Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0509 and Psyr_0510 are separated by 32 nucleotides Psyr_0510 and Psyr_0511 are separated by 26 nucleotides Psyr_0511 and Psyr_0512 are separated by 7 nucleotides Psyr_0512 and Psyr_0513 are separated by 226 nucleotides
Psyr_0509: Psyr_0509 - PAS:GGDEF, at 564,626 to 566,785
_0509
Psyr_0510: Psyr_0510 - conserved hypothetical protein, at 566,818 to 567,342
_0510
Psyr_0511: Psyr_0511 - conserved hypothetical protein, at 567,369 to 567,665
_0511
Psyr_0512: Psyr_0512 - GCN5-related N-acetyltransferase, at 567,673 to 568,131
_0512
Psyr_0513: Psyr_0513 - amino acid ABC transporter substrate-binding protein, PAAT family, at 568,358 to 569,113
_0513
Position (kb)
567
568 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 566.545 kb on + strand, within Psyr_0509 at 566.584 kb on + strand at 566.585 kb on - strand at 566.783 kb on + strand at 566.783 kb on + strand at 566.783 kb on + strand at 566.784 kb on - strand at 566.784 kb on - strand at 566.784 kb on - strand at 566.784 kb on - strand at 566.849 kb on - strand at 566.921 kb on - strand, within Psyr_0510 at 566.974 kb on + strand, within Psyr_0510 at 567.239 kb on - strand, within Psyr_0510 at 567.239 kb on - strand, within Psyr_0510 at 567.239 kb on - strand, within Psyr_0510 at 567.239 kb on - strand, within Psyr_0510 at 567.299 kb on - strand at 567.308 kb on - strand at 567.399 kb on + strand, within Psyr_0511 at 567.411 kb on + strand, within Psyr_0511 at 567.411 kb on + strand, within Psyr_0511 at 567.411 kb on + strand, within Psyr_0511 at 567.412 kb on - strand, within Psyr_0511 at 567.420 kb on + strand, within Psyr_0511 at 567.421 kb on - strand, within Psyr_0511 at 567.503 kb on + strand, within Psyr_0511 at 567.504 kb on - strand, within Psyr_0511 at 567.658 kb on - strand at 567.758 kb on + strand, within Psyr_0512 at 567.761 kb on + strand, within Psyr_0512 at 567.761 kb on + strand, within Psyr_0512 at 567.762 kb on - strand, within Psyr_0512 at 567.787 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.791 kb on + strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 567.792 kb on - strand, within Psyr_0512 at 568.022 kb on + strand, within Psyr_0512 at 568.023 kb on - strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.166 kb on + strand at 568.166 kb on + strand at 568.167 kb on - strand at 568.172 kb on + strand at 568.252 kb on - strand at 568.252 kb on - strand at 568.252 kb on - strand at 568.275 kb on + strand at 568.275 kb on + strand at 568.276 kb on - strand at 568.276 kb on - strand at 568.276 kb on - strand at 568.322 kb on + strand at 568.322 kb on + strand at 568.322 kb on + strand at 568.323 kb on - strand at 568.323 kb on - strand at 568.323 kb on - strand at 568.544 kb on + strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.634 kb on - strand, within Psyr_0513 at 568.634 kb on - strand, within Psyr_0513 at 568.661 kb on + strand, within Psyr_0513 at 568.662 kb on - strand, within Psyr_0513 at 568.662 kb on - strand, within Psyr_0513
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 566,545 + Psyr_0509 0.89 +0.8 566,584 + +0.9 566,585 - -0.9 566,783 + +0.1 566,783 + -0.9 566,783 + -1.4 566,784 - +0.6 566,784 - +1.5 566,784 - -0.7 566,784 - -0.8 566,849 - +0.5 566,921 - Psyr_0510 0.20 +1.0 566,974 + Psyr_0510 0.30 -1.1 567,239 - Psyr_0510 0.80 +0.0 567,239 - Psyr_0510 0.80 -0.6 567,239 - Psyr_0510 0.80 -1.2 567,239 - Psyr_0510 0.80 -0.3 567,299 - -0.1 567,308 - +0.2 567,399 + Psyr_0511 0.10 -1.3 567,411 + Psyr_0511 0.14 -1.1 567,411 + Psyr_0511 0.14 +0.2 567,411 + Psyr_0511 0.14 -1.3 567,412 - Psyr_0511 0.14 -0.7 567,420 + Psyr_0511 0.17 -0.1 567,421 - Psyr_0511 0.18 -0.6 567,503 + Psyr_0511 0.45 +0.9 567,504 - Psyr_0511 0.45 +0.9 567,658 - -0.0 567,758 + Psyr_0512 0.19 -0.7 567,761 + Psyr_0512 0.19 +0.9 567,761 + Psyr_0512 0.19 -0.2 567,762 - Psyr_0512 0.19 +0.7 567,787 + Psyr_0512 0.25 -1.7 567,791 + Psyr_0512 0.26 +0.9 567,791 + Psyr_0512 0.26 +1.3 567,791 + Psyr_0512 0.26 +1.4 567,791 + Psyr_0512 0.26 +0.6 567,791 + Psyr_0512 0.26 +2.1 567,791 + Psyr_0512 0.26 +0.2 567,791 + Psyr_0512 0.26 -1.5 567,791 + Psyr_0512 0.26 -1.9 567,791 + Psyr_0512 0.26 -1.0 567,791 + Psyr_0512 0.26 -1.0 567,791 + Psyr_0512 0.26 -0.3 567,792 - Psyr_0512 0.26 -1.2 567,792 - Psyr_0512 0.26 -0.7 567,792 - Psyr_0512 0.26 +0.5 567,792 - Psyr_0512 0.26 -1.2 567,792 - Psyr_0512 0.26 -0.3 567,792 - Psyr_0512 0.26 +0.4 567,792 - Psyr_0512 0.26 +0.7 567,792 - Psyr_0512 0.26 +0.5 568,022 + Psyr_0512 0.76 -0.1 568,023 - Psyr_0512 0.76 +0.4 568,076 + Psyr_0512 0.88 -0.2 568,076 + Psyr_0512 0.88 -0.5 568,076 + Psyr_0512 0.88 -0.3 568,076 + Psyr_0512 0.88 -0.3 568,076 + Psyr_0512 0.88 -2.1 568,077 - Psyr_0512 0.88 +0.5 568,077 - Psyr_0512 0.88 +0.2 568,077 - Psyr_0512 0.88 +0.2 568,166 + -1.6 568,166 + -1.0 568,167 - -1.9 568,172 + -2.1 568,252 - -2.1 568,252 - -0.2 568,252 - -0.5 568,275 + +1.2 568,275 + +0.3 568,276 - -0.8 568,276 - +0.3 568,276 - +0.3 568,322 + +1.4 568,322 + -0.6 568,322 + +0.6 568,323 - -0.4 568,323 - -1.0 568,323 - +1.0 568,544 + Psyr_0513 0.25 -0.7 568,545 - Psyr_0513 0.25 -0.8 568,545 - Psyr_0513 0.25 -0.2 568,545 - Psyr_0513 0.25 +0.5 568,634 - Psyr_0513 0.37 +0.1 568,634 - Psyr_0513 0.37 -1.1 568,661 + Psyr_0513 0.40 +1.0 568,662 - Psyr_0513 0.40 +1.2 568,662 - Psyr_0513 0.40 -0.5
Or see this region's nucleotide sequence