Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00740 and RR42_RS00745 are separated by 340 nucleotides RR42_RS00745 and RR42_RS00750 are separated by 58 nucleotides
RR42_RS00740: RR42_RS00740 - ABC transporter substrate-binding protein, at 186,250 to 187,206
_RS00740
RR42_RS00745: RR42_RS00745 - IclR family transcriptional regulator, at 187,547 to 188,383
_RS00745
RR42_RS00750: RR42_RS00750 - hypothetical protein, at 188,442 to 189,422
_RS00750
Position (kb) 187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2 at 186.672 kb on - strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.911 kb on + strand, within RR42_RS00740 at 186.962 kb on + strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 187.202 kb on - strand at 187.286 kb on - strand at 187.358 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.444 kb on + strand at 187.445 kb on - strand at 187.445 kb on - strand at 187.446 kb on + strand at 187.450 kb on - strand at 187.450 kb on - strand at 187.498 kb on - strand at 187.498 kb on - strand at 187.507 kb on + strand at 187.507 kb on + strand at 187.508 kb on - strand at 187.508 kb on - strand at 187.706 kb on + strand, within RR42_RS00745 at 187.764 kb on - strand, within RR42_RS00745 at 188.015 kb on + strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.111 kb on + strand at 188.111 kb on + strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.259 kb on + strand, within RR42_RS00745 at 188.259 kb on + strand, within RR42_RS00745 at 188.260 kb on - strand, within RR42_RS00745 at 188.336 kb on + strand at 188.336 kb on + strand at 188.468 kb on + strand at 188.468 kb on + strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.883 kb on + strand, within RR42_RS00750 at 189.145 kb on - strand, within RR42_RS00750 at 189.145 kb on - strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.239 kb on + strand, within RR42_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 186,672 - RR42_RS00740 0.44 +0.0 186,788 + RR42_RS00740 0.56 -1.1 186,788 + RR42_RS00740 0.56 +0.5 186,788 + RR42_RS00740 0.56 -0.7 186,788 + RR42_RS00740 0.56 +0.2 186,789 - RR42_RS00740 0.56 +0.8 186,789 - RR42_RS00740 0.56 +0.4 186,789 - RR42_RS00740 0.56 +1.5 186,897 - RR42_RS00740 0.68 +1.3 186,897 - RR42_RS00740 0.68 +1.0 186,911 + RR42_RS00740 0.69 +1.3 186,962 + RR42_RS00740 0.74 -1.0 186,963 - RR42_RS00740 0.75 -0.6 186,963 - RR42_RS00740 0.75 -0.4 186,963 - RR42_RS00740 0.75 -0.9 187,202 - -1.3 187,286 - -0.1 187,358 + -1.1 187,378 + +0.6 187,378 + -0.7 187,378 + -0.9 187,378 + +0.3 187,378 + -0.4 187,378 + -0.4 187,378 + +2.6 187,378 + +1.2 187,379 - +1.0 187,379 - -0.5 187,379 - -0.8 187,379 - -0.7 187,380 + -0.6 187,380 + -0.2 187,380 + +0.1 187,380 + +0.6 187,380 + -0.3 187,380 + -0.9 187,380 + -0.2 187,380 + -2.3 187,380 + +0.1 187,380 + +0.2 187,380 + +1.1 187,380 + -1.0 187,381 - -0.6 187,381 - -0.6 187,381 - -1.3 187,381 - +0.3 187,381 - -0.7 187,381 - +0.1 187,381 - -0.4 187,381 - -0.4 187,381 - +1.0 187,444 + +0.8 187,445 - -0.6 187,445 - -0.1 187,446 + -1.1 187,450 - -1.0 187,450 - +0.0 187,498 - +0.4 187,498 - -0.8 187,507 + +1.8 187,507 + -0.1 187,508 - -0.6 187,508 - +0.1 187,706 + RR42_RS00745 0.19 -0.5 187,764 - RR42_RS00745 0.26 -1.0 188,015 + RR42_RS00745 0.56 +0.5 188,016 - RR42_RS00745 0.56 +0.2 188,016 - RR42_RS00745 0.56 -1.4 188,081 - RR42_RS00745 0.64 -2.1 188,081 - RR42_RS00745 0.64 -1.8 188,081 - RR42_RS00745 0.64 +0.1 188,081 - RR42_RS00745 0.64 -0.5 188,111 + +0.4 188,111 + RR42_RS00745 0.67 -0.3 188,112 - RR42_RS00745 0.68 -0.7 188,112 - RR42_RS00745 0.68 -0.9 188,259 + RR42_RS00745 0.85 -1.2 188,259 + RR42_RS00745 0.85 -0.3 188,260 - RR42_RS00745 0.85 -0.7 188,336 + -1.0 188,336 + -0.2 188,468 + -0.7 188,468 + +1.7 188,469 - +0.0 188,469 - -0.1 188,883 + RR42_RS00750 0.45 -0.6 189,145 - RR42_RS00750 0.72 +0.1 189,145 - RR42_RS00750 0.72 +0.4 189,216 + RR42_RS00750 0.79 -0.4 189,216 + RR42_RS00750 0.79 +0.2 189,216 + RR42_RS00750 0.79 -0.6 189,216 + RR42_RS00750 0.79 -0.1 189,216 + RR42_RS00750 0.79 -0.6 189,216 + RR42_RS00750 0.79 +0.2 189,216 + RR42_RS00750 0.79 -0.1 189,216 + RR42_RS00750 0.79 -1.0 189,216 + RR42_RS00750 0.79 +0.5 189,217 - RR42_RS00750 0.79 +0.2 189,217 - RR42_RS00750 0.79 -0.1 189,217 - RR42_RS00750 0.79 -1.0 189,239 + RR42_RS00750 0.81 -0.8
Or see this region's nucleotide sequence