Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0419 and Psyr_0420 are separated by 77 nucleotides Psyr_0420 and Psyr_0421 are separated by 115 nucleotides
Psyr_0419: Psyr_0419 - conserved hypothetical protein, at 457,693 to 458,958
_0419
Psyr_0420: Psyr_0420 - General substrate transporter:Major facilitator superfamily, at 459,036 to 460,304
_0420
Psyr_0421: Psyr_0421 - transcriptional regulator, LysR family, at 460,420 to 461,310
_0421
Position (kb) 459
460
461 Strain fitness (log2 ratio) -2
-1
0
1
2 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.123 kb on - strand, within Psyr_0419 at 458.123 kb on - strand, within Psyr_0419 at 458.123 kb on - strand, within Psyr_0419 at 458.150 kb on - strand, within Psyr_0419 at 458.161 kb on - strand, within Psyr_0419 at 458.276 kb on + strand, within Psyr_0419 at 458.276 kb on + strand, within Psyr_0419 at 458.277 kb on - strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.457 kb on - strand, within Psyr_0419 at 458.457 kb on - strand, within Psyr_0419 at 458.503 kb on + strand, within Psyr_0419 at 458.538 kb on - strand, within Psyr_0419 at 458.538 kb on - strand, within Psyr_0419 at 458.549 kb on - strand, within Psyr_0419 at 458.549 kb on - strand, within Psyr_0419 at 458.602 kb on + strand, within Psyr_0419 at 458.602 kb on + strand, within Psyr_0419 at 458.656 kb on + strand, within Psyr_0419 at 458.656 kb on + strand, within Psyr_0419 at 458.656 kb on + strand, within Psyr_0419 at 458.657 kb on - strand, within Psyr_0419 at 458.711 kb on + strand, within Psyr_0419 at 458.747 kb on - strand, within Psyr_0419 at 458.809 kb on + strand, within Psyr_0419 at 458.809 kb on + strand, within Psyr_0419 at 458.810 kb on - strand, within Psyr_0419 at 458.810 kb on - strand, within Psyr_0419 at 458.919 kb on - strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.998 kb on - strand at 458.998 kb on - strand at 458.998 kb on - strand at 458.998 kb on - strand at 459.027 kb on + strand at 459.027 kb on + strand at 459.027 kb on + strand at 459.028 kb on - strand at 459.028 kb on - strand at 459.028 kb on - strand at 459.028 kb on - strand at 459.111 kb on + strand at 459.111 kb on + strand at 459.111 kb on + strand at 459.112 kb on - strand at 459.112 kb on - strand at 459.143 kb on - strand at 459.191 kb on - strand, within Psyr_0420 at 459.193 kb on + strand, within Psyr_0420 at 459.194 kb on - strand, within Psyr_0420 at 459.194 kb on - strand, within Psyr_0420 at 459.205 kb on + strand, within Psyr_0420 at 459.205 kb on + strand, within Psyr_0420 at 459.205 kb on + strand, within Psyr_0420 at 459.206 kb on - strand, within Psyr_0420 at 459.206 kb on - strand, within Psyr_0420 at 459.399 kb on + strand, within Psyr_0420 at 459.400 kb on - strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.451 kb on + strand, within Psyr_0420 at 459.451 kb on + strand, within Psyr_0420 at 459.451 kb on + strand, within Psyr_0420 at 459.452 kb on - strand, within Psyr_0420 at 459.452 kb on - strand, within Psyr_0420 at 459.493 kb on + strand, within Psyr_0420 at 459.494 kb on - strand, within Psyr_0420 at 459.523 kb on + strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.745 kb on + strand, within Psyr_0420 at 459.745 kb on + strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.859 kb on + strand, within Psyr_0420 at 459.859 kb on + strand, within Psyr_0420 at 459.860 kb on - strand, within Psyr_0420 at 459.860 kb on - strand, within Psyr_0420 at 459.900 kb on + strand, within Psyr_0420 at 459.900 kb on + strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.145 kb on - strand, within Psyr_0420 at 460.145 kb on - strand, within Psyr_0420 at 460.145 kb on - strand, within Psyr_0420 at 460.154 kb on + strand, within Psyr_0420 at 460.154 kb on + strand, within Psyr_0420 at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.255 kb on - strand at 460.346 kb on + strand at 460.346 kb on + strand at 460.346 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.372 kb on + strand at 460.374 kb on + strand at 460.397 kb on + strand at 460.398 kb on - strand at 460.441 kb on + strand at 460.442 kb on - strand at 460.728 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 461.020 kb on + strand, within Psyr_0421 at 461.020 kb on + strand, within Psyr_0421 at 461.021 kb on - strand, within Psyr_0421 at 461.021 kb on - strand, within Psyr_0421 at 461.031 kb on + strand, within Psyr_0421 at 461.032 kb on - strand, within Psyr_0421 at 461.035 kb on + strand, within Psyr_0421 at 461.035 kb on + strand, within Psyr_0421 at 461.035 kb on + strand, within Psyr_0421 at 461.036 kb on - strand, within Psyr_0421 at 461.303 kb on + strand at 461.303 kb on + strand at 461.303 kb on + strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 458,122 + Psyr_0419 0.34 -0.7 458,122 + Psyr_0419 0.34 -1.7 458,122 + Psyr_0419 0.34 -0.1 458,122 + Psyr_0419 0.34 -0.1 458,122 + Psyr_0419 0.34 +0.2 458,123 - Psyr_0419 0.34 +0.6 458,123 - Psyr_0419 0.34 +0.1 458,123 - Psyr_0419 0.34 +0.9 458,150 - Psyr_0419 0.36 +0.2 458,161 - Psyr_0419 0.37 -1.0 458,276 + Psyr_0419 0.46 +0.2 458,276 + Psyr_0419 0.46 -0.8 458,277 - Psyr_0419 0.46 -1.0 458,281 + Psyr_0419 0.46 -0.3 458,281 + Psyr_0419 0.46 -0.6 458,281 + Psyr_0419 0.46 +0.5 458,281 + Psyr_0419 0.46 -1.2 458,282 - Psyr_0419 0.47 +0.3 458,282 - Psyr_0419 0.47 -0.5 458,282 - Psyr_0419 0.47 +0.6 458,282 - Psyr_0419 0.47 -0.6 458,282 - Psyr_0419 0.47 +0.6 458,282 - Psyr_0419 0.47 -0.7 458,282 - Psyr_0419 0.47 +0.1 458,456 + Psyr_0419 0.60 -0.5 458,456 + Psyr_0419 0.60 -0.5 458,456 + Psyr_0419 0.60 +0.2 458,456 + Psyr_0419 0.60 -1.3 458,456 + Psyr_0419 0.60 -0.0 458,456 + Psyr_0419 0.60 +0.6 458,456 + Psyr_0419 0.60 -0.4 458,456 + Psyr_0419 0.60 -0.7 458,456 + Psyr_0419 0.60 -1.3 458,457 - Psyr_0419 0.60 -0.4 458,457 - Psyr_0419 0.60 +1.5 458,503 + Psyr_0419 0.64 -0.3 458,538 - Psyr_0419 0.67 +0.1 458,538 - Psyr_0419 0.67 +1.9 458,549 - Psyr_0419 0.68 -0.4 458,549 - Psyr_0419 0.68 -0.1 458,602 + Psyr_0419 0.72 -0.9 458,602 + Psyr_0419 0.72 +0.1 458,656 + Psyr_0419 0.76 -1.0 458,656 + Psyr_0419 0.76 +0.2 458,656 + Psyr_0419 0.76 +0.2 458,657 - Psyr_0419 0.76 +0.5 458,711 + Psyr_0419 0.80 -0.5 458,747 - Psyr_0419 0.83 +2.2 458,809 + Psyr_0419 0.88 +0.4 458,809 + Psyr_0419 0.88 +0.5 458,810 - Psyr_0419 0.88 +0.6 458,810 - Psyr_0419 0.88 -0.1 458,919 - -0.6 458,997 + +1.2 458,997 + +0.5 458,997 + +2.0 458,997 + +0.7 458,997 + -0.4 458,998 - +0.5 458,998 - +0.6 458,998 - +0.2 458,998 - +1.5 459,027 + -2.2 459,027 + -2.1 459,027 + +0.2 459,028 - -0.3 459,028 - -0.4 459,028 - -2.0 459,028 - -1.7 459,111 + -0.4 459,111 + +0.6 459,111 + -0.7 459,112 - -1.7 459,112 - -0.5 459,143 - +0.2 459,191 - Psyr_0420 0.12 +0.7 459,193 + Psyr_0420 0.12 +1.1 459,194 - Psyr_0420 0.12 +0.9 459,194 - Psyr_0420 0.12 +0.2 459,205 + Psyr_0420 0.13 -1.0 459,205 + Psyr_0420 0.13 +0.3 459,205 + Psyr_0420 0.13 +0.2 459,206 - Psyr_0420 0.13 -0.3 459,206 - Psyr_0420 0.13 -0.6 459,399 + Psyr_0420 0.29 +1.3 459,400 - Psyr_0420 0.29 -0.1 459,436 + Psyr_0420 0.32 -0.3 459,436 + Psyr_0420 0.32 +0.5 459,436 + Psyr_0420 0.32 +0.5 459,436 + Psyr_0420 0.32 -1.1 459,436 + Psyr_0420 0.32 -1.7 459,436 + Psyr_0420 0.32 -2.1 459,436 + Psyr_0420 0.32 -0.3 459,436 + Psyr_0420 0.32 -0.2 459,437 - Psyr_0420 0.32 +0.0 459,437 - Psyr_0420 0.32 +0.9 459,437 - Psyr_0420 0.32 -0.0 459,437 - Psyr_0420 0.32 +0.2 459,451 + Psyr_0420 0.33 -1.6 459,451 + Psyr_0420 0.33 +0.3 459,451 + Psyr_0420 0.33 +0.9 459,452 - Psyr_0420 0.33 -0.5 459,452 - Psyr_0420 0.33 -1.2 459,493 + Psyr_0420 0.36 +0.9 459,494 - Psyr_0420 0.36 -2.4 459,523 + Psyr_0420 0.38 +1.5 459,524 - Psyr_0420 0.38 +0.3 459,524 - Psyr_0420 0.38 -0.9 459,524 - Psyr_0420 0.38 +1.4 459,524 - Psyr_0420 0.38 -1.3 459,524 - Psyr_0420 0.38 -0.4 459,524 - Psyr_0420 0.38 +0.9 459,524 - Psyr_0420 0.38 +1.9 459,745 + Psyr_0420 0.56 +1.3 459,745 + Psyr_0420 0.56 +1.0 459,746 - Psyr_0420 0.56 -0.2 459,746 - Psyr_0420 0.56 +0.8 459,746 - Psyr_0420 0.56 +1.3 459,746 - Psyr_0420 0.56 +1.4 459,746 - Psyr_0420 0.56 +0.7 459,746 - Psyr_0420 0.56 -0.9 459,746 - Psyr_0420 0.56 +0.3 459,808 + Psyr_0420 0.61 -0.1 459,808 + Psyr_0420 0.61 -0.2 459,808 + Psyr_0420 0.61 -0.2 459,808 + Psyr_0420 0.61 +0.1 459,808 + Psyr_0420 0.61 +1.1 459,808 + Psyr_0420 0.61 -0.4 459,808 + Psyr_0420 0.61 -0.4 459,808 + Psyr_0420 0.61 +0.4 459,808 + Psyr_0420 0.61 -1.4 459,809 - Psyr_0420 0.61 +1.9 459,809 - Psyr_0420 0.61 -0.8 459,809 - Psyr_0420 0.61 +0.2 459,809 - Psyr_0420 0.61 -0.4 459,809 - Psyr_0420 0.61 +0.3 459,809 - Psyr_0420 0.61 +1.5 459,809 - Psyr_0420 0.61 +1.9 459,809 - Psyr_0420 0.61 -0.9 459,809 - Psyr_0420 0.61 -0.1 459,809 - Psyr_0420 0.61 -0.1 459,809 - Psyr_0420 0.61 +0.1 459,859 + Psyr_0420 0.65 +0.0 459,859 + Psyr_0420 0.65 -0.5 459,860 - Psyr_0420 0.65 +1.9 459,860 - Psyr_0420 0.65 +0.5 459,900 + Psyr_0420 0.68 -1.7 459,900 + Psyr_0420 0.68 +1.5 459,901 - Psyr_0420 0.68 -1.3 459,901 - Psyr_0420 0.68 -0.2 459,901 - Psyr_0420 0.68 +0.8 459,901 - Psyr_0420 0.68 +0.4 459,901 - Psyr_0420 0.68 +0.1 459,901 - Psyr_0420 0.68 -0.8 459,901 - Psyr_0420 0.68 +0.3 460,144 + Psyr_0420 0.87 -0.6 460,144 + Psyr_0420 0.87 +0.1 460,144 + Psyr_0420 0.87 +1.2 460,144 + Psyr_0420 0.87 +0.4 460,145 - Psyr_0420 0.87 +1.9 460,145 - Psyr_0420 0.87 -0.5 460,145 - Psyr_0420 0.87 +1.9 460,154 + Psyr_0420 0.88 +0.7 460,154 + Psyr_0420 0.88 +0.1 460,225 + +0.9 460,225 + -0.8 460,225 + -0.9 460,225 + -0.7 460,225 + +0.3 460,225 + -0.0 460,225 + +0.9 460,225 + +0.8 460,225 + +0.5 460,225 + +1.1 460,225 + -1.0 460,225 + +1.5 460,225 + -0.1 460,225 + -0.7 460,226 - -0.2 460,226 - -1.0 460,226 - +0.1 460,226 - +0.7 460,226 - +0.7 460,226 - -1.0 460,226 - +0.7 460,226 - -0.1 460,226 - +0.0 460,226 - -1.1 460,255 - -1.2 460,346 + -0.5 460,346 + +0.6 460,346 + -2.6 460,348 + +0.5 460,348 + -0.5 460,348 + +0.9 460,348 + +0.8 460,348 + -0.1 460,348 + -0.4 460,348 + +0.7 460,348 + -0.7 460,348 + -1.2 460,348 + +0.2 460,348 + -0.1 460,348 + +0.9 460,348 + -1.7 460,348 + -0.6 460,348 + -0.1 460,348 + +0.4 460,349 - -0.2 460,349 - -1.3 460,349 - +0.6 460,349 - +0.2 460,349 - -0.7 460,349 - -1.7 460,349 - +0.6 460,349 - +0.3 460,349 - +0.8 460,349 - -0.2 460,349 - +1.0 460,349 - +0.0 460,349 - -0.2 460,349 - -0.3 460,372 + -0.3 460,374 + +0.1 460,397 + -0.3 460,398 - +0.0 460,441 + -0.7 460,442 - -0.9 460,728 + Psyr_0421 0.35 +0.2 460,972 + Psyr_0421 0.62 -1.9 460,972 + Psyr_0421 0.62 -0.2 460,972 + Psyr_0421 0.62 -1.7 460,972 + Psyr_0421 0.62 +0.1 460,972 + Psyr_0421 0.62 +0.7 460,972 + Psyr_0421 0.62 +0.2 460,972 + Psyr_0421 0.62 +0.8 460,972 + Psyr_0421 0.62 +0.0 460,973 - Psyr_0421 0.62 -0.7 460,973 - Psyr_0421 0.62 -0.1 460,973 - Psyr_0421 0.62 -0.3 460,973 - Psyr_0421 0.62 -0.1 460,973 - Psyr_0421 0.62 +0.0 460,973 - Psyr_0421 0.62 -0.1 460,973 - Psyr_0421 0.62 -0.5 460,973 - Psyr_0421 0.62 +1.1 460,973 - Psyr_0421 0.62 +0.8 460,973 - Psyr_0421 0.62 -1.1 460,973 - Psyr_0421 0.62 +0.5 460,973 - Psyr_0421 0.62 +0.9 460,973 - Psyr_0421 0.62 +0.7 461,020 + Psyr_0421 0.67 +0.0 461,020 + Psyr_0421 0.67 +0.0 461,021 - Psyr_0421 0.67 -0.5 461,021 - Psyr_0421 0.67 -0.3 461,031 + Psyr_0421 0.69 +2.7 461,032 - Psyr_0421 0.69 +0.5 461,035 + Psyr_0421 0.69 +0.9 461,035 + Psyr_0421 0.69 -1.1 461,035 + Psyr_0421 0.69 +1.1 461,036 - Psyr_0421 0.69 -0.9 461,303 + -1.7 461,303 + +1.8 461,303 + +0.5 461,304 - -0.0 461,304 - +0.4 461,304 - +0.3 461,304 - -1.1 461,304 - +0.7 461,304 - +0.3 461,304 - +0.4 461,304 - -0.1
Or see this region's nucleotide sequence