + strand, within RR42_RS03905 at 861.556 kb on + strand, within RR42_RS03905 at 861.557 kb on - strand, within RR42_RS03905 at 861.590 kb on - strand, within RR42_RS03905 at 861.784 kb on - strand at 861.785 kb on + strand at 861.785 kb on + strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.786 kb on - strand at 861.889 kb on + strand at 862.441 kb on + strand, within RR42_RS03910 at 862.442 kb on - strand, within RR42_RS03910 at 862.442 kb on - strand, within RR42_RS03910 at 862.523 kb on + strand at 862.524 kb on - strand at 862.737 kb on + strand, within RR42_RS03915 at 862.738 kb on - strand, within RR42_RS03915 at 862.814 kb on - strand, within RR42_RS03915 at 862.814 kb on - strand, within RR42_RS03915 at 863.052 kb on + strand, within RR42_RS03915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 861,064 + RR42_RS03905 0.13 +0.7 861,281 + RR42_RS03905 0.36 +0.4 861,281 + RR42_RS03905 0.36 -0.1 861,282 - RR42_RS03905 0.37 +0.9 861,282 - RR42_RS03905 0.37 -0.7 861,282 - RR42_RS03905 0.37 +0.3 861,539 + RR42_RS03905 0.65 +0.5 861,556 + RR42_RS03905 0.66 +0.1 861,556 + RR42_RS03905 0.66 +2.5 861,556 + RR42_RS03905 0.66 +0.3 861,556 + RR42_RS03905 0.66 +0.9 861,556 + RR42_RS03905 0.66 +0.2 861,557 - RR42_RS03905 0.67 -0.1 861,590 - RR42_RS03905 0.70 -0.1 861,784 - +2.0 861,785 + +1.2 861,785 + +1.1 861,786 - +0.2 861,786 - -1.0 861,786 - +1.7 861,786 - +1.5 861,786 - -0.4 861,786 - -0.1 861,786 - -0.1 861,786 - -0.1 861,889 + +0.9 862,441 + RR42_RS03910 0.86 +2.4 862,442 - RR42_RS03910 0.86 +1.9 862,442 - RR42_RS03910 0.86 +0.4 862,523 + -0.5 862,524 - +0.2 862,737 + RR42_RS03915 0.10 -0.3 862,738 - RR42_RS03915 0.10 +0.6 862,814 - RR42_RS03915 0.14 +1.2 862,814 - RR42_RS03915 0.14 +0.9 863,052 + RR42_RS03915 0.24 -0.1
Or see this region's nucleotide sequence