Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0230 and Psyr_0231 are separated by 175 nucleotides Psyr_0231 and Psyr_0232 are separated by 2 nucleotides Psyr_0232 and Psyr_0233 are separated by 105 nucleotides Psyr_0233 and Psyr_0234 are separated by 7 nucleotides
Psyr_0230: Psyr_0230 - transcriptional regulator, GntR family, at 244,835 to 246,256
_0230
Psyr_0231: Psyr_0231 - conserved hypothetical protein, at 246,432 to 246,683
_0231
Psyr_0232: Psyr_0232 - gamma-glutamylputrescine oxidase, at 246,686 to 247,999
_0232
Psyr_0233: Psyr_0233 - Protein of unknown function UPF0153, at 248,105 to 248,458
_0233
Psyr_0234: Psyr_0234 - transcriptional regulator, AsnC family, at 248,466 to 248,954
_0234
Position (kb) 246
247
248 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 245.820 kb on + strand, within Psyr_0230 at 245.820 kb on + strand, within Psyr_0230 at 245.821 kb on - strand, within Psyr_0230 at 245.835 kb on + strand, within Psyr_0230 at 245.995 kb on + strand, within Psyr_0230 at 246.067 kb on - strand, within Psyr_0230 at 246.137 kb on - strand at 246.137 kb on - strand at 246.199 kb on - strand at 246.199 kb on - strand at 246.199 kb on - strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.247 kb on - strand at 246.261 kb on + strand at 246.310 kb on - strand at 246.311 kb on + strand at 246.312 kb on - strand at 246.312 kb on - strand at 246.312 kb on - strand at 246.350 kb on + strand at 246.351 kb on - strand at 246.562 kb on - strand, within Psyr_0231 at 246.562 kb on - strand, within Psyr_0231 at 246.562 kb on - strand, within Psyr_0231 at 246.876 kb on + strand, within Psyr_0232 at 246.876 kb on + strand, within Psyr_0232 at 246.877 kb on - strand, within Psyr_0232 at 246.877 kb on - strand, within Psyr_0232 at 246.888 kb on + strand, within Psyr_0232 at 246.888 kb on + strand, within Psyr_0232 at 246.888 kb on + strand, within Psyr_0232 at 246.889 kb on - strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.920 kb on + strand, within Psyr_0232 at 246.921 kb on - strand, within Psyr_0232 at 247.026 kb on + strand, within Psyr_0232 at 247.026 kb on + strand, within Psyr_0232 at 247.026 kb on + strand, within Psyr_0232 at 247.027 kb on - strand, within Psyr_0232 at 247.027 kb on - strand, within Psyr_0232 at 247.027 kb on - strand, within Psyr_0232 at 247.107 kb on + strand, within Psyr_0232 at 247.107 kb on + strand, within Psyr_0232 at 247.108 kb on - strand, within Psyr_0232 at 247.108 kb on - strand, within Psyr_0232 at 247.110 kb on + strand, within Psyr_0232 at 247.110 kb on + strand, within Psyr_0232 at 247.111 kb on - strand, within Psyr_0232 at 247.137 kb on - strand, within Psyr_0232 at 247.137 kb on - strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.257 kb on + strand, within Psyr_0232 at 247.257 kb on + strand, within Psyr_0232 at 247.310 kb on - strand, within Psyr_0232 at 247.461 kb on + strand, within Psyr_0232 at 247.944 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.948 kb on - strand at 247.948 kb on - strand at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.419 kb on - strand, within Psyr_0233 at 248.419 kb on - strand, within Psyr_0233 at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.448 kb on + strand at 248.448 kb on + strand at 248.449 kb on - strand at 248.449 kb on - strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.457 kb on - strand at 248.457 kb on - strand at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.614 kb on + strand, within Psyr_0234 at 248.615 kb on - strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.835 kb on - strand, within Psyr_0234 at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 245,820 + Psyr_0230 0.69 -0.7 245,820 + Psyr_0230 0.69 -1.0 245,821 - Psyr_0230 0.69 -1.5 245,835 + Psyr_0230 0.70 +0.5 245,995 + Psyr_0230 0.82 +0.8 246,067 - Psyr_0230 0.87 -0.2 246,137 - -0.2 246,137 - -0.2 246,199 - -0.5 246,199 - +0.1 246,199 - +0.1 246,246 + -0.3 246,246 + +2.8 246,246 + +0.6 246,246 + -1.4 246,246 + +0.5 246,247 - +0.8 246,261 + -0.5 246,310 - -1.4 246,311 + -0.0 246,312 - -1.0 246,312 - -0.6 246,312 - -0.2 246,350 + +0.2 246,351 - +0.8 246,562 - Psyr_0231 0.52 -1.0 246,562 - Psyr_0231 0.52 -0.5 246,562 - Psyr_0231 0.52 -1.1 246,876 + Psyr_0232 0.14 +0.4 246,876 + Psyr_0232 0.14 -0.8 246,877 - Psyr_0232 0.15 -0.4 246,877 - Psyr_0232 0.15 -0.2 246,888 + Psyr_0232 0.15 -1.4 246,888 + Psyr_0232 0.15 -0.9 246,888 + Psyr_0232 0.15 +0.2 246,889 - Psyr_0232 0.15 +0.3 246,893 + Psyr_0232 0.16 +0.3 246,893 + Psyr_0232 0.16 -0.5 246,893 + Psyr_0232 0.16 -1.5 246,893 + Psyr_0232 0.16 -0.3 246,893 + Psyr_0232 0.16 -1.0 246,893 + Psyr_0232 0.16 -1.3 246,893 + Psyr_0232 0.16 -2.2 246,894 - Psyr_0232 0.16 +0.8 246,894 - Psyr_0232 0.16 -1.5 246,894 - Psyr_0232 0.16 -0.7 246,894 - Psyr_0232 0.16 -1.1 246,894 - Psyr_0232 0.16 -2.2 246,894 - Psyr_0232 0.16 -1.3 246,894 - Psyr_0232 0.16 -1.7 246,920 + Psyr_0232 0.18 -1.0 246,921 - Psyr_0232 0.18 -2.0 247,026 + Psyr_0232 0.26 -1.7 247,026 + Psyr_0232 0.26 -0.6 247,026 + Psyr_0232 0.26 +0.1 247,027 - Psyr_0232 0.26 +0.2 247,027 - Psyr_0232 0.26 -0.8 247,027 - Psyr_0232 0.26 +0.4 247,107 + Psyr_0232 0.32 +0.3 247,107 + Psyr_0232 0.32 -2.6 247,108 - Psyr_0232 0.32 -0.2 247,108 - Psyr_0232 0.32 -0.3 247,110 + Psyr_0232 0.32 -0.1 247,110 + Psyr_0232 0.32 +0.0 247,111 - Psyr_0232 0.32 +0.1 247,137 - Psyr_0232 0.34 +0.1 247,137 - Psyr_0232 0.34 +0.8 247,203 + Psyr_0232 0.39 +0.6 247,203 + Psyr_0232 0.39 +0.2 247,203 + Psyr_0232 0.39 -1.7 247,203 + Psyr_0232 0.39 +0.5 247,203 + Psyr_0232 0.39 +0.6 247,204 - Psyr_0232 0.39 -2.3 247,204 - Psyr_0232 0.39 +0.4 247,204 - Psyr_0232 0.39 -0.7 247,204 - Psyr_0232 0.39 -0.3 247,204 - Psyr_0232 0.39 -0.2 247,204 - Psyr_0232 0.39 +0.3 247,204 - Psyr_0232 0.39 +0.2 247,204 - Psyr_0232 0.39 +0.6 247,204 - Psyr_0232 0.39 -0.8 247,257 + Psyr_0232 0.43 -0.1 247,257 + Psyr_0232 0.43 -0.2 247,310 - Psyr_0232 0.47 -0.3 247,461 + Psyr_0232 0.59 +0.0 247,944 + -0.2 247,947 + +0.5 247,947 + +0.4 247,947 + +0.1 247,947 + +0.2 247,947 + +0.5 247,948 - -0.1 247,948 - +0.1 248,280 + Psyr_0233 0.49 -0.3 248,280 + Psyr_0233 0.49 +3.8 248,280 + Psyr_0233 0.49 +0.5 248,280 + Psyr_0233 0.49 +0.3 248,281 - Psyr_0233 0.50 -0.3 248,281 - Psyr_0233 0.50 +0.3 248,281 - Psyr_0233 0.50 +2.4 248,309 + Psyr_0233 0.58 +0.3 248,309 + Psyr_0233 0.58 +0.1 248,309 + Psyr_0233 0.58 +1.0 248,310 - Psyr_0233 0.58 +0.2 248,310 - Psyr_0233 0.58 -0.5 248,310 - Psyr_0233 0.58 -1.7 248,418 + Psyr_0233 0.88 -0.3 248,418 + Psyr_0233 0.88 +0.1 248,418 + Psyr_0233 0.88 +0.3 248,419 - Psyr_0233 0.89 +0.3 248,419 - Psyr_0233 0.89 -1.1 248,426 + +0.3 248,426 + +0.8 248,426 + -0.7 248,426 + +1.6 248,426 + +0.5 248,426 + -0.6 248,426 + -1.5 248,426 + -2.5 248,426 + +0.2 248,426 + -1.3 248,426 + +0.8 248,426 + -2.2 248,426 + +0.3 248,426 + -0.7 248,427 - -0.4 248,427 - +0.0 248,427 - -0.1 248,427 - -1.6 248,427 - -0.6 248,427 - +0.4 248,427 - -0.5 248,427 - -1.7 248,427 - +0.4 248,427 - +0.8 248,427 - -1.3 248,427 - -0.6 248,427 - -1.2 248,427 - +1.0 248,428 + +0.8 248,428 + -0.5 248,428 + +0.1 248,428 + -0.9 248,428 + +0.3 248,428 + -0.2 248,428 + -0.2 248,428 + -0.1 248,429 - -0.5 248,429 - -0.2 248,429 - -0.9 248,429 - -0.3 248,429 - -0.2 248,429 - +0.8 248,429 - -0.9 248,429 - -0.4 248,429 - -0.8 248,448 + -0.6 248,448 + -0.7 248,449 - +0.1 248,449 - +0.1 248,454 + +1.4 248,454 + +0.1 248,454 + -0.6 248,454 + +1.0 248,454 + -0.3 248,454 + -1.2 248,454 + +0.0 248,455 - -1.0 248,455 - +0.1 248,455 - +0.4 248,455 - +2.8 248,455 - -0.7 248,455 - +1.0 248,456 + -0.2 248,456 + +0.8 248,456 + +0.3 248,456 + +0.0 248,456 + -1.5 248,457 - +0.6 248,457 - -0.1 248,518 + Psyr_0234 0.11 +0.5 248,518 + Psyr_0234 0.11 -0.2 248,518 + Psyr_0234 0.11 -0.8 248,518 + Psyr_0234 0.11 +0.6 248,518 + Psyr_0234 0.11 -2.6 248,518 + Psyr_0234 0.11 -0.0 248,518 + Psyr_0234 0.11 -0.1 248,518 + Psyr_0234 0.11 -0.5 248,518 + Psyr_0234 0.11 -0.6 248,518 + Psyr_0234 0.11 -0.7 248,518 + Psyr_0234 0.11 -1.6 248,518 + Psyr_0234 0.11 -0.6 248,519 - Psyr_0234 0.11 -1.7 248,519 - Psyr_0234 0.11 -1.0 248,519 - Psyr_0234 0.11 +0.2 248,519 - Psyr_0234 0.11 -0.0 248,519 - Psyr_0234 0.11 -0.5 248,519 - Psyr_0234 0.11 -2.4 248,519 - Psyr_0234 0.11 +0.8 248,519 - Psyr_0234 0.11 -1.1 248,519 - Psyr_0234 0.11 +0.4 248,519 - Psyr_0234 0.11 -0.7 248,519 - Psyr_0234 0.11 -1.6 248,519 - Psyr_0234 0.11 -0.6 248,614 + Psyr_0234 0.30 -0.0 248,615 - Psyr_0234 0.30 -2.4 248,707 + Psyr_0234 0.49 -0.7 248,707 + Psyr_0234 0.49 -0.9 248,707 + Psyr_0234 0.49 -0.4 248,707 + Psyr_0234 0.49 +0.8 248,707 + Psyr_0234 0.49 -0.5 248,708 - Psyr_0234 0.49 -0.2 248,708 - Psyr_0234 0.49 -0.2 248,708 - Psyr_0234 0.49 -0.2 248,708 - Psyr_0234 0.49 -0.3 248,708 - Psyr_0234 0.49 -1.9 248,835 - Psyr_0234 0.75 +1.4 248,913 + +0.3 248,913 + -0.8 248,913 + -0.2 248,913 + -1.3 248,913 + -1.0 248,913 + -0.0 248,913 + -0.7 248,913 + -1.6 248,913 + -0.3 248,913 + +0.6 248,913 + +0.7 248,914 - -0.5 248,914 - -0.4 248,914 - -1.2 248,914 - -0.6 248,914 - +1.4 248,914 - -0.6 248,914 - -0.2 248,914 - -0.5 248,914 - +0.8 248,914 - +1.4
Or see this region's nucleotide sequence