Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1020 and Psyr_1021 are separated by 21 nucleotides Psyr_1021 and Psyr_1022 are separated by 234 nucleotides Psyr_1022 and Psyr_1023 are separated by 151 nucleotides Psyr_1023 and Psyr_1024 are separated by 206 nucleotides
Psyr_1020: Psyr_1020 - GTP cyclohydrolase I, at 1,151,982 to 1,152,545
_1020
Psyr_1021: Psyr_1021 - Short-chain dehydrogenase/reductase SDR, at 1,152,567 to 1,153,277
_1021
Psyr_1022: Psyr_1022 - conserved hypothetical protein, at 1,153,512 to 1,154,078
_1022
Psyr_1023: Psyr_1023 - conserved hypothetical protein, at 1,154,230 to 1,154,835
_1023
Psyr_1024: Psyr_1024 - Flavodoxin/nitric oxide synthase, at 1,155,042 to 1,155,494
_1024
Position (kb) 1153
1154
1155 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1152.568 kb on + strand at 1152.568 kb on + strand at 1152.569 kb on - strand at 1152.569 kb on - strand at 1152.569 kb on - strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.634 kb on + strand at 1152.649 kb on + strand, within Psyr_1021 at 1152.673 kb on - strand, within Psyr_1021 at 1152.710 kb on - strand, within Psyr_1021 at 1152.753 kb on + strand, within Psyr_1021 at 1152.753 kb on + strand, within Psyr_1021 at 1152.754 kb on - strand, within Psyr_1021 at 1152.754 kb on - strand, within Psyr_1021 at 1152.881 kb on + strand, within Psyr_1021 at 1152.881 kb on + strand, within Psyr_1021 at 1153.227 kb on + strand at 1153.228 kb on - strand at 1153.228 kb on - strand at 1153.228 kb on - strand at 1153.256 kb on + strand at 1153.290 kb on + strand at 1153.319 kb on + strand at 1153.320 kb on - strand at 1153.356 kb on - strand at 1153.375 kb on + strand at 1153.505 kb on + strand at 1153.506 kb on - strand at 1153.513 kb on + strand at 1153.606 kb on + strand, within Psyr_1022 at 1153.607 kb on - strand, within Psyr_1022 at 1153.607 kb on - strand, within Psyr_1022 at 1153.607 kb on - strand, within Psyr_1022 at 1154.072 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.083 kb on + strand at 1154.083 kb on + strand at 1154.083 kb on + strand at 1154.084 kb on - strand at 1154.084 kb on - strand at 1154.084 kb on - strand at 1154.183 kb on - strand at 1154.231 kb on + strand at 1154.232 kb on - strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.277 kb on - strand at 1154.277 kb on - strand at 1154.277 kb on - strand at 1154.277 kb on - strand at 1154.325 kb on - strand, within Psyr_1023 at 1154.325 kb on - strand, within Psyr_1023 at 1154.376 kb on + strand, within Psyr_1023 at 1154.574 kb on + strand, within Psyr_1023 at 1154.574 kb on + strand, within Psyr_1023 at 1154.749 kb on - strand, within Psyr_1023 at 1154.749 kb on - strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.998 kb on + strand at 1154.998 kb on + strand at 1154.998 kb on + strand at 1155.005 kb on + strand at 1155.005 kb on + strand at 1155.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 1,152,568 + -1.1 1,152,568 + +0.4 1,152,569 - -2.2 1,152,569 - +0.7 1,152,569 - +0.6 1,152,616 + -0.2 1,152,616 + -0.9 1,152,616 + +0.5 1,152,616 + -0.8 1,152,616 + +0.4 1,152,616 + -0.1 1,152,616 + -1.1 1,152,616 + -0.4 1,152,616 + +0.3 1,152,616 + +0.6 1,152,616 + -1.8 1,152,616 + -0.1 1,152,616 + -1.4 1,152,617 - +0.1 1,152,617 - +0.4 1,152,617 - +0.5 1,152,617 - +0.4 1,152,617 - +0.3 1,152,617 - -1.1 1,152,617 - +0.4 1,152,617 - +0.9 1,152,617 - -0.7 1,152,617 - +1.2 1,152,617 - -1.1 1,152,617 - +0.6 1,152,617 - +1.1 1,152,617 - +0.3 1,152,617 - +0.4 1,152,617 - +0.2 1,152,617 - -0.4 1,152,617 - +0.3 1,152,617 - -0.8 1,152,634 + +0.3 1,152,649 + Psyr_1021 0.12 -0.1 1,152,673 - Psyr_1021 0.15 +0.1 1,152,710 - Psyr_1021 0.20 +0.9 1,152,753 + Psyr_1021 0.26 -0.2 1,152,753 + Psyr_1021 0.26 +0.9 1,152,754 - Psyr_1021 0.26 +0.4 1,152,754 - Psyr_1021 0.26 +0.2 1,152,881 + Psyr_1021 0.44 -0.2 1,152,881 + Psyr_1021 0.44 -1.7 1,153,227 + -0.7 1,153,228 - +0.6 1,153,228 - -1.5 1,153,228 - -0.3 1,153,256 + -0.9 1,153,290 + -0.2 1,153,319 + -1.0 1,153,320 - -1.4 1,153,356 - +0.5 1,153,375 + +0.4 1,153,505 + -0.6 1,153,506 - +0.4 1,153,513 + +1.7 1,153,606 + Psyr_1022 0.17 -0.7 1,153,607 - Psyr_1022 0.17 +0.3 1,153,607 - Psyr_1022 0.17 -2.0 1,153,607 - Psyr_1022 0.17 -1.7 1,154,072 + -1.1 1,154,081 + +0.2 1,154,081 + +0.4 1,154,081 + +1.5 1,154,081 + +0.1 1,154,081 + -0.1 1,154,081 + +0.6 1,154,081 + -0.8 1,154,081 + +2.2 1,154,081 + +0.4 1,154,081 + +0.0 1,154,081 + +0.3 1,154,081 + +0.2 1,154,081 + +0.1 1,154,081 + -0.7 1,154,081 + +2.2 1,154,081 + +0.6 1,154,081 + -0.1 1,154,081 + +0.2 1,154,081 + +0.1 1,154,081 + -0.2 1,154,081 + -0.2 1,154,081 + -0.3 1,154,081 + -0.9 1,154,081 + -0.3 1,154,081 + -0.0 1,154,081 + +0.3 1,154,081 + +0.3 1,154,081 + -0.1 1,154,081 + +2.5 1,154,081 + +0.2 1,154,081 + +0.1 1,154,081 + +0.3 1,154,081 + +0.5 1,154,081 + +1.4 1,154,081 + -0.7 1,154,081 + -0.7 1,154,081 + +0.6 1,154,081 + -2.1 1,154,081 + -0.7 1,154,081 + -1.2 1,154,081 + +1.1 1,154,081 + +1.7 1,154,081 + +1.2 1,154,081 + +0.0 1,154,081 + -1.9 1,154,081 + +0.0 1,154,082 - +1.5 1,154,082 - +0.1 1,154,082 - +0.0 1,154,082 - -0.7 1,154,082 - +0.2 1,154,082 - -0.1 1,154,082 - +0.3 1,154,082 - -0.3 1,154,082 - +0.2 1,154,082 - -1.0 1,154,082 - +0.9 1,154,082 - +0.7 1,154,082 - +0.2 1,154,082 - +1.7 1,154,082 - -0.1 1,154,082 - -0.1 1,154,082 - -0.1 1,154,082 - -0.5 1,154,082 - +0.1 1,154,082 - -0.8 1,154,082 - -0.3 1,154,082 - +0.2 1,154,082 - +0.6 1,154,082 - +0.7 1,154,082 - +1.4 1,154,082 - -0.1 1,154,082 - -0.7 1,154,082 - +0.8 1,154,082 - +1.6 1,154,082 - -0.1 1,154,082 - +0.2 1,154,082 - +0.5 1,154,082 - +0.2 1,154,082 - -1.2 1,154,082 - +0.8 1,154,082 - -0.7 1,154,082 - -0.5 1,154,082 - -0.9 1,154,082 - -0.3 1,154,082 - -1.1 1,154,083 + -0.4 1,154,083 + +0.5 1,154,083 + -0.0 1,154,084 - +0.2 1,154,084 - +0.7 1,154,084 - +0.4 1,154,183 - -0.3 1,154,231 + +2.0 1,154,232 - -1.7 1,154,276 + +0.4 1,154,276 + -0.1 1,154,276 + -2.0 1,154,276 + +0.1 1,154,276 + -2.3 1,154,276 + -1.4 1,154,276 + -1.0 1,154,277 - -0.7 1,154,277 - -0.1 1,154,277 - +0.1 1,154,277 - +0.1 1,154,325 - Psyr_1023 0.16 -1.9 1,154,325 - Psyr_1023 0.16 -0.3 1,154,376 + Psyr_1023 0.24 -0.8 1,154,574 + Psyr_1023 0.57 +0.2 1,154,574 + Psyr_1023 0.57 -1.6 1,154,749 - Psyr_1023 0.86 +0.9 1,154,749 - Psyr_1023 0.86 +0.2 1,154,758 + Psyr_1023 0.87 +0.2 1,154,758 + Psyr_1023 0.87 +0.6 1,154,758 + Psyr_1023 0.87 -0.5 1,154,758 + Psyr_1023 0.87 +0.1 1,154,759 - Psyr_1023 0.87 -0.0 1,154,759 - Psyr_1023 0.87 -1.9 1,154,759 - Psyr_1023 0.87 -0.3 1,154,759 - Psyr_1023 0.87 +0.7 1,154,759 - Psyr_1023 0.87 -1.8 1,154,759 - Psyr_1023 0.87 +0.3 1,154,759 - Psyr_1023 0.87 -1.2 1,154,759 - Psyr_1023 0.87 -0.1 1,154,759 - Psyr_1023 0.87 -0.4 1,154,998 + +0.1 1,154,998 + -0.4 1,154,998 + +1.2 1,155,005 + -0.8 1,155,005 + -0.1 1,155,006 - +0.2
Or see this region's nucleotide sequence