Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_420 and Pf1N1B4_421 are separated by 14 nucleotides Pf1N1B4_421 and Pf1N1B4_422 are separated by 43 nucleotides
Pf1N1B4_420 - Putative deoxyribonuclease YcfH, at 489,069 to 489,860
_420
Pf1N1B4_421 - Type IV pilus biogenesis protein PilZ, at 489,875 to 490,231
_421
Pf1N1B4_422 - DNA polymerase III delta prime subunit (EC 2.7.7.7), at 490,275 to 491,261
_422
Position (kb)
489
490
491 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 488.880 kb on + strand at 488.880 kb on + strand at 488.880 kb on + strand at 488.930 kb on + strand at 488.956 kb on + strand at 488.957 kb on - strand at 488.962 kb on + strand at 488.962 kb on + strand at 488.962 kb on + strand at 489.071 kb on - strand at 489.146 kb on - strand at 489.189 kb on + strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.193 kb on + strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.277 kb on - strand, within Pf1N1B4_420 at 489.277 kb on - strand, within Pf1N1B4_420 at 489.343 kb on + strand, within Pf1N1B4_420 at 489.343 kb on + strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.346 kb on + strand, within Pf1N1B4_420 at 489.347 kb on - strand, within Pf1N1B4_420 at 489.468 kb on - strand at 489.468 kb on - strand, within Pf1N1B4_420 at 489.556 kb on + strand, within Pf1N1B4_420 at 489.556 kb on + strand, within Pf1N1B4_420 at 489.562 kb on + strand, within Pf1N1B4_420 at 489.563 kb on - strand, within Pf1N1B4_420 at 489.563 kb on - strand, within Pf1N1B4_420 at 489.589 kb on - strand, within Pf1N1B4_420 at 489.835 kb on - strand at 489.835 kb on - strand at 489.835 kb on - strand at 489.852 kb on + strand at 489.860 kb on + strand at 489.861 kb on - strand at 489.861 kb on - strand at 489.861 kb on - strand at 489.876 kb on + strand at 489.876 kb on + strand at 489.876 kb on + strand at 489.876 kb on + strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 490.098 kb on + strand, within Pf1N1B4_421 at 490.099 kb on - strand at 490.099 kb on - strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand at 490.159 kb on - strand, within Pf1N1B4_421 at 490.262 kb on + strand at 490.262 kb on + strand at 490.262 kb on + strand at 490.263 kb on - strand at 490.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 488,880 + -0.2 488,880 + +0.3 488,880 + -1.7 488,930 + +0.1 488,956 + -0.2 488,957 - -0.7 488,962 + -0.0 488,962 + -1.2 488,962 + +2.4 489,071 - -0.2 489,146 - +0.5 489,189 + Pf1N1B4_420 0.15 -0.2 489,190 - Pf1N1B4_420 0.15 +1.6 489,190 - Pf1N1B4_420 0.15 -0.3 489,190 - Pf1N1B4_420 0.15 -0.4 489,190 - Pf1N1B4_420 0.15 +0.4 489,190 - Pf1N1B4_420 0.15 +0.6 489,193 + Pf1N1B4_420 0.16 +0.1 489,194 - Pf1N1B4_420 0.16 +1.3 489,194 - Pf1N1B4_420 0.16 -0.2 489,194 - Pf1N1B4_420 0.16 -2.0 489,194 - Pf1N1B4_420 0.16 +0.3 489,277 - Pf1N1B4_420 0.26 +1.3 489,277 - Pf1N1B4_420 0.26 +1.1 489,343 + Pf1N1B4_420 0.35 -0.4 489,343 + Pf1N1B4_420 0.35 -0.2 489,344 - Pf1N1B4_420 0.35 -0.5 489,344 - Pf1N1B4_420 0.35 -0.3 489,344 - Pf1N1B4_420 0.35 +0.5 489,344 - Pf1N1B4_420 0.35 -1.0 489,344 - Pf1N1B4_420 0.35 -1.2 489,344 - Pf1N1B4_420 0.35 -0.4 489,346 + Pf1N1B4_420 0.35 -1.3 489,347 - Pf1N1B4_420 0.35 +1.4 489,468 - +0.8 489,468 - Pf1N1B4_420 0.50 +0.2 489,556 + Pf1N1B4_420 0.61 +1.4 489,556 + Pf1N1B4_420 0.61 +2.3 489,562 + Pf1N1B4_420 0.62 +0.3 489,563 - Pf1N1B4_420 0.62 +0.6 489,563 - Pf1N1B4_420 0.62 +0.3 489,589 - Pf1N1B4_420 0.66 +0.6 489,835 - +1.6 489,835 - +0.7 489,835 - +0.1 489,852 + +1.6 489,860 + +0.4 489,861 - +0.1 489,861 - +1.8 489,861 - +0.2 489,876 + +0.8 489,876 + -0.2 489,876 + +1.8 489,876 + +0.3 489,877 - -0.7 489,877 - -0.6 489,877 - -1.5 489,877 - +0.1 489,877 - +0.8 489,877 - +1.0 489,877 - -2.2 489,877 - -1.0 489,877 - +1.4 489,877 - +4.0 489,883 + +0.5 489,883 + +0.8 489,883 + -1.7 489,883 + +0.2 489,883 + -0.4 489,884 - -0.9 489,884 - +0.4 489,884 - -0.2 489,884 - +1.3 489,884 - -0.5 489,884 - +0.7 489,884 - +1.4 489,884 - +0.7 490,098 + Pf1N1B4_421 0.62 -0.2 490,099 - -0.2 490,099 - Pf1N1B4_421 0.63 -1.0 490,149 + Pf1N1B4_421 0.77 +0.5 490,149 + Pf1N1B4_421 0.77 -0.8 490,149 + Pf1N1B4_421 0.77 +0.2 490,149 + Pf1N1B4_421 0.77 +1.8 490,149 + Pf1N1B4_421 0.77 -1.7 490,150 - Pf1N1B4_421 0.77 -0.2 490,150 - Pf1N1B4_421 0.77 -1.4 490,150 - Pf1N1B4_421 0.77 -1.7 490,150 - Pf1N1B4_421 0.77 -0.2 490,150 - +0.4 490,159 - Pf1N1B4_421 0.80 -2.0 490,262 + -1.5 490,262 + -1.6 490,262 + -0.2 490,263 - -0.6 490,263 - +0.5
Or see this region's nucleotide sequence