Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02905 and RR42_RS02910 are separated by 34 nucleotides RR42_RS02910 and RR42_RS02915 are separated by 220 nucleotides
RR42_RS02905: RR42_RS02905 - MFS transporter, at 647,931 to 648,929
_RS02905
RR42_RS02910: RR42_RS02910 - 2-aminoadipate aminotransferase, at 648,964 to 650,214
_RS02910
RR42_RS02915: RR42_RS02915 - hypothetical protein, at 650,435 to 651,466
_RS02915
Position (kb) 648
649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 648.024 kb on - strand at 648.201 kb on + strand, within RR42_RS02905 at 648.201 kb on + strand, within RR42_RS02905 at 648.252 kb on + strand, within RR42_RS02905 at 648.388 kb on - strand, within RR42_RS02905 at 648.504 kb on + strand, within RR42_RS02905 at 648.504 kb on + strand, within RR42_RS02905 at 648.504 kb on + strand, within RR42_RS02905 at 648.505 kb on - strand, within RR42_RS02905 at 648.566 kb on + strand, within RR42_RS02905 at 648.624 kb on - strand, within RR42_RS02905 at 649.045 kb on + strand at 649.046 kb on - strand at 649.171 kb on + strand, within RR42_RS02910 at 649.690 kb on + strand, within RR42_RS02910 at 649.888 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.248 kb on - strand at 650.364 kb on + strand at 650.364 kb on + strand at 650.365 kb on - strand at 650.365 kb on - strand at 650.368 kb on + strand at 650.368 kb on + strand at 650.369 kb on - strand at 650.369 kb on - strand at 650.369 kb on - strand at 650.403 kb on + strand at 650.426 kb on + strand at 650.682 kb on - strand, within RR42_RS02915 at 650.682 kb on - strand, within RR42_RS02915 at 650.682 kb on - strand, within RR42_RS02915 at 650.750 kb on - strand, within RR42_RS02915 at 650.788 kb on + strand, within RR42_RS02915 at 650.788 kb on + strand, within RR42_RS02915 at 650.788 kb on + strand, within RR42_RS02915 at 650.966 kb on + strand, within RR42_RS02915 at 650.966 kb on + strand, within RR42_RS02915 at 650.967 kb on - strand, within RR42_RS02915 at 650.976 kb on + strand, within RR42_RS02915 at 651.056 kb on + strand, within RR42_RS02915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 648,024 - -0.5 648,201 + RR42_RS02905 0.27 +0.3 648,201 + RR42_RS02905 0.27 +0.4 648,252 + RR42_RS02905 0.32 -0.1 648,388 - RR42_RS02905 0.46 -1.3 648,504 + RR42_RS02905 0.57 -1.6 648,504 + RR42_RS02905 0.57 +0.4 648,504 + RR42_RS02905 0.57 -0.8 648,505 - RR42_RS02905 0.57 -1.4 648,566 + RR42_RS02905 0.64 -0.9 648,624 - RR42_RS02905 0.69 +0.3 649,045 + +0.8 649,046 - -0.4 649,171 + RR42_RS02910 0.17 -0.7 649,690 + RR42_RS02910 0.58 +0.4 649,888 + RR42_RS02910 0.74 -0.1 650,050 + RR42_RS02910 0.87 +0.2 650,050 + RR42_RS02910 0.87 +0.1 650,050 + RR42_RS02910 0.87 -0.3 650,050 + RR42_RS02910 0.87 -0.9 650,050 + RR42_RS02910 0.87 +0.1 650,050 + RR42_RS02910 0.87 -0.1 650,050 + RR42_RS02910 0.87 -0.7 650,051 - RR42_RS02910 0.87 -0.8 650,051 - RR42_RS02910 0.87 -1.9 650,051 - RR42_RS02910 0.87 +0.0 650,051 - RR42_RS02910 0.87 +0.0 650,051 - RR42_RS02910 0.87 +0.2 650,051 - RR42_RS02910 0.87 -3.0 650,248 - -0.2 650,364 + +0.5 650,364 + -0.4 650,365 - -0.6 650,365 - -0.7 650,368 + +0.2 650,368 + -0.5 650,369 - +0.3 650,369 - +0.2 650,369 - -1.8 650,403 + -1.6 650,426 + +0.4 650,682 - RR42_RS02915 0.24 +1.0 650,682 - RR42_RS02915 0.24 +0.5 650,682 - RR42_RS02915 0.24 +0.3 650,750 - RR42_RS02915 0.31 +0.3 650,788 + RR42_RS02915 0.34 -0.1 650,788 + RR42_RS02915 0.34 -1.5 650,788 + RR42_RS02915 0.34 +0.9 650,966 + RR42_RS02915 0.51 -1.0 650,966 + RR42_RS02915 0.51 -0.9 650,967 - RR42_RS02915 0.52 -0.0 650,976 + RR42_RS02915 0.52 +0.4 651,056 + RR42_RS02915 0.60 -0.0
Or see this region's nucleotide sequence