Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1979 and Psyr_1980 are separated by 129 nucleotides Psyr_1980 and Psyr_1981 are separated by 67 nucleotides Psyr_1981 and Psyr_1982 are separated by 63 nucleotides
Psyr_1979: Psyr_1979 - tRNA-U20a,U20b-dihydrouridine synthase, at 2,297,844 to 2,298,809
_1979
Psyr_1980: Psyr_1980 - Heat shock protein Hsp20, at 2,298,939 to 2,299,382
_1980
Psyr_1981: Psyr_1981 - PAS:GGDEF, at 2,299,450 to 2,301,642
_1981
Psyr_1982: Psyr_1982 - transcriptional regulator, LysR family, at 2,301,706 to 2,302,578
_1982
Position (kb)
2299
2300
2301
2302 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.732 kb on - strand at 2298.732 kb on - strand at 2298.769 kb on + strand at 2298.770 kb on - strand at 2298.770 kb on - strand at 2298.870 kb on + strand at 2298.871 kb on - strand at 2298.908 kb on - strand at 2298.944 kb on + strand at 2298.945 kb on - strand at 2299.053 kb on + strand, within Psyr_1980 at 2299.053 kb on + strand, within Psyr_1980 at 2299.054 kb on - strand, within Psyr_1980 at 2299.200 kb on + strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.259 kb on + strand, within Psyr_1980 at 2299.413 kb on + strand at 2299.414 kb on - strand at 2299.538 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.553 kb on + strand at 2299.554 kb on - strand at 2299.554 kb on - strand at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.851 kb on + strand, within Psyr_1981 at 2299.852 kb on - strand, within Psyr_1981 at 2299.960 kb on - strand, within Psyr_1981 at 2300.043 kb on + strand, within Psyr_1981 at 2300.043 kb on + strand, within Psyr_1981 at 2300.044 kb on - strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.117 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.527 kb on + strand, within Psyr_1981 at 2300.884 kb on + strand, within Psyr_1981 at 2300.884 kb on + strand, within Psyr_1981 at 2300.886 kb on + strand, within Psyr_1981 at 2300.887 kb on - strand, within Psyr_1981 at 2300.887 kb on - strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.086 kb on + strand, within Psyr_1981 at 2301.305 kb on + strand, within Psyr_1981 at 2301.350 kb on + strand, within Psyr_1981 at 2301.351 kb on - strand, within Psyr_1981 at 2301.351 kb on - strand, within Psyr_1981 at 2301.704 kb on - strand at 2301.782 kb on + strand at 2301.783 kb on - strand at 2301.783 kb on - strand at 2301.787 kb on + strand at 2301.788 kb on - strand at 2301.788 kb on - strand at 2301.788 kb on - strand at 2301.805 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.907 kb on - strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.979 kb on - strand, within Psyr_1982 at 2302.021 kb on - strand, within Psyr_1982 at 2302.140 kb on + strand, within Psyr_1982 at 2302.140 kb on + strand, within Psyr_1982 at 2302.141 kb on - strand, within Psyr_1982 at 2302.252 kb on + strand, within Psyr_1982 at 2302.252 kb on + strand, within Psyr_1982 at 2302.373 kb on + strand, within Psyr_1982 at 2302.374 kb on - strand, within Psyr_1982 at 2302.638 kb on + strand at 2302.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 2,298,731 + +1.0 2,298,731 + +0.6 2,298,731 + -1.3 2,298,732 - -1.4 2,298,732 - -0.6 2,298,769 + +0.3 2,298,770 - +1.9 2,298,770 - -0.4 2,298,870 + +1.7 2,298,871 - -0.2 2,298,908 - +0.7 2,298,944 + -1.0 2,298,945 - -0.4 2,299,053 + Psyr_1980 0.26 -0.3 2,299,053 + Psyr_1980 0.26 +1.1 2,299,054 - Psyr_1980 0.26 +0.0 2,299,200 + Psyr_1980 0.59 +1.3 2,299,201 - Psyr_1980 0.59 +0.5 2,299,201 - Psyr_1980 0.59 +0.6 2,299,201 - Psyr_1980 0.59 +0.4 2,299,259 + Psyr_1980 0.72 -1.2 2,299,413 + -0.2 2,299,414 - -1.8 2,299,538 + -0.2 2,299,547 + +0.1 2,299,547 + -0.6 2,299,547 - -0.6 2,299,548 - +0.3 2,299,548 - +0.3 2,299,548 - -1.4 2,299,553 + -1.9 2,299,554 - -0.6 2,299,554 - +1.1 2,299,742 + Psyr_1981 0.13 -0.3 2,299,742 + Psyr_1981 0.13 +1.8 2,299,742 + Psyr_1981 0.13 -1.0 2,299,742 + Psyr_1981 0.13 -2.0 2,299,742 + Psyr_1981 0.13 +0.1 2,299,743 - Psyr_1981 0.13 -1.6 2,299,743 - Psyr_1981 0.13 +0.7 2,299,851 + Psyr_1981 0.18 -0.9 2,299,852 - Psyr_1981 0.18 -1.2 2,299,960 - Psyr_1981 0.23 -1.2 2,300,043 + Psyr_1981 0.27 -0.1 2,300,043 + Psyr_1981 0.27 -1.0 2,300,044 - Psyr_1981 0.27 -0.9 2,300,083 + Psyr_1981 0.29 -1.3 2,300,083 + Psyr_1981 0.29 -1.7 2,300,083 + Psyr_1981 0.29 -0.7 2,300,084 - Psyr_1981 0.29 +0.1 2,300,084 - Psyr_1981 0.29 -0.7 2,300,084 - Psyr_1981 0.29 -0.2 2,300,117 + Psyr_1981 0.30 +0.1 2,300,162 + Psyr_1981 0.32 -0.6 2,300,162 + Psyr_1981 0.32 -1.2 2,300,162 + Psyr_1981 0.32 -1.7 2,300,162 + Psyr_1981 0.32 -0.9 2,300,162 + Psyr_1981 0.32 -0.5 2,300,163 - Psyr_1981 0.33 +1.1 2,300,163 - Psyr_1981 0.33 -2.0 2,300,163 - Psyr_1981 0.33 -2.0 2,300,163 - Psyr_1981 0.33 -0.1 2,300,163 - Psyr_1981 0.33 -0.2 2,300,163 - Psyr_1981 0.33 -0.7 2,300,527 + Psyr_1981 0.49 +1.7 2,300,884 + Psyr_1981 0.65 -0.5 2,300,884 + Psyr_1981 0.65 -1.5 2,300,886 + Psyr_1981 0.65 -0.2 2,300,887 - Psyr_1981 0.66 -0.5 2,300,887 - Psyr_1981 0.66 -1.0 2,301,021 + Psyr_1981 0.72 -0.0 2,301,021 + Psyr_1981 0.72 -0.4 2,301,021 + Psyr_1981 0.72 -0.3 2,301,086 + Psyr_1981 0.75 -1.3 2,301,305 + Psyr_1981 0.85 -1.2 2,301,350 + Psyr_1981 0.87 -1.1 2,301,351 - Psyr_1981 0.87 -0.2 2,301,351 - Psyr_1981 0.87 -2.0 2,301,704 - -0.8 2,301,782 + -1.2 2,301,783 - -0.5 2,301,783 - +2.1 2,301,787 + -0.9 2,301,788 - -0.7 2,301,788 - -0.3 2,301,788 - -0.4 2,301,805 + Psyr_1982 0.11 -2.6 2,301,906 + Psyr_1982 0.23 -2.2 2,301,906 + Psyr_1982 0.23 -1.5 2,301,906 + Psyr_1982 0.23 +0.3 2,301,907 - Psyr_1982 0.23 -0.7 2,301,908 + Psyr_1982 0.23 -1.7 2,301,908 + Psyr_1982 0.23 +0.5 2,301,908 + Psyr_1982 0.23 +1.9 2,301,908 + Psyr_1982 0.23 -0.3 2,301,908 + Psyr_1982 0.23 -1.3 2,301,908 + Psyr_1982 0.23 +1.1 2,301,908 + Psyr_1982 0.23 -0.1 2,301,908 + Psyr_1982 0.23 +0.3 2,301,908 + Psyr_1982 0.23 -0.3 2,301,908 + Psyr_1982 0.23 -0.2 2,301,908 + Psyr_1982 0.23 +0.6 2,301,909 - Psyr_1982 0.23 +0.0 2,301,909 - Psyr_1982 0.23 +0.8 2,301,909 - Psyr_1982 0.23 -0.1 2,301,909 - Psyr_1982 0.23 -2.4 2,301,909 - Psyr_1982 0.23 -0.9 2,301,909 - Psyr_1982 0.23 -0.9 2,301,909 - Psyr_1982 0.23 +0.1 2,301,909 - Psyr_1982 0.23 -0.2 2,301,909 - Psyr_1982 0.23 -0.1 2,301,909 - Psyr_1982 0.23 -1.3 2,301,909 - Psyr_1982 0.23 -0.2 2,301,909 - Psyr_1982 0.23 +2.1 2,301,909 - Psyr_1982 0.23 -2.0 2,301,909 - Psyr_1982 0.23 +0.6 2,301,909 - Psyr_1982 0.23 -0.2 2,301,909 - Psyr_1982 0.23 -0.3 2,301,979 - Psyr_1982 0.31 +0.1 2,302,021 - Psyr_1982 0.36 +0.9 2,302,140 + Psyr_1982 0.50 +0.1 2,302,140 + Psyr_1982 0.50 +0.1 2,302,141 - Psyr_1982 0.50 +0.7 2,302,252 + Psyr_1982 0.63 -1.3 2,302,252 + Psyr_1982 0.63 +2.5 2,302,373 + Psyr_1982 0.76 -2.6 2,302,374 - Psyr_1982 0.77 -1.4 2,302,638 + +0.3 2,302,639 - +0.4
Or see this region's nucleotide sequence