Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2088 and Psyr_2089 are separated by 30 nucleotides Psyr_2089 and Psyr_2090 are separated by 221 nucleotides
Psyr_2088: Psyr_2088 - 2-methylaconitate cis-trans isomerase, at 2,435,367 to 2,436,557
_2088
Psyr_2089: Psyr_2089 - Protein of unknown function DUF299, at 2,436,588 to 2,437,406
_2089
Psyr_2090: Psyr_2090 - phosphoenolpyruvate synthase, at 2,437,628 to 2,440,003
_2090
Position (kb)
2436
2437
2438 Strain fitness (log2 ratio)
-2
-1
0
1 at 2435.622 kb on + strand, within Psyr_2088 at 2435.623 kb on - strand, within Psyr_2088 at 2436.228 kb on + strand, within Psyr_2088 at 2436.589 kb on + strand at 2436.590 kb on - strand at 2436.881 kb on - strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.916 kb on + strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.917 kb on - strand, within Psyr_2089 at 2436.979 kb on + strand, within Psyr_2089 at 2436.980 kb on - strand, within Psyr_2089 at 2436.980 kb on - strand, within Psyr_2089 at 2436.982 kb on + strand, within Psyr_2089 at 2436.983 kb on - strand, within Psyr_2089 at 2437.045 kb on + strand, within Psyr_2089 at 2437.046 kb on - strand, within Psyr_2089 at 2437.046 kb on - strand, within Psyr_2089 at 2437.055 kb on + strand, within Psyr_2089 at 2437.055 kb on + strand, within Psyr_2089 at 2437.055 kb on + strand, within Psyr_2089 at 2437.087 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.103 kb on + strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.104 kb on - strand, within Psyr_2089 at 2437.257 kb on + strand, within Psyr_2089 at 2437.291 kb on + strand, within Psyr_2089 at 2437.291 kb on + strand, within Psyr_2089 at 2437.292 kb on - strand, within Psyr_2089 at 2437.292 kb on - strand, within Psyr_2089 at 2437.316 kb on + strand, within Psyr_2089 at 2437.316 kb on + strand, within Psyr_2089 at 2437.449 kb on + strand at 2437.449 kb on + strand at 2437.449 kb on + strand at 2437.449 kb on + strand at 2437.450 kb on - strand at 2437.450 kb on - strand at 2437.450 kb on - strand at 2437.450 kb on - strand at 2437.536 kb on - strand at 2437.536 kb on - strand at 2437.613 kb on - strand at 2437.633 kb on - strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.637 kb on + strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.638 kb on - strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.641 kb on + strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.642 kb on - strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.674 kb on + strand at 2437.675 kb on - strand at 2437.675 kb on - strand at 2437.940 kb on - strand, within Psyr_2090 at 2438.337 kb on - strand, within Psyr_2090 at 2438.337 kb on - strand, within Psyr_2090
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 2,435,622 + Psyr_2088 0.21 -1.7 2,435,623 - Psyr_2088 0.21 +0.8 2,436,228 + Psyr_2088 0.72 +1.6 2,436,589 + -0.6 2,436,590 - -0.2 2,436,881 - Psyr_2089 0.36 +1.5 2,436,916 + Psyr_2089 0.40 +0.1 2,436,916 + Psyr_2089 0.40 +0.3 2,436,916 + Psyr_2089 0.40 +0.9 2,436,916 + Psyr_2089 0.40 -0.5 2,436,916 + Psyr_2089 0.40 -0.6 2,436,916 + Psyr_2089 0.40 +1.1 2,436,917 - Psyr_2089 0.40 -0.2 2,436,917 - Psyr_2089 0.40 -1.4 2,436,917 - Psyr_2089 0.40 +0.5 2,436,917 - Psyr_2089 0.40 -0.8 2,436,917 - Psyr_2089 0.40 -1.6 2,436,917 - Psyr_2089 0.40 -0.7 2,436,979 + Psyr_2089 0.48 -1.5 2,436,980 - Psyr_2089 0.48 -2.1 2,436,980 - Psyr_2089 0.48 +0.5 2,436,982 + Psyr_2089 0.48 -0.9 2,436,983 - Psyr_2089 0.48 -1.3 2,437,045 + Psyr_2089 0.56 -0.7 2,437,046 - Psyr_2089 0.56 +0.5 2,437,046 - Psyr_2089 0.56 -1.7 2,437,055 + Psyr_2089 0.57 -0.5 2,437,055 + Psyr_2089 0.57 +1.3 2,437,055 + Psyr_2089 0.57 -0.8 2,437,087 + Psyr_2089 0.61 -0.3 2,437,103 + Psyr_2089 0.63 -0.5 2,437,103 + Psyr_2089 0.63 -0.5 2,437,103 + Psyr_2089 0.63 -1.1 2,437,103 + Psyr_2089 0.63 +0.5 2,437,103 + Psyr_2089 0.63 -0.3 2,437,104 - Psyr_2089 0.63 -0.2 2,437,104 - Psyr_2089 0.63 -0.9 2,437,104 - Psyr_2089 0.63 +0.2 2,437,104 - Psyr_2089 0.63 +1.1 2,437,257 + Psyr_2089 0.82 -1.3 2,437,291 + Psyr_2089 0.86 -0.4 2,437,291 + Psyr_2089 0.86 +0.1 2,437,292 - Psyr_2089 0.86 -1.1 2,437,292 - Psyr_2089 0.86 -0.9 2,437,316 + Psyr_2089 0.89 -0.8 2,437,316 + Psyr_2089 0.89 -1.3 2,437,449 + +0.9 2,437,449 + -1.1 2,437,449 + -0.3 2,437,449 + +0.9 2,437,450 - -1.3 2,437,450 - +0.1 2,437,450 - -0.2 2,437,450 - -1.5 2,437,536 - +0.3 2,437,536 - -0.0 2,437,613 - -0.4 2,437,633 - +0.8 2,437,637 + +1.6 2,437,637 + +1.1 2,437,637 + +1.5 2,437,637 + +0.5 2,437,637 + +1.1 2,437,637 + +0.3 2,437,638 - -0.9 2,437,638 - -0.4 2,437,638 - -0.6 2,437,638 - -0.8 2,437,638 - +0.9 2,437,641 + -0.3 2,437,641 + -1.5 2,437,641 + +0.6 2,437,641 + -0.8 2,437,641 + +0.6 2,437,642 - -0.4 2,437,642 - -0.5 2,437,642 - +0.7 2,437,642 - -0.8 2,437,642 - -0.4 2,437,642 - +0.3 2,437,674 + -0.7 2,437,674 + -0.5 2,437,674 + -0.9 2,437,674 + +1.5 2,437,674 + +1.1 2,437,675 - -0.6 2,437,675 - +1.1 2,437,940 - Psyr_2090 0.13 -0.1 2,438,337 - Psyr_2090 0.30 +1.1 2,438,337 - Psyr_2090 0.30 +0.3
Or see this region's nucleotide sequence