Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03280 and RR42_RS03285 are separated by 57 nucleotides RR42_RS03285 and RR42_RS03290 are separated by 146 nucleotides RR42_RS03290 and RR42_RS03295 overlap by 8 nucleotides
RR42_RS03280: RR42_RS03280 - LysR family transcriptional regulator, at 725,760 to 726,665
_RS03280
RR42_RS03285: RR42_RS03285 - tricarboxylic transport membrane protein, at 726,723 to 727,679
_RS03285
RR42_RS03290: RR42_RS03290 - transcriptional regulator, at 727,826 to 728,527
_RS03290
RR42_RS03295: RR42_RS03295 - histidine kinase, at 728,520 to 729,947
_RS03295
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-2
-1
0
1 at 726.067 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.663 kb on + strand at 726.664 kb on - strand at 726.698 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.788 kb on + strand at 726.788 kb on + strand at 726.898 kb on + strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.928 kb on + strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.166 kb on - strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.320 kb on + strand, within RR42_RS03285 at 727.331 kb on - strand, within RR42_RS03285 at 727.375 kb on + strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.475 kb on - strand, within RR42_RS03285 at 727.505 kb on - strand, within RR42_RS03285 at 727.789 kb on - strand at 727.789 kb on - strand at 727.833 kb on + strand at 727.833 kb on + strand at 727.833 kb on + strand at 727.834 kb on + strand at 727.834 kb on - strand at 727.988 kb on + strand at 727.989 kb on - strand, within RR42_RS03290 at 728.147 kb on + strand, within RR42_RS03290 at 728.147 kb on + strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.334 kb on + strand, within RR42_RS03290 at 728.335 kb on - strand, within RR42_RS03290 at 728.335 kb on - strand, within RR42_RS03290 at 728.378 kb on + strand, within RR42_RS03290 at 728.379 kb on - strand, within RR42_RS03290 at 728.432 kb on + strand, within RR42_RS03290 at 728.433 kb on - strand, within RR42_RS03290 at 728.502 kb on - strand at 728.502 kb on - strand at 728.502 kb on - strand at 728.655 kb on + strand at 728.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 726,067 - RR42_RS03280 0.34 +0.7 726,194 - RR42_RS03280 0.48 +0.4 726,194 - RR42_RS03280 0.48 -0.3 726,194 - RR42_RS03280 0.48 -1.1 726,194 - RR42_RS03280 0.48 -0.8 726,663 + +0.2 726,664 - +0.4 726,698 + -0.1 726,784 + -1.1 726,784 + -0.4 726,784 + -1.4 726,784 + -0.6 726,784 + -1.1 726,784 + -0.5 726,784 + -0.1 726,784 + +0.6 726,784 + -0.4 726,784 + +0.0 726,784 + +0.1 726,785 - -0.1 726,785 - -0.2 726,785 - -0.3 726,785 - -0.1 726,785 - +0.1 726,785 - -0.3 726,785 - -0.4 726,785 - -1.7 726,785 - +0.9 726,785 - -1.5 726,788 + +0.3 726,788 + -0.8 726,898 + RR42_RS03285 0.18 +0.9 726,899 - RR42_RS03285 0.18 +1.1 726,899 - RR42_RS03285 0.18 -0.2 726,928 + RR42_RS03285 0.21 -1.7 726,929 - RR42_RS03285 0.22 +0.3 726,929 - RR42_RS03285 0.22 -0.5 727,165 + RR42_RS03285 0.46 -0.2 727,165 + RR42_RS03285 0.46 -0.1 727,166 - RR42_RS03285 0.46 +0.9 727,245 + RR42_RS03285 0.55 +1.2 727,245 + RR42_RS03285 0.55 -0.7 727,245 + RR42_RS03285 0.55 -0.5 727,245 + RR42_RS03285 0.55 -0.3 727,246 - RR42_RS03285 0.55 +0.6 727,246 - RR42_RS03285 0.55 -0.4 727,246 - RR42_RS03285 0.55 -0.3 727,320 + RR42_RS03285 0.62 -0.9 727,331 - RR42_RS03285 0.64 -0.8 727,375 + RR42_RS03285 0.68 -0.5 727,376 - RR42_RS03285 0.68 -0.8 727,376 - RR42_RS03285 0.68 +0.6 727,474 + RR42_RS03285 0.78 +0.1 727,474 + RR42_RS03285 0.78 +0.4 727,474 + RR42_RS03285 0.78 -0.8 727,474 + RR42_RS03285 0.78 -0.4 727,475 - RR42_RS03285 0.79 +0.4 727,505 - RR42_RS03285 0.82 -1.2 727,789 - -0.3 727,789 - -0.4 727,833 + -0.1 727,833 + -0.3 727,833 + -0.7 727,834 + +0.0 727,834 - -1.8 727,988 + -1.1 727,989 - RR42_RS03290 0.23 -0.7 728,147 + RR42_RS03290 0.46 -0.8 728,147 + RR42_RS03290 0.46 -0.4 728,148 - RR42_RS03290 0.46 +0.6 728,148 - RR42_RS03290 0.46 -0.9 728,148 - RR42_RS03290 0.46 +0.2 728,148 - RR42_RS03290 0.46 +0.0 728,334 + RR42_RS03290 0.72 -0.2 728,335 - RR42_RS03290 0.73 -0.6 728,335 - RR42_RS03290 0.73 +0.9 728,378 + RR42_RS03290 0.79 -1.0 728,379 - RR42_RS03290 0.79 +0.4 728,432 + RR42_RS03290 0.86 -0.9 728,433 - RR42_RS03290 0.86 -0.3 728,502 - +0.9 728,502 - +0.3 728,502 - -0.3 728,655 + -0.4 728,656 - -1.7
Or see this region's nucleotide sequence