Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01185 and RR42_RS01190 are separated by 246 nucleotides RR42_RS01190 and RR42_RS01195 are separated by 75 nucleotides RR42_RS01195 and RR42_RS01200 are separated by 64 nucleotides
RR42_RS01185: RR42_RS01185 - muropeptide transporter, at 271,272 to 272,528
_RS01185
RR42_RS01190: RR42_RS01190 - peptidase M48, at 272,775 to 273,722
_RS01190
RR42_RS01195: RR42_RS01195 - excinuclease ATPase, at 273,798 to 274,271
_RS01195
RR42_RS01200: RR42_RS01200 - hypothetical protein, at 274,336 to 275,145
_RS01200
Position (kb) 272
273
274 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 271.826 kb on - strand, within RR42_RS01185 at 271.996 kb on + strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 272.134 kb on + strand, within RR42_RS01185 at 272.135 kb on - strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.167 kb on - strand, within RR42_RS01185 at 272.309 kb on + strand, within RR42_RS01185 at 272.310 kb on - strand, within RR42_RS01185 at 272.312 kb on - strand, within RR42_RS01185 at 272.312 kb on - strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.513 kb on - strand at 272.604 kb on - strand at 272.604 kb on - strand at 272.607 kb on - strand at 272.607 kb on - strand at 272.607 kb on - strand at 272.615 kb on - strand at 272.615 kb on - strand at 272.687 kb on - strand at 272.958 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.169 kb on - strand, within RR42_RS01190 at 273.344 kb on + strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand at 273.345 kb on - strand, within RR42_RS01190 at 273.447 kb on + strand, within RR42_RS01190 at 273.653 kb on + strand at 273.713 kb on - strand at 273.720 kb on + strand at 273.759 kb on - strand at 273.759 kb on - strand at 273.763 kb on + strand at 273.763 kb on + strand at 273.764 kb on - strand at 273.764 kb on - strand at 273.767 kb on + strand at 273.767 kb on + strand at 274.200 kb on + strand, within RR42_RS01195 at 274.200 kb on + strand, within RR42_RS01195 at 274.201 kb on - strand, within RR42_RS01195 at 274.224 kb on + strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 271,826 - RR42_RS01185 0.44 -0.1 271,996 + RR42_RS01185 0.58 -1.5 271,997 - RR42_RS01185 0.58 -0.6 271,997 - RR42_RS01185 0.58 -0.0 272,134 + RR42_RS01185 0.69 -0.8 272,135 - RR42_RS01185 0.69 -2.2 272,166 + RR42_RS01185 0.71 -0.5 272,166 + RR42_RS01185 0.71 -0.3 272,166 + RR42_RS01185 0.71 -3.4 272,167 - RR42_RS01185 0.71 -0.3 272,309 + RR42_RS01185 0.82 -0.6 272,310 - RR42_RS01185 0.83 -1.7 272,312 - RR42_RS01185 0.83 -0.1 272,312 - RR42_RS01185 0.83 -1.0 272,348 + RR42_RS01185 0.86 -1.2 272,348 + RR42_RS01185 0.86 -1.1 272,348 + RR42_RS01185 0.86 -0.3 272,349 - RR42_RS01185 0.86 -0.7 272,349 - RR42_RS01185 0.86 +0.4 272,349 - RR42_RS01185 0.86 -1.9 272,349 - RR42_RS01185 0.86 -0.7 272,349 - RR42_RS01185 0.86 -0.4 272,350 + RR42_RS01185 0.86 +0.0 272,350 + RR42_RS01185 0.86 +0.3 272,350 + RR42_RS01185 0.86 -1.2 272,350 + RR42_RS01185 0.86 -2.1 272,351 - RR42_RS01185 0.86 -0.8 272,351 - RR42_RS01185 0.86 -1.5 272,351 - RR42_RS01185 0.86 -1.1 272,513 - -0.7 272,604 - +1.0 272,604 - +0.1 272,607 - -0.6 272,607 - +0.0 272,607 - +0.4 272,615 - +0.8 272,615 - -1.0 272,687 - +0.5 272,958 + RR42_RS01190 0.19 +0.0 273,168 + RR42_RS01190 0.41 +0.2 273,168 + RR42_RS01190 0.41 +0.2 273,168 + RR42_RS01190 0.41 +0.4 273,168 + RR42_RS01190 0.41 +0.0 273,169 - RR42_RS01190 0.42 +0.4 273,344 + RR42_RS01190 0.60 +0.2 273,345 - RR42_RS01190 0.60 +0.5 273,345 - RR42_RS01190 0.60 -0.1 273,345 - +0.0 273,345 - RR42_RS01190 0.60 +0.6 273,447 + RR42_RS01190 0.71 +2.0 273,653 + +0.8 273,713 - +0.5 273,720 + -0.2 273,759 - -0.9 273,759 - -0.2 273,763 + +0.1 273,763 + +0.3 273,764 - -0.1 273,764 - +0.6 273,767 + +0.3 273,767 + +0.0 274,200 + RR42_RS01195 0.85 +2.3 274,200 + RR42_RS01195 0.85 +0.6 274,201 - RR42_RS01195 0.85 +0.4 274,224 + -1.9 274,305 - +0.1 274,305 - +0.2 274,305 - -1.9 274,305 - +0.0 274,305 - -0.3
Or see this region's nucleotide sequence