Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3518 and Psyr_3519 are separated by 8 nucleotides Psyr_3519 and Psyr_3520 are separated by 60 nucleotides Psyr_3520 and Psyr_3521 are separated by 25 nucleotides
Psyr_3518: Psyr_3518 - hypothetical protein, at 4,194,124 to 4,194,519
_3518
Psyr_3519: Psyr_3519 - hypothetical protein, at 4,194,528 to 4,195,148
_3519
Psyr_3520: Psyr_3520 - membrane protein, putative, at 4,195,209 to 4,196,537
_3520
Psyr_3521: Psyr_3521 - transcriptional regulator, LysR family, at 4,196,563 to 4,197,474
_3521
Position (kb) 4195
4196
4197 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4194.235 kb on - strand, within Psyr_3518 at 4194.235 kb on - strand, within Psyr_3518 at 4194.360 kb on + strand, within Psyr_3518 at 4194.361 kb on - strand, within Psyr_3518 at 4194.361 kb on - strand, within Psyr_3518 at 4194.361 kb on - strand, within Psyr_3518 at 4194.363 kb on - strand, within Psyr_3518 at 4194.421 kb on - strand, within Psyr_3518 at 4194.421 kb on - strand, within Psyr_3518 at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand, within Psyr_3519 at 4194.639 kb on - strand, within Psyr_3519 at 4194.842 kb on + strand, within Psyr_3519 at 4194.901 kb on + strand, within Psyr_3519 at 4194.944 kb on + strand, within Psyr_3519 at 4194.945 kb on - strand, within Psyr_3519 at 4195.068 kb on - strand, within Psyr_3519 at 4195.168 kb on + strand at 4195.168 kb on + strand at 4195.168 kb on + strand at 4195.168 kb on + strand at 4195.272 kb on + strand at 4195.272 kb on + strand at 4195.272 kb on + strand at 4195.273 kb on - strand at 4195.273 kb on - strand at 4195.273 kb on - strand at 4195.273 kb on - strand at 4195.273 kb on - strand at 4195.273 kb on - strand at 4195.273 kb on - strand at 4195.699 kb on - strand, within Psyr_3520 at 4195.740 kb on + strand, within Psyr_3520 at 4195.971 kb on + strand, within Psyr_3520 at 4195.971 kb on + strand, within Psyr_3520 at 4195.971 kb on + strand, within Psyr_3520 at 4195.971 kb on + strand, within Psyr_3520 at 4195.971 kb on + strand, within Psyr_3520 at 4195.972 kb on - strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.172 kb on + strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.173 kb on - strand, within Psyr_3520 at 4196.495 kb on + strand at 4196.648 kb on + strand at 4196.648 kb on + strand at 4196.648 kb on + strand at 4196.840 kb on + strand, within Psyr_3521 at 4196.841 kb on - strand, within Psyr_3521 at 4196.864 kb on + strand, within Psyr_3521 at 4196.865 kb on - strand, within Psyr_3521 at 4196.865 kb on - strand, within Psyr_3521 at 4196.865 kb on - strand, within Psyr_3521 at 4197.206 kb on + strand, within Psyr_3521 at 4197.207 kb on - strand, within Psyr_3521 at 4197.500 kb on + strand at 4197.500 kb on + strand at 4197.500 kb on + strand at 4197.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 4,194,235 - Psyr_3518 0.28 -0.1 4,194,235 - Psyr_3518 0.28 -0.2 4,194,360 + Psyr_3518 0.60 -0.3 4,194,361 - Psyr_3518 0.60 -0.1 4,194,361 - Psyr_3518 0.60 +1.5 4,194,361 - Psyr_3518 0.60 -0.4 4,194,363 - Psyr_3518 0.60 +1.3 4,194,421 - Psyr_3518 0.75 +0.6 4,194,421 - Psyr_3518 0.75 +0.1 4,194,639 - Psyr_3519 0.18 +0.9 4,194,639 - Psyr_3519 0.18 +0.6 4,194,639 - Psyr_3519 0.18 -1.0 4,194,639 - Psyr_3519 0.18 +0.3 4,194,639 - +2.5 4,194,639 - Psyr_3519 0.18 -1.0 4,194,639 - Psyr_3519 0.18 -0.4 4,194,639 - Psyr_3519 0.18 +1.7 4,194,639 - Psyr_3519 0.18 -0.7 4,194,842 + Psyr_3519 0.51 -2.0 4,194,901 + Psyr_3519 0.60 +0.7 4,194,944 + Psyr_3519 0.67 -2.4 4,194,945 - Psyr_3519 0.67 +0.2 4,195,068 - Psyr_3519 0.87 -1.0 4,195,168 + +1.1 4,195,168 + +1.1 4,195,168 + +0.5 4,195,168 + -1.1 4,195,272 + -0.5 4,195,272 + -0.7 4,195,272 + +0.9 4,195,273 - +0.5 4,195,273 - +0.4 4,195,273 - -0.7 4,195,273 - -1.1 4,195,273 - +0.7 4,195,273 - +1.1 4,195,273 - +0.3 4,195,699 - Psyr_3520 0.37 +0.3 4,195,740 + Psyr_3520 0.40 +0.8 4,195,971 + Psyr_3520 0.57 +0.4 4,195,971 + Psyr_3520 0.57 +0.6 4,195,971 + Psyr_3520 0.57 -1.4 4,195,971 + Psyr_3520 0.57 +0.4 4,195,971 + Psyr_3520 0.57 +0.2 4,195,972 - Psyr_3520 0.57 +0.4 4,196,172 + Psyr_3520 0.72 +0.9 4,196,172 + Psyr_3520 0.72 -0.3 4,196,172 + Psyr_3520 0.72 +0.9 4,196,172 + Psyr_3520 0.72 +0.3 4,196,172 + Psyr_3520 0.72 +0.9 4,196,172 + Psyr_3520 0.72 -0.0 4,196,172 + Psyr_3520 0.72 +0.3 4,196,172 + Psyr_3520 0.72 +0.9 4,196,172 + Psyr_3520 0.72 +0.0 4,196,172 + Psyr_3520 0.72 +2.2 4,196,172 + Psyr_3520 0.72 +0.6 4,196,173 - Psyr_3520 0.73 +0.6 4,196,173 - Psyr_3520 0.73 +0.1 4,196,173 - Psyr_3520 0.73 -0.9 4,196,173 - Psyr_3520 0.73 -0.4 4,196,173 - Psyr_3520 0.73 +0.4 4,196,173 - Psyr_3520 0.73 -0.7 4,196,173 - Psyr_3520 0.73 -0.3 4,196,173 - Psyr_3520 0.73 -0.9 4,196,173 - Psyr_3520 0.73 +0.7 4,196,173 - Psyr_3520 0.73 -0.3 4,196,173 - Psyr_3520 0.73 +2.2 4,196,495 + +0.3 4,196,648 + +0.4 4,196,648 + -1.4 4,196,648 + +1.5 4,196,840 + Psyr_3521 0.30 -1.0 4,196,841 - Psyr_3521 0.30 +0.4 4,196,864 + Psyr_3521 0.33 -0.1 4,196,865 - Psyr_3521 0.33 -1.0 4,196,865 - Psyr_3521 0.33 +0.4 4,196,865 - Psyr_3521 0.33 -2.6 4,197,206 + Psyr_3521 0.71 +0.3 4,197,207 - Psyr_3521 0.71 -1.1 4,197,500 + +1.6 4,197,500 + -1.3 4,197,500 + +1.1 4,197,501 - -2.1
Or see this region's nucleotide sequence