Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00895 and RR42_RS00900 are separated by 119 nucleotides RR42_RS00900 and RR42_RS00905 overlap by 4 nucleotides RR42_RS00905 and RR42_RS00910 overlap by 4 nucleotides
RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947
_RS00895
RR42_RS00900: RR42_RS00900 - 2-hydroxychromene-2-carboxylate isomerase, at 217,067 to 217,768
_RS00900
RR42_RS00905: RR42_RS00905 - haloacid dehalogenase, at 217,765 to 218,454
_RS00905
RR42_RS00910: RR42_RS00910 - hypothetical protein, at 218,451 to 218,876
_RS00910
Position (kb) 217
218 Strain fitness (log2 ratio) -1
0
1
2 at 216.253 kb on - strand, within RR42_RS00895 at 216.479 kb on + strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.480 kb on - strand, within RR42_RS00895 at 216.504 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.600 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.895 kb on - strand at 216.922 kb on + strand at 216.923 kb on - strand at 216.923 kb on - strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.089 kb on - strand at 217.154 kb on + strand, within RR42_RS00900 at 217.250 kb on + strand, within RR42_RS00900 at 217.292 kb on - strand, within RR42_RS00900 at 217.299 kb on - strand, within RR42_RS00900 at 217.730 kb on - strand at 217.899 kb on + strand, within RR42_RS00905 at 217.900 kb on - strand, within RR42_RS00905 at 218.202 kb on + strand, within RR42_RS00905 at 218.203 kb on - strand, within RR42_RS00905 at 218.203 kb on - strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.252 kb on - strand, within RR42_RS00905 at 218.310 kb on + strand, within RR42_RS00905 at 218.425 kb on - strand at 218.592 kb on + strand, within RR42_RS00910 at 218.592 kb on + strand, within RR42_RS00910 at 218.592 kb on + strand, within RR42_RS00910 at 218.639 kb on + strand, within RR42_RS00910 at 218.639 kb on + strand, within RR42_RS00910 at 218.640 kb on - strand, within RR42_RS00910 at 218.640 kb on - strand, within RR42_RS00910 at 218.752 kb on - strand, within RR42_RS00910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 216,253 - RR42_RS00895 0.39 -0.1 216,479 + RR42_RS00895 0.59 +1.0 216,480 - RR42_RS00895 0.59 +0.8 216,480 - RR42_RS00895 0.59 +0.2 216,480 - RR42_RS00895 0.59 +1.4 216,504 + RR42_RS00895 0.61 +0.0 216,600 + RR42_RS00895 0.69 -0.9 216,600 + RR42_RS00895 0.69 +0.0 216,600 + RR42_RS00895 0.69 +0.3 216,774 + RR42_RS00895 0.85 -0.3 216,774 + RR42_RS00895 0.85 +0.4 216,775 - RR42_RS00895 0.85 +0.7 216,775 - RR42_RS00895 0.85 +0.0 216,895 - +0.1 216,922 + -0.7 216,923 - -1.0 216,923 - -0.3 217,088 + -0.3 217,088 + +0.4 217,088 + -0.8 217,089 - +0.1 217,154 + RR42_RS00900 0.12 -0.4 217,250 + RR42_RS00900 0.26 -0.7 217,292 - RR42_RS00900 0.32 +1.0 217,299 - RR42_RS00900 0.33 -0.9 217,730 - +0.0 217,899 + RR42_RS00905 0.19 +0.2 217,900 - RR42_RS00905 0.20 -0.1 218,202 + RR42_RS00905 0.63 -0.6 218,203 - RR42_RS00905 0.63 -0.1 218,203 - RR42_RS00905 0.63 -0.2 218,251 + RR42_RS00905 0.70 +0.1 218,251 + RR42_RS00905 0.70 -0.2 218,251 + RR42_RS00905 0.70 +0.5 218,252 - RR42_RS00905 0.71 -0.3 218,310 + RR42_RS00905 0.79 -1.0 218,425 - -0.6 218,592 + RR42_RS00910 0.33 +0.3 218,592 + RR42_RS00910 0.33 +2.2 218,592 + RR42_RS00910 0.33 -0.4 218,639 + RR42_RS00910 0.44 -1.2 218,639 + RR42_RS00910 0.44 +0.8 218,640 - RR42_RS00910 0.44 -0.4 218,640 - RR42_RS00910 0.44 -1.0 218,752 - RR42_RS00910 0.71 +0.9
Or see this region's nucleotide sequence