Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00825 and RR42_RS00830 are separated by 128 nucleotides RR42_RS00830 and RR42_RS00835 are separated by 91 nucleotides RR42_RS00835 and RR42_RS00840 are separated by 168 nucleotides RR42_RS00840 and RR42_RS00845 overlap by 4 nucleotides
RR42_RS00825: RR42_RS00825 - arginyl-tRNA synthetase, at 201,494 to 203,287
_RS00825
RR42_RS00830: RR42_RS00830 - cell division protein FtsN, at 203,416 to 204,192
_RS00830
RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922
_RS00835
RR42_RS00840: RR42_RS00840 - short-chain dehydrogenase, at 205,091 to 205,903
_RS00840
RR42_RS00845: RR42_RS00845 - metal ABC transporter substrate-binding protein, at 205,900 to 206,736
_RS00845
Position (kb) 204
205 Strain fitness (log2 ratio) -2
-1
0
1 at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.335 kb on + strand at 203.335 kb on + strand at 203.341 kb on + strand at 203.386 kb on + strand at 203.387 kb on - strand at 203.388 kb on + strand at 203.476 kb on - strand at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.540 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.963 kb on - strand, within RR42_RS00830 at 204.082 kb on + strand, within RR42_RS00830 at 204.083 kb on - strand, within RR42_RS00830 at 204.180 kb on + strand at 204.181 kb on - strand at 204.191 kb on - strand at 204.191 kb on - strand at 204.200 kb on + strand at 204.250 kb on + strand at 204.250 kb on + strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.384 kb on - strand, within RR42_RS00835 at 204.450 kb on - strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.788 kb on + strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.830 kb on + strand, within RR42_RS00835 at 204.830 kb on + strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.921 kb on - strand at 204.921 kb on - strand at 205.157 kb on + strand at 205.158 kb on - strand at 205.562 kb on + strand, within RR42_RS00840 at 205.563 kb on - strand, within RR42_RS00840 at 205.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 203,285 + -1.2 203,285 + -0.7 203,285 + -0.4 203,285 + -1.4 203,286 - -0.6 203,286 - -0.0 203,286 - +0.5 203,286 - -2.2 203,335 + +0.4 203,335 + -0.0 203,341 + -1.0 203,386 + -0.5 203,387 - -0.3 203,388 + -0.9 203,476 - -0.8 203,539 + RR42_RS00830 0.16 +1.0 203,539 + RR42_RS00830 0.16 -0.6 203,539 + RR42_RS00830 0.16 +0.2 203,539 + RR42_RS00830 0.16 -0.1 203,540 - RR42_RS00830 0.16 +0.4 203,542 - RR42_RS00830 0.16 -0.1 203,542 - RR42_RS00830 0.16 -0.8 203,542 - RR42_RS00830 0.16 -0.3 203,957 + RR42_RS00830 0.70 -1.0 203,957 + RR42_RS00830 0.70 +0.1 203,957 + RR42_RS00830 0.70 -0.3 203,958 - RR42_RS00830 0.70 -1.6 203,958 - RR42_RS00830 0.70 +0.4 203,963 - RR42_RS00830 0.70 +0.4 204,082 + RR42_RS00830 0.86 -0.0 204,083 - RR42_RS00830 0.86 -1.0 204,180 + +0.4 204,181 - -0.1 204,191 - +0.9 204,191 - +1.3 204,200 + -0.6 204,250 + -0.8 204,250 + -0.7 204,251 - +0.1 204,251 - +0.3 204,251 - +0.4 204,251 - -1.6 204,251 - -1.0 204,251 - -0.6 204,251 - -0.5 204,251 - -0.3 204,384 - RR42_RS00835 0.16 +0.3 204,450 - RR42_RS00835 0.26 -0.0 204,731 + RR42_RS00835 0.70 -1.3 204,731 + RR42_RS00835 0.70 -0.6 204,731 + RR42_RS00835 0.70 +0.0 204,732 - RR42_RS00835 0.70 -0.3 204,732 - RR42_RS00835 0.70 -1.1 204,732 - RR42_RS00835 0.70 +0.6 204,732 - RR42_RS00835 0.70 +0.0 204,732 - RR42_RS00835 0.70 -0.8 204,732 - RR42_RS00835 0.70 -1.2 204,732 - RR42_RS00835 0.70 +0.4 204,732 - RR42_RS00835 0.70 -0.8 204,788 + RR42_RS00835 0.79 -0.1 204,789 - RR42_RS00835 0.79 -0.4 204,789 - RR42_RS00835 0.79 -0.1 204,789 - RR42_RS00835 0.79 -1.3 204,830 + RR42_RS00835 0.85 +0.0 204,830 + RR42_RS00835 0.85 +0.5 204,831 - RR42_RS00835 0.86 +0.0 204,831 - RR42_RS00835 0.86 +1.0 204,831 - RR42_RS00835 0.86 -0.9 204,831 - RR42_RS00835 0.86 +0.3 204,831 - RR42_RS00835 0.86 -0.4 204,831 - RR42_RS00835 0.86 -0.6 204,921 - -0.2 204,921 - -0.6 205,157 + -0.3 205,158 - -1.2 205,562 + RR42_RS00840 0.58 +0.0 205,563 - RR42_RS00840 0.58 -0.8 205,832 + +0.5
Or see this region's nucleotide sequence