Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3666 and Psyr_3667 are separated by 641 nucleotides Psyr_3667 and Psyr_3668 are separated by 481 nucleotides Psyr_3668 and Psyr_3669 are separated by 239 nucleotides
Psyr_3666: Psyr_3666 - Protein of unknown function DUF195, at 4,352,128 to 4,353,606
_3666
Psyr_3667: Psyr_3667 - conserved hypothetical protein, at 4,354,248 to 4,354,553
_3667
Psyr_3668: Psyr_3668 - conserved domain protein, at 4,355,035 to 4,355,253
_3668
Psyr_3669: Psyr_3669 - Membrane protein involved in aromatic hydrocarbon degradation, at 4,355,493 to 4,356,776
_3669
Position (kb)
4354
4355 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4353.581 kb on - strand at 4353.581 kb on - strand at 4353.628 kb on + strand at 4353.667 kb on - strand at 4353.737 kb on - strand at 4353.766 kb on - strand at 4353.769 kb on - strand at 4353.833 kb on + strand at 4353.924 kb on + strand at 4353.925 kb on - strand at 4353.938 kb on - strand at 4354.039 kb on + strand at 4354.039 kb on + strand at 4354.039 kb on + strand at 4354.039 kb on + strand at 4354.039 kb on + strand at 4354.040 kb on - strand at 4354.040 kb on - strand at 4354.040 kb on - strand at 4354.040 kb on - strand at 4354.040 kb on - strand at 4354.076 kb on + strand at 4354.076 kb on + strand at 4354.139 kb on + strand at 4354.139 kb on + strand at 4354.140 kb on - strand at 4354.140 kb on - strand at 4354.140 kb on - strand at 4354.218 kb on + strand at 4354.219 kb on - strand at 4354.223 kb on + strand at 4354.223 kb on + strand at 4354.223 kb on + strand at 4354.224 kb on - strand at 4354.350 kb on + strand, within Psyr_3667 at 4354.415 kb on + strand, within Psyr_3667 at 4354.415 kb on + strand, within Psyr_3667 at 4354.473 kb on - strand, within Psyr_3667 at 4354.473 kb on - strand, within Psyr_3667 at 4354.523 kb on - strand at 4354.596 kb on - strand at 4354.596 kb on - strand at 4354.598 kb on + strand at 4354.824 kb on - strand at 4354.858 kb on + strand at 4354.858 kb on + strand at 4354.858 kb on + strand at 4354.858 kb on + strand at 4354.858 kb on + strand at 4354.858 kb on + strand at 4354.859 kb on - strand at 4354.859 kb on - strand at 4354.859 kb on - strand at 4354.859 kb on - strand at 4354.859 kb on - strand at 4354.859 kb on - strand at 4354.862 kb on + strand at 4354.862 kb on + strand at 4354.862 kb on + strand at 4354.863 kb on - strand at 4354.863 kb on - strand at 4354.887 kb on + strand at 4354.954 kb on + strand at 4354.954 kb on + strand at 4354.987 kb on + strand at 4355.034 kb on + strand at 4355.034 kb on + strand at 4355.035 kb on - strand at 4355.035 kb on - strand at 4355.041 kb on - strand at 4355.192 kb on - strand, within Psyr_3668 at 4355.310 kb on - strand at 4355.320 kb on + strand at 4355.321 kb on - strand at 4355.321 kb on - strand at 4355.322 kb on + strand at 4355.335 kb on - strand at 4355.377 kb on + strand at 4355.399 kb on + strand at 4355.408 kb on - strand at 4355.408 kb on - strand at 4355.413 kb on + strand at 4355.413 kb on + strand at 4355.433 kb on - strand at 4355.457 kb on + strand at 4355.458 kb on - strand at 4355.488 kb on + strand at 4355.488 kb on + strand at 4355.488 kb on + strand at 4355.488 kb on + strand at 4355.488 kb on + strand at 4355.489 kb on - strand at 4355.489 kb on - strand at 4355.492 kb on - strand at 4355.500 kb on + strand at 4355.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 4,353,581 - +1.3 4,353,581 - -0.3 4,353,628 + -1.2 4,353,667 - +0.0 4,353,737 - -1.7 4,353,766 - -0.1 4,353,769 - +0.4 4,353,833 + +0.0 4,353,924 + -1.2 4,353,925 - +1.7 4,353,938 - -1.4 4,354,039 + +0.7 4,354,039 + -1.1 4,354,039 + +0.5 4,354,039 + -1.5 4,354,039 + -0.2 4,354,040 - -1.1 4,354,040 - +0.5 4,354,040 - +1.4 4,354,040 - +1.2 4,354,040 - +0.7 4,354,076 + -0.4 4,354,076 + -1.1 4,354,139 + +1.8 4,354,139 + +1.1 4,354,140 - -0.5 4,354,140 - -0.4 4,354,140 - +0.2 4,354,218 + +1.9 4,354,219 - -0.0 4,354,223 + +0.9 4,354,223 + -1.3 4,354,223 + -0.1 4,354,224 - +0.9 4,354,350 + Psyr_3667 0.33 -1.2 4,354,415 + Psyr_3667 0.55 -0.1 4,354,415 + Psyr_3667 0.55 -0.9 4,354,473 - Psyr_3667 0.74 -0.1 4,354,473 - Psyr_3667 0.74 +0.0 4,354,523 - -1.2 4,354,596 - -2.7 4,354,596 - -0.7 4,354,598 + +0.9 4,354,824 - -0.1 4,354,858 + +0.9 4,354,858 + +1.4 4,354,858 + -0.4 4,354,858 + -0.7 4,354,858 + -1.0 4,354,858 + -0.7 4,354,859 - -1.4 4,354,859 - -0.9 4,354,859 - +0.2 4,354,859 - +0.7 4,354,859 - -1.1 4,354,859 - -1.1 4,354,862 + -0.1 4,354,862 + +0.8 4,354,862 + +1.1 4,354,863 - -1.1 4,354,863 - -1.4 4,354,887 + -1.7 4,354,954 + +1.1 4,354,954 + +0.7 4,354,987 + -0.7 4,355,034 + -0.4 4,355,034 + -0.1 4,355,035 - +0.3 4,355,035 - -0.4 4,355,041 - -0.6 4,355,192 - Psyr_3668 0.72 -0.4 4,355,310 - -0.1 4,355,320 + +0.5 4,355,321 - -0.7 4,355,321 - -1.3 4,355,322 + +0.5 4,355,335 - -0.1 4,355,377 + -0.6 4,355,399 + -0.4 4,355,408 - +0.8 4,355,408 - -2.0 4,355,413 + +0.2 4,355,413 + -1.5 4,355,433 - -0.4 4,355,457 + +0.4 4,355,458 - +0.1 4,355,488 + -2.1 4,355,488 + -0.9 4,355,488 + +0.2 4,355,488 + +1.9 4,355,488 + +0.4 4,355,489 - +0.3 4,355,489 - -0.9 4,355,492 - +0.3 4,355,500 + +0.7 4,355,501 - -1.8
Or see this region's nucleotide sequence