Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00600 and RR42_RS00605 are separated by 106 nucleotides RR42_RS00605 and RR42_RS00610 are separated by 169 nucleotides RR42_RS00610 and RR42_RS00615 are separated by 35 nucleotides
RR42_RS00600: RR42_RS00600 - DeoR family transcriptional regulator, at 157,874 to 158,845
_RS00600
RR42_RS00605: RR42_RS00605 - glutamyl-tRNA amidotransferase, at 158,952 to 160,412
_RS00605
RR42_RS00610: RR42_RS00610 - hypothetical protein, at 160,582 to 161,250
_RS00610
RR42_RS00615: RR42_RS00615 - recombinase XerC, at 161,286 to 162,410
_RS00615
Position (kb) 158
159
160
161 Strain fitness (log2 ratio) -1
0
1 at 158.678 kb on + strand, within RR42_RS00600 at 158.855 kb on + strand at 158.861 kb on + strand at 158.861 kb on + strand at 158.861 kb on + strand at 158.865 kb on + strand at 158.865 kb on + strand at 158.865 kb on + strand at 158.905 kb on + strand at 158.905 kb on + strand at 158.905 kb on + strand at 158.916 kb on + strand at 158.916 kb on + strand at 158.916 kb on + strand at 158.926 kb on + strand at 158.926 kb on + strand at 158.926 kb on + strand at 160.410 kb on + strand at 160.420 kb on - strand at 160.457 kb on + strand at 160.458 kb on - strand at 160.550 kb on - strand at 160.606 kb on + strand at 160.606 kb on + strand at 160.607 kb on - strand at 160.861 kb on + strand at 160.862 kb on - strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 161.011 kb on + strand, within RR42_RS00610 at 161.195 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 158,678 + RR42_RS00600 0.83 -0.0 158,855 + +0.0 158,861 + -1.5 158,861 + +0.4 158,861 + +0.9 158,865 + +0.3 158,865 + +0.1 158,865 + -0.9 158,905 + +0.5 158,905 + +0.6 158,905 + +0.7 158,916 + -0.4 158,916 + -0.0 158,916 + -0.5 158,926 + +1.7 158,926 + +0.0 158,926 + -0.6 160,410 + -0.0 160,420 - -1.0 160,457 + -0.2 160,458 - +0.2 160,550 - +1.1 160,606 + -1.6 160,606 + -0.5 160,607 - +0.4 160,861 + +1.0 160,862 - RR42_RS00610 0.42 -0.0 160,939 + RR42_RS00610 0.53 -0.0 160,939 + RR42_RS00610 0.53 -0.8 160,940 - RR42_RS00610 0.54 -0.4 160,940 - RR42_RS00610 0.54 -1.0 160,940 - RR42_RS00610 0.54 -0.4 160,940 - RR42_RS00610 0.54 +0.1 161,011 + RR42_RS00610 0.64 -0.1 161,195 - +0.7
Or see this region's nucleotide sequence